Literature DB >> 35412252

Visualizing Nuclear Pore Complexes in Xenopus Egg Extracts.

Sampada Mishra1, Daniel L Levy2.   

Abstract

The nuclear pore complex (NPC) is the conduit in the nuclear envelope through which proteins and RNA are transported between the cytoplasm and nucleus. Xenopus egg extracts that support de novo assembly of nuclei have provided a robust system to study NPC structure and function because the biochemical composition of the extract can be easily manipulated. Here we describe how to assemble nuclei in Xenopus egg extract, how to visualize and analyze NPCs in both live and fixed samples, and different approaches to altering nucleocytoplasmic transport in extract.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Immunofluorescence; NPC; Nuclear import; Nuclear pore complexes; Spindown; Xenopus egg extracts; mAb414

Mesh：

Substances：

Year:  2022        PMID： 35412252      PMCID： PMC9219414          DOI： 10.1007/978-1-0716-2337-4_25

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  33 in total

1.  ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division.

Authors:  Beth A Rasala; Arturo V Orjalo; Zhouxin Shen; Steven Briggs; Douglass J Forbes
Journal:  Proc Natl Acad Sci U S A       Date:  2006-11-10       Impact factor: 11.205

2.  Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts.

Authors:  Eva Hannak; Rebecca Heald
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

3.  Importazole, a small molecule inhibitor of the transport receptor importin-β.

Authors:  Jonathan F Soderholm; Stephen L Bird; Petr Kalab; Yasaswini Sampathkumar; Keisuke Hasegawa; Michael Uehara-Bingen; Karsten Weis; Rebecca Heald
Journal:  ACS Chem Biol       Date:  2011-04-21       Impact factor: 5.100

4.  The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.

Authors:  A W Murray; M J Solomon; M W Kirschner
Journal:  Nature       Date:  1989-05-25       Impact factor: 49.962

5.  Isolation and Demembranation of Xenopus Sperm Nuclei.

Authors:  James W Hazel; Jesse C Gatlin
Journal:  Cold Spring Harb Protoc       Date:  2018-06-01

6.  Nuclear size is regulated by importin α and Ntf2 in Xenopus.

Authors:  Daniel L Levy; Rebecca Heald
Journal:  Cell       Date:  2010-10-15       Impact factor: 41.582

7.  Transportin regulates major mitotic assembly events: from spindle to nuclear pore assembly.

Authors:  Corine K Lau; Valerie A Delmar; Rene C Chan; Quang Phung; Cyril Bernis; Boris Fichtman; Beth A Rasala; Douglass J Forbes
Journal:  Mol Biol Cell       Date:  2009-07-29       Impact factor: 4.138

8.  Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.

Authors:  J J Blow; R A Laskey
Journal:  Cell       Date:  1986-11-21       Impact factor: 41.582

9.  Developmentally regulated activity of CRM1/XPO1 during early Xenopus embryogenesis.

Authors:  M Callanan; N Kudo; S Gout; M Brocard; M Yoshida; S Dimitrov; S Khochbin
Journal:  J Cell Sci       Date:  2000-02       Impact factor: 5.285

10.  Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins.

Authors:  Peter J Gillespie; Agnieszka Gambus; J Julian Blow
Journal:  Methods       Date:  2012-04-19       Impact factor: 3.608
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  1 in total

1.  Nuclear F-actin and Lamin A antagonistically modulate nuclear shape.

Authors:  Sampada Mishra; Daniel L Levy
Journal:  J Cell Sci       Date:  2022-07-04       Impact factor: 5.235
  1 in total

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