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Literature DB >> 3537732

Anchoring a secreted plasmodium antigen on the surface of recombinant vaccinia virus-infected cells increases its immunogenicity.

C J Langford, S J Edwards, G L Smith, G F Mitchell, B Moss, D J Kemp, R F Anders.

Abstract

We show that the subcellular location of foreign antigens expressed in recombinant vaccinia viruses influences their effectiveness as immunogens. Live recombinant viruses induced very poor antibody responses to a secreted repetitive plasmodial antigen (the S-antigen) in rabbits and mice. The poor response accords with epidemiological data suggesting that S-antigens are poorly immunogenic. Appending the transmembrane domain of a membrane immunoglobulin (immunoglobulin G1) to its carboxy terminus produced a hybrid S-antigen that was no longer secreted but was located on the surface of virus-infected cells. This recombinant virus elicited high antibody titers to the S-antigen. This approach will facilitate the use of live virus delivery systems to immunize against a wide range of foreign nonsurface antigens.

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Year: 1986 PMID： 3537732 PMCID： PMC367055 DOI： 10.1128/mcb.6.9.3191-3199.1986

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

21 in total

Review 1. Antigenic repeat structures in proteins of Plasmodium falciparum.

Authors: R F Anders; P T Shi; D B Scanlon; S J Leach; R L Coppel; G V Brown; H D Stahl; D J Kemp
Journal: Ciba Found Symp Date: 1986

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Journal: Parasitology Date: 1975-10 Impact factor: 3.234

3. mRNA for surface immunoglobulin gamma chains encodes a highly conserved transmembrane sequence and a 28-residue intracellular domain.

Authors: B M Tyler; A F Cowman; S D Gerondakis; J M Adams; O Bernard
Journal: Proc Natl Acad Sci U S A Date: 1982-03 Impact factor: 11.205

4. Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen.

Authors: G L Smith; M Mackett; B Moss
Journal: Nature Date: 1983-04-07 Impact factor: 49.962

5. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.

Authors: M Kozak
Journal: Nucleic Acids Res Date: 1981-10-24 Impact factor: 16.971

6. Vaccinia virus: a selectable eukaryotic cloning and expression vector.

Authors: M Mackett; G L Smith; B Moss
Journal: Proc Natl Acad Sci U S A Date: 1982-12 Impact factor: 11.205

7. Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.

Authors: D Panicali; E Paoletti
Journal: Proc Natl Acad Sci U S A Date: 1982-08 Impact factor: 11.205

8. Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.

Authors: G L Smith; B R Murphy; B Moss
Journal: Proc Natl Acad Sci U S A Date: 1983-12 Impact factor: 11.205

9. Construction of live vaccines by using genetically engineered poxviruses: biological activity of recombinant vaccinia virus expressing influenza virus hemagglutinin.

Authors: D Panicali; S W Davis; R L Weinberg; E Paoletti
Journal: Proc Natl Acad Sci U S A Date: 1983-09 Impact factor: 11.205

10. Expression of Plasmodium falciparum blood-stage antigens in Escherichia coli: detection with antibodies from immune humans.

Authors: D J Kemp; R L Coppel; A F Cowman; R B Saint; G V Brown; R F Anders
Journal: Proc Natl Acad Sci U S A Date: 1983-06 Impact factor: 11.205

19 in total

1. Targeting vaccinia virus-expressed secretory beta subunit of human chorionic gonadotropin to the cell surface induces antibodies.

Authors: J Srinivasan; O Singh; S Chakrabarti; G P Talwar
Journal: Infect Immun Date: 1995-12 Impact factor: 3.441

2. The immunogenicity of VP7, a rotavirus antigen resident in the endoplasmic reticulum, is enhanced by cell surface expression.

Authors: M E Andrew; D B Boyle; P L Whitfeld; L J Lockett; I D Anthony; A R Bellamy; G W Both
Journal: J Virol Date: 1990-10 Impact factor: 5.103

Review 3. Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety.

Authors: B Moss
Journal: Proc Natl Acad Sci U S A Date: 1996-10-15 Impact factor: 11.205

4. Attenuated vaccinia virus-circumsporozoite protein recombinants confer protection against rodent malaria.

Authors: D E Lanar; J A Tine; C de Taisne; M C Seguin; W I Cox; J P Winslow; L A Ware; E B Kauffman; D Gordon; W R Ballou; E Paoletti; J C Sadoff
Journal: Infect Immun Date: 1996-05 Impact factor: 3.441

5. Membrane binding and endoplasmic reticulum retention sequences of rotavirus VP7 are distinct: role of carboxy-terminal and other residues in membrane binding.

Authors: M L Clarke; L J Lockett; G W Both
Journal: J Virol Date: 1995-10 Impact factor: 5.103

6. Assembly and analysis of a functional vaccinia virus "amplicon" containing the C-repeat region from the M protein of Streptococcus pyogenes.

Authors: D E Hruby; O Schneewind; E M Wilson; V A Fischetti
Journal: Proc Natl Acad Sci U S A Date: 1991-04-15 Impact factor: 11.205