| Literature DB >> 35361973 |
Le Gao1,2, Sang Liu1,2,3, Lingfeng Gou1, Yachuang Hu1,2, Yanhe Liu1,2, Li Deng1, Danyi Ma1,2, Haifang Wang1, Qiaoqiao Yang4, Zhaoqin Chen1, Dechen Liu1, Shou Qiu1,2, Xiaofei Wang1, Danying Wang1, Xinran Wang1, Biyu Ren1, Qingxu Liu1, Tianzhi Chen1, Xiaoxue Shi1, Haishan Yao1, Chun Xu1, Chengyu T Li1, Yangang Sun1, Anan Li5,6, Qingming Luo5,6,7, Hui Gong8,9, Ninglong Xu10,11,12, Jun Yan13,14,15.
Abstract
Prefrontal cortex (PFC) is the cognitive center that integrates and regulates global brain activity. However, the whole-brain organization of PFC axon projections remains poorly understood. Using single-neuron reconstruction of 6,357 mouse PFC projection neurons, we identified 64 projectome-defined subtypes. Each of four previously known major cortico-cortical subnetworks was targeted by a distinct group of PFC subtypes defined by their first-order axon collaterals. Further analysis unraveled topographic rules of soma distribution within PFC, first-order collateral branch point-dependent target selection and terminal arbor distribution-dependent target subdivision. Furthermore, we obtained a high-precision hierarchical map within PFC and three distinct functionally related PFC modules, each enriched with internal recurrent connectivity. Finally, we showed that each transcriptome subtype corresponds to multiple projectome subtypes found in different PFC subregions. Thus, whole-brain single-neuron projectome analysis reveals organization principles of axon projections within and outside PFC and provides the essential basis for elucidating neuronal connectivity underlying diverse PFC functions.Entities:
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Year: 2022 PMID： 35361973 DOI： 10.1038/s41593-022-01041-5
Source DB: PubMed Journal: Nat Neurosci ISSN： 1097-6256 Impact factor: 28.771