| Literature DB >> 35359706 |
Dalia K Hemdan1, Manar A Selim2, Rania A Galhom3, Mohamed H El Daharawy4, Hayam Y Hassan1.
Abstract
This study evaluated exposed dogs' dental pulp response after direct capping with autologous dental pulp stem cells (DPSCs) and compared the results with MTA (Angelus, Brazil). Sixty teeth from six mongrel dogs were included (n = 60), allocated into four groups; negative control (n = 6), positive control (n = 6), MTA (n = 24), and DPSCs (n = 24). DPSCs were isolated and cultured from extracted first premolars (1premolar/dog). The teeth were prepared for histological and immunohistochemical analyses. Statistical analysis was calculated using ANOVA tests, P ≤ 0.05. MTA induced the formation of an almost complete calcific bridge. DPSCs successfully regenerated the injured pulp and dentin. The comparison between the capping groups and time intervals had a statistically significant effect, as well as, the interaction between the two variables. DPSCs had greater ability in the reparative and regenerative process of dentin and odontoblastic differentiation by having a significantly stronger TGFβ1 expression than MTA.Entities:
Keywords: DPSCs; Direct pulp capping; Dogs' dental pulp; MTA; TGFβ1
Year: 2022 PMID： 35359706 PMCID： PMC8961227 DOI： 10.1016/j.jobcr.2022.03.009
Source DB: PubMed Journal: J Oral Biol Craniofac Res ISSN： 2212-4268