Literature DB >> 21198335

Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.

Pedro P Carvalho1, Xiying Wu, Gang Yu, Marilyn Dietrich, Isabel R Dias, Manuela E Gomes, Rui L Reis, Jeffrey M Gimble.   

Abstract

Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable.

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Year:  2011        PMID： 21198335     DOI： 10.3109/14653249.2010.544721

Source DB:  PubMed          Journal:  Cytotherapy        ISSN： 1465-3249            Impact factor:   5.414


  5 in total

1.  Undifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun starch-polycaprolactone scaffolds enhance bone regeneration: nude mice calvarial defect in vivo study.

Authors:  Pedro P Carvalho; Isabel B Leonor; Brenda J Smith; Isabel R Dias; Rui L Reis; Jeffrey M Gimble; Manuela E Gomes
Journal:  J Biomed Mater Res A       Date:  2013-10-12       Impact factor: 4.396

2.  Effects of autologous dental pulp stem cells and mineral trioxide aggregate on exposed dogs' dental pulp.

Authors:  Dalia K Hemdan; Manar A Selim; Rania A Galhom; Mohamed H El Daharawy; Hayam Y Hassan
Journal:  J Oral Biol Craniofac Res       Date:  2022-03-18

3.  The Anti-Tumor Effects of Adipose Tissue Mesenchymal Stem Cell Transduced with HSV-Tk Gene on U-87-Driven Brain Tumor.

Authors:  Suely Maymone de Melo; Simone Bittencourt; Enéas Galdini Ferrazoli; Clivandir Severino da Silva; Flavia Franco da Cunha; Flavia Helena da Silva; Roberta Sessa Stilhano; Priscila Martins Andrade Denapoli; Bianca Ferrarini Zanetti; Priscila Keiko Matsumoto Martin; Leonardo Martins Silva; Adara Aurea dos Santos; Leandra Santos Baptista; Beatriz Monteiro Longo; Sang Won Han
Journal:  PLoS One       Date:  2015-06-12       Impact factor: 3.240

4.  Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media.

Authors:  Paola Romina Amable; Marcus Vinicius Telles Teixeira; Rosana Bizon Vieira Carias; José Mauro Granjeiro; Radovan Borojevic
Journal:  PLoS One       Date:  2014-08-12       Impact factor: 3.240

Review 5.  Mesenchymal stem cells for regenerative therapy: optimization of cell preparation protocols.

Authors:  Chiho Ikebe; Ken Suzuki
Journal:  Biomed Res Int       Date:  2014-01-06       Impact factor: 3.411
  5 in total

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