Literature DB >> 35359324

Analysis of DNA Double-Stranded Breaks Using the Comet Assay in Planarians.

Paul G Barghouth1,2, Salvador Rojas1,2, Lacey R O'Dell1, Andrew M Betancourt1, Néstor J Oviedo3,4,5.   

Abstract

Comet assay provides the opportunity to detect and characterize DNA strand breaks. Cellular lysing followed by embedding in agarose slide is used to visualize under an electrical current migration patterns corresponding to DNA fragments of different sizes. Here we describe the process of detecting and characterizing DNA damage by Comet assay on planarians, which is a model organism commonly used to understand the process of whole-body regeneration, stem cell regulation, and adult tissue maintenance.
© 2022. The Author(s).

Entities:  

Keywords:  Alkaline Comet Assay; DNA damage; Double-stranded breaks; Planarian

Mesh：

Substances：

Year:  2022        PMID： 35359324     DOI： 10.1007/978-1-0716-2172-1_25

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  Detection of DNA double-strand breaks by pulsed-field gel electrophoresis.

Authors:  Yuri Kawashima; Nahomi Yamaguchi; Rie Teshima; Hisashi Narahara; Yoshio Yamaoka; Hirofumi Anai; Yoshihiro Nishida; Katsuhiro Hanada
Journal:  Genes Cells       Date:  2016-12-15       Impact factor: 1.891

2.  A simple technique for quantitation of low levels of DNA damage in individual cells.

Authors:  N P Singh; M T McCoy; R R Tice; E L Schneider
Journal:  Exp Cell Res       Date:  1988-03       Impact factor: 3.905

3.  The comet assay: a method to measure DNA damage in individual cells.

Authors:  Peggy L Olive; Judit P Banáth
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

4.  The comet-FISH technique: a tool for detection of specific DNA damage and repair.

Authors:  Alexander Rapp; Michael Hausmann; Karl Otto Greulich
Journal:  Methods Mol Biol       Date:  2005

Review 5.  Use of Comet-FISH in the study of DNA damage and repair: review.

Authors:  Michael Glei; Galina Hovhannisyan; Beatrice L Pool-Zobel
Journal:  Mutat Res       Date:  2008-01-31       Impact factor: 2.433

6.  Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells.

Authors:  O Ostling; K J Johanson
Journal:  Biochem Biophys Res Commun       Date:  1984-08-30       Impact factor: 3.575

Review 7.  The DNA damage response and cancer therapy.

Authors:  Christopher J Lord; Alan Ashworth
Journal:  Nature       Date:  2012-01-18       Impact factor: 49.962

8.  Introducing a true internal standard for the Comet assay to minimize intra- and inter-experiment variability in measures of DNA damage and repair.

Authors:  Murizal Zainol; Julia Stoute; Gabriela M Almeida; Alexander Rapp; Karen J Bowman; George D D Jones
Journal:  Nucleic Acids Res       Date:  2009-12       Impact factor: 16.971

9.  The comet assay: past, present, and future.

Authors:  Sabine A S Langie; Amaya Azqueta; Andrew R Collins
Journal:  Front Genet       Date:  2015-08-13       Impact factor: 4.599

10.  Comet-FISH with strand-specific probes reveals transcription-coupled repair of 8-oxoGuanine in human cells.

Authors:  Jia Guo; Philip C Hanawalt; Graciela Spivak
Journal:  Nucleic Acids Res       Date:  2013-06-17       Impact factor: 16.971
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