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Literature DB >> 3535283

Assessment of the in vitro growth of Plasmodium falciparum using fluorometry.

Abstract

The growth of Plasmodium falciparum in vitro was quantitatively assessed by applying fluorometry using ethidium bromide. The fluorescence intensity of parasites stained with this dye was found to parallel the uptake of 3H-hypoxanthine into nucleic acids during one growth cycle of development. The assay system can be used as a substitute of morphological and radiometric methods in drug-sensitivity tests and for the screening of antimalarials.

Entities: Chemical Species

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Year: 1986 PMID： 3535283 DOI： 10.1007/BF00925480

Source DB: PubMed Journal: Z Parasitenkd ISSN： 0044-3255

9 in total

1. Cytofluorograf detection of Plasmodium yoelii, Trypanosoma gambiense, and Trypanosoma equiperdum by laser excited fluorescence of stained rodent blood.

Authors: P R Jackson; D G Winkler; S L Kimzey; F M Fisher
Journal: J Parasitol Date: 1977-08 Impact factor: 1.276

1 in total

1. A fluorometric sensitivity test for chloroquine in Plasmodium falciparum isolates from patients.

Authors: S Waki; J Tamura; T Takagi; M Adachi; M Suzuki
Journal: Parasitol Res Date: 1987 Impact factor: 2.289

1 in total

Assessment of the in vitro growth of Plasmodium falciparum using fluorometry.

1. Cytofluorograf detection of Plasmodium yoelii, Trypanosoma gambiense, and Trypanosoma equiperdum by laser excited fluorescence of stained rodent blood.

2. In vitro cultivation of Plasmodium falciparum under aerobic atmosphere in a CO2 incubator.

3. A new technique for drug susceptibility tests for Plasmodium falciparum by ethidium bromide fluoroassay.

4. Plasmodium falciparum: automated assay of erythrocyte invasion using flow cytofluorometry.

5. Drug sensitivity of plasmodium falciparum. An in-vitro microtechnique.

6. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.

7. Human malaria parasites in continuous culture.

8. Synchronization of Plasmodium falciparum erythrocytic stages in culture.

9. Plasmodium falciparum: attenuation by irradiation.

1. A fluorometric sensitivity test for chloroquine in Plasmodium falciparum isolates from patients.