OBJECTIVES: To investigate the mutation rate of the RAS gene and its clinical significance in children with acute lymphoblastic leukemia. METHODS: A retrospective analysis was performed on the medical data of 120 children with newly diagnosed acute lymphoblastic leukemia, who were admitted to the Third Affiliated Hospital of Zhengzhou University from January 2015 to January 2020 and underwent next-generation sequencing. The clinical and molecular features were analyzed. The impact of RAS gene mutation on the overall survival rate was evaluated in these children. RESULTS: Among the 120 children, 35 (29.2%) had RAS gene mutation, 30 (25.0%) had KRAS gene mutation, and 5 (4.2%) had both NRAS and KRAS gene mutations. All NRAS mutations and 71% (25/35) of KRAS mutations were located at the 12th and 13th codons. RAS gene mutation was detected in 35 (33.3%) out of 105 children with B-lineage acute lymphoblastic leukemia, but it was not detected in those with acute T lymphocyte leukemia. Of all the children, 11 (9.2%) were lost to follow-up, and among the 109 children followed up, 16 (14.7%) died. The children with RAS gene mutation had a significantly lower 2-year overall survival rate than those without RAS gene mutation (P<0.05). The prognosis of children with RAS gene mutation combined with WT1 overexpression and WBC>50×109/L at diagnosis was worse (P<0.05). CONCLUSIONS: RAS gene mutation is commonly observed in children with B-lineage acute lymphoblastic leukemia and may have an adverse effect on prognosis.
OBJECTIVES: To investigate the mutation rate of the RAS gene and its clinical significance in children with acute lymphoblastic leukemia. METHODS: A retrospective analysis was performed on the medical data of 120 children with newly diagnosed acute lymphoblastic leukemia, who were admitted to the Third Affiliated Hospital of Zhengzhou University from January 2015 to January 2020 and underwent next-generation sequencing. The clinical and molecular features were analyzed. The impact of RAS gene mutation on the overall survival rate was evaluated in these children. RESULTS: Among the 120 children, 35 (29.2%) had RAS gene mutation, 30 (25.0%) had KRAS gene mutation, and 5 (4.2%) had both NRAS and KRAS gene mutations. All NRAS mutations and 71% (25/35) of KRAS mutations were located at the 12th and 13th codons. RAS gene mutation was detected in 35 (33.3%) out of 105 children with B-lineage acute lymphoblastic leukemia, but it was not detected in those with acute T lymphocyte leukemia. Of all the children, 11 (9.2%) were lost to follow-up, and among the 109 children followed up, 16 (14.7%) died. The children with RAS gene mutation had a significantly lower 2-year overall survival rate than those without RAS gene mutation (P<0.05). The prognosis of children with RAS gene mutation combined with WT1 overexpression and WBC>50×109/L at diagnosis was worse (P<0.05). CONCLUSIONS: RAS gene mutation is commonly observed in children with B-lineage acute lymphoblastic leukemia and may have an adverse effect on prognosis.
Authors: J L Wiemels; Y Zhang; J Chang; S Zheng; C Metayer; L Zhang; M T Smith; X Ma; S Selvin; P A Buffler; J K Wiencke Journal: Leukemia Date: 2005-03 Impact factor: 11.528
Authors: Linda Holmfeldt; Lei Wei; Ernesto Diaz-Flores; Michael Walsh; Jinghui Zhang; Li Ding; Debbie Payne-Turner; Michelle Churchman; Anna Andersson; Shann-Ching Chen; Kelly McCastlain; Jared Becksfort; Jing Ma; Gang Wu; Samir N Patel; Susan L Heatley; Letha A Phillips; Guangchun Song; John Easton; Matthew Parker; Xiang Chen; Michael Rusch; Kristy Boggs; Bhavin Vadodaria; Erin Hedlund; Christina Drenberg; Sharyn Baker; Deqing Pei; Cheng Cheng; Robert Huether; Charles Lu; Robert S Fulton; Lucinda L Fulton; Yashodhan Tabib; David J Dooling; Kerri Ochoa; Mark Minden; Ian D Lewis; L Bik To; Paula Marlton; Andrew W Roberts; Gordana Raca; Wendy Stock; Geoffrey Neale; Hans G Drexler; Ross A Dickins; David W Ellison; Sheila A Shurtleff; Ching-Hon Pui; Raul C Ribeiro; Meenakshi Devidas; Andrew J Carroll; Nyla A Heerema; Brent Wood; Michael J Borowitz; Julie M Gastier-Foster; Susana C Raimondi; Elaine R Mardis; Richard K Wilson; James R Downing; Stephen P Hunger; Mignon L Loh; Charles G Mullighan Journal: Nat Genet Date: 2013-01-20 Impact factor: 38.330
Authors: J P Perentesis; S Bhatia; E Boyle; Y Shao; X Ou Shu; M Steinbuch; H N Sather; P Gaynon; W Kiffmeyer; J Envall-Fox; L L Robison Journal: Leukemia Date: 2004-04 Impact factor: 11.528
Authors: J Y Wu; B Li; Y J Jia; P H Zhang; Z F Xu; T J Qin; S Q Qu; L J Pan; J Q Liu; X Yan; Y D Zhang; J Chen; J Y Gong; Z J Xiao Journal: Zhonghua Xue Ye Xue Za Zhi Date: 2020-12-14