Literature DB >> 35344174

Assessing Changes in Human Natural Killer Cell Metabolism Using the Seahorse Extracellular Flux Analyzer.

Javier Traba1, Olga M Antón2,3.   

Abstract

Natural killer (NK) cells are cytotoxic cells that mediate anti-tumor and anti-viral immunity. The response of NK cells to different cytokines and stimuli may involve cell survival, proliferation, and changes in their cytotoxic function. These responses will be supported by changes in cellular metabolism. Therefore, changes in NK metabolic parameters could somehow predict changes in NK cell function and cytotoxicity. In this chapter, we describe a protocol to measure NK cell metabolism in primary human NK cells by using an extracellular flux analyzer. This machine measures pH and oxygen changes in the medium and allows the study of NK cell glycolysis and mitochondrial respiration in real time with a small number of cells.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Electron transporter chain; Extracellular flux analyzer; Glycolysis; Mitochondrial respiration; Natural killer cell

Mesh：

Year:  2022        PMID： 35344174     DOI： 10.1007/978-1-0716-2160-8_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

Review 1.  Immunometabolism and natural killer cell responses.

Authors:  Katie L O'Brien; David K Finlay
Journal:  Nat Rev Immunol       Date:  2019-05       Impact factor: 53.106

Review 2.  The regulation and physiology of mitochondrial proton leak.

Authors:  Ajit S Divakaruni; Martin D Brand
Journal:  Physiology (Bethesda)       Date:  2011-06

3.  Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular flux measurements.

Authors:  Shona A Mookerjee; Akos A Gerencser; David G Nicholls; Martin D Brand
Journal:  J Biol Chem       Date:  2017-03-07       Impact factor: 5.157

Review 4.  Assessing mitochondrial dysfunction in cells.

Authors:  Martin D Brand; David G Nicholls
Journal:  Biochem J       Date:  2011-04-15       Impact factor: 3.857

Review 5.  Post-translational modifications in mitochondria: protein signaling in the powerhouse.

Authors:  Amanda R Stram; R Mark Payne
Journal:  Cell Mol Life Sci       Date:  2016-05-27       Impact factor: 9.261

6.  Analysis of Intracellular Ca(2+) Mobilization in Human NK Cell Subsets by Flow Cytometry.

Authors:  Jakob Theorell; Yenan T Bryceson
Journal:  Methods Mol Biol       Date:  2016

7.  The contributions of respiration and glycolysis to extracellular acid production.

Authors:  Shona A Mookerjee; Renata L S Goncalves; Akos A Gerencser; David G Nicholls; Martin D Brand
Journal:  Biochim Biophys Acta       Date:  2014-10-27

8.  Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements.

Authors:  Shona A Mookerjee; David G Nicholls; Martin D Brand
Journal:  PLoS One       Date:  2016-03-31       Impact factor: 3.240
  8 in total

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