Literature DB >> 3531183

Cloning and characterization of the gene encoding 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli.

M Woisetschläger, G Högenauer.   

Abstract

The cloning of the gene for Escherichia coli PL-2 2-keto-3-deoxy-D-manno-octonate 8-phosphate synthetase is reported. Positive transformants showed an increase of approximately three-fold in specific activity of the enzyme both in E. coli and in Salmonella typhimurium as host cells. A subclone containing a 1.5-kilobase PvuII fragment overproduced active enzyme. Minicell experiments that allow the detection of plasmid encoded proteins revealed an insert-coded single protein band of 34 kilodaltons.

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Year:  1986        PMID： 3531183      PMCID： PMC213473          DOI： 10.1128/jb.168.1.437-439.1986

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  11 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors:  D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

5.  Isolation of mutants conditionally blocked in the biosynthesis of the 3-deoxy-D-manno-octulosonic-acid--lipid-A part of lipopolysaccharides derived from Salmonella typhimurium.

Authors:  V Lehmann; E Rupprecht; M J Osborn
Journal:  Eur J Biochem       Date:  1977-06-01

6.  Biosynthesis of lipid A. Enzymatic incorporation of 3-deoxy-D-mannooctulosonate into a precursor of lipid A in Salmonella typhimurium.

Authors:  R S Munson; N S Rasmussen; M J Osborn
Journal:  J Biol Chem       Date:  1978-03-10       Impact factor: 5.157

7.  Purification and characterization of 3-deoxy-D-manno-octulosonate 8-phosphate synthetase from Escherichia coli.

Authors:  P H Ray
Journal:  J Bacteriol       Date:  1980-02       Impact factor: 3.490

8.  Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate).

Authors:  P D Rick; M J Osborn
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205

9.  R factor proteins synthesized in Escherichia coli minicells: incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins.

Authors:  S B Levy
Journal:  J Bacteriol       Date:  1974-12       Impact factor: 3.490

10.  Free flow electrophoresis as a tool for enrichment of mutants with temperature-dependent lethal mutations in lipid A synthesis.

Authors:  T Hansen-Hagge; V Lehmann; O Lüderitz
Journal:  Eur J Biochem       Date:  1985-04-01
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  4 in total

1.  Isolation of Salmonella typhimurium strains that utilize exogenous 3-deoxy-D-manno-octulosonate for synthesis of lipopolysaccharide.

Authors:  R C Goldman; E M Devine
Journal:  J Bacteriol       Date:  1987-11       Impact factor: 3.490

2.  The kdsA gene coding for 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase is part of an operon in Escherichia coli.

Authors:  M Woisetschläger; G Högenauer
Journal:  Mol Gen Genet       Date:  1987-05

3.  Localization of the kdsA gene with the aid of the physical map of the Escherichia coli chromosome.

Authors:  M Woisetschläger; A Hödl-Neuhofer; G Högenauer
Journal:  J Bacteriol       Date:  1988-11       Impact factor: 3.490

4.  Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.

Authors:  H Strohmaier; P Remler; W Renner; G Högenauer
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490
  4 in total

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