Purpose: Benzoyl peroxide (BPO) is an effective acne treatment and has been used as a cleanser and short contact therapy. However, data on the minimum contact time of BPO needed to kill Cutibacterium acnes are lacking. Thus, the aim of this study was to determine the minimum contact time of commonly used BPO concentrations for bactericidal effects on C. acnes. Materials and Methods: An in vitro experimental study of clinically isolated C. acnes was performed to determine the minimal inhibitory concentration (MIC) of BPO using the broth microdilution method. Subsequently, the minimum contact times of various concentrations of BPO were evaluated, and their bactericidal effects were assessed by the plate count method. Results: The median MIC of BPO was 9375 µg/mL, which did not significantly differ between antibiotic-resistant and nonresistant C. acnes. The minimum contact time of BPO with C. acnes was significantly different among the BPO concentrations. For bactericidal activity against all isolates, 1.25%, 2.5%, 5%, and 10% BPO required 60 min, 15 min, 30 sec, and 30 sec, respectively. Conclusion: BPO demonstrated bactericidal activity against both antibiotic-resistant and antibiotic-susceptible C. acnes. The in vitro contact time needed to kill C. acnes was almost immediate with 5% or more BPO, but ≤ 2.5% BPO required longer contact times for bactericidal effects.
Purpose: Benzoyl peroxide (BPO) is an effective acne treatment and has been used as a cleanser and short contact therapy. However, data on the minimum contact time of BPO needed to kill Cutibacterium acnes are lacking. Thus, the aim of this study was to determine the minimum contact time of commonly used BPO concentrations for bactericidal effects on C. acnes. Materials and Methods: An in vitro experimental study of clinically isolated C. acnes was performed to determine the minimal inhibitory concentration (MIC) of BPO using the broth microdilution method. Subsequently, the minimum contact times of various concentrations of BPO were evaluated, and their bactericidal effects were assessed by the plate count method. Results: The median MIC of BPO was 9375 µg/mL, which did not significantly differ between antibiotic-resistant and nonresistant C. acnes. The minimum contact time of BPO with C. acnes was significantly different among the BPO concentrations. For bactericidal activity against all isolates, 1.25%, 2.5%, 5%, and 10% BPO required 60 min, 15 min, 30 sec, and 30 sec, respectively. Conclusion: BPO demonstrated bactericidal activity against both antibiotic-resistant and antibiotic-susceptible C. acnes. The in vitro contact time needed to kill C. acnes was almost immediate with 5% or more BPO, but ≤ 2.5% BPO required longer contact times for bactericidal effects.
Authors: Andrea L Zaenglein; Arun L Pathy; Bethanee J Schlosser; Ali Alikhan; Hilary E Baldwin; Diane S Berson; Whitney P Bowe; Emmy M Graber; Julie C Harper; Sewon Kang; Jonette E Keri; James J Leyden; Rachel V Reynolds; Nanette B Silverberg; Linda F Stein Gold; Megha M Tollefson; Jonathan S Weiss; Nancy C Dolan; Andrew A Sagan; Mackenzie Stern; Kevin M Boyer; Reva Bhushan Journal: J Am Acad Dermatol Date: 2016-02-17 Impact factor: 11.527
Authors: Diane M Thiboutot; Brigitte Dréno; Abdullah Abanmi; Andrew F Alexis; Elena Araviiskaia; Maria Isabel Barona Cabal; Vincenzo Bettoli; Flordeliz Casintahan; Steven Chow; Adilson da Costa; Tam El Ouazzani; Chee-Leok Goh; Harald P M Gollnick; Minerva Gomez; Nobukazu Hayashi; Maria Isabel Herane; Juan Honeyman; Sewon Kang; Lajos Kemeny; Raj Kubba; Julien Lambert; Alison M Layton; James J Leyden; Jose Luis López-Estebaranz; Nopadon Noppakun; Falk Ochsendorf; Cristina Oprica; Beatriz Orozco; Montserrat Perez; Jaime Piquero-Martin; Jo-Ann See; Dae Hun Suh; Jerry Tan; Vicente Torres Lozada; Patricia Troielli; Leihong Flora Xiang Journal: J Am Acad Dermatol Date: 2017-11-08 Impact factor: 11.527
Authors: J Pannu; A McCarthy; A Martin; T Hamouda; S Ciotti; L Ma; J Sutcliffe; J R Baker Journal: Antimicrob Agents Chemother Date: 2011-07-11 Impact factor: 5.191