Literature DB >> 35294758

GFP-LC3 High-Content Assay for Screening Autophagy Modulators.

Li Zhang1, Jinghua Zhao1, Wen-Xing Ding2, Menghang Xia3.   

Abstract

Autophagy plays an important role in maintaining cellular homeostasis. Defects in autophagy have been linked to various human diseases, such as cancer, neurodegenerative diseases, and cardiovascular diseases. Therefore, it is useful to develop an assay that can measure the functions of autophagy and also be used to identify autophagy modulators by screening a large number of compounds. This chapter describes a cell-based high content green fluorescent protein (GFP)-LC3 assay using mouse embryonic fibroblasts (MEF) stably expressing GFP-LC3.
© 2022. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

Entities:  

Keywords:  Autophagy; GFP-LC3 cell line; High-content assay

Mesh：

Substances：

Year:  2022        PMID： 35294758      PMCID： PMC9438799          DOI： 10.1007/978-1-0716-2213-1_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

Review 1.  Autophagy: renovation of cells and tissues.

Authors:  Noboru Mizushima; Masaaki Komatsu
Journal:  Cell       Date:  2011-11-11       Impact factor: 41.582

Review 2.  Choose Delicately and Reuse Adequately: The Newly Revealed Process of Autophagy.

Authors:  Satoru Kobayashi
Journal:  Biol Pharm Bull       Date:  2015       Impact factor: 2.233

Review 3.  Autophagy revisited: a conversation with Christian de Duve.

Authors:  Daniel J Klionsky
Journal:  Autophagy       Date:  2008-06-09       Impact factor: 16.016

4.  A cell-based quantitative high-throughput image screening identified novel autophagy modulators.

Authors:  Yuan Li; Steven McGreal; Jean Zhao; Ruili Huang; Yan Zhou; Hua Zhong; Menghang Xia; Wen-Xing Ding
Journal:  Pharmacol Res       Date:  2016-05-07       Impact factor: 7.658

Review 5.  Targeting autophagy in cancer.

Authors:  Jean M Mulcahy Levy; Christina G Towers; Andrew Thorburn
Journal:  Nat Rev Cancer       Date:  2017-07-28       Impact factor: 60.716

6.  Methods in mammalian autophagy research.

Authors:  Noboru Mizushima; Tamotsu Yoshimori; Beth Levine
Journal:  Cell       Date:  2010-02-05       Impact factor: 41.582

7.  Dissecting the dynamic turnover of GFP-LC3 in the autolysosome.

Authors:  Hong-Min Ni; Abigail Bockus; Ann L Wozniak; Kellyann Jones; Steven Weinman; Xiao-Ming Yin; Wen-Xing Ding
Journal:  Autophagy       Date:  2011-02-01       Impact factor: 16.016

Review 8.  Autophagy and aging.

Authors:  David C Rubinsztein; Guillermo Mariño; Guido Kroemer
Journal:  Cell       Date:  2011-09-02       Impact factor: 41.582

9.  Biological activity-based modeling identifies antiviral leads against SARS-CoV-2.

Authors:  Ruili Huang; Miao Xu; Hu Zhu; Catherine Z Chen; Wei Zhu; Emily M Lee; Shihua He; Li Zhang; Jinghua Zhao; Khalida Shamim; Danielle Bougie; Wenwei Huang; Menghang Xia; Mathew D Hall; Donald Lo; Anton Simeonov; Christopher P Austin; Xiangguo Qiu; Hengli Tang; Wei Zheng
Journal:  Nat Biotechnol       Date:  2021-02-23       Impact factor: 54.908

Review 10.  Autophagy Induction as a Therapeutic Strategy for Neurodegenerative Diseases.

Authors:  Alvin Djajadikerta; Swati Keshri; Mariana Pavel; Ryan Prestil; Laura Ryan; David C Rubinsztein
Journal:  J Mol Biol       Date:  2019-12-27       Impact factor: 5.469
  10 in total

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