Literature DB >> 35290638

Characterization of DNA-PK-Bound End Fragments Using GLASS-ChIP.

Rajashree A Deshpande1, Tanya T Paull2.   

Abstract

Endonucleolytic cleavage of DNA ends by the human Mre11-Rad50-Nbs1 (MRN) complex occurs in a manner that is promoted by DNA-dependent protein kinase (DNA-PK). A method is described to isolate DNA-PK-bound fragments released from chromatin in human cells using a modified Gentle Lysis and Size Selection chromatin immunoprecipitation (GLASS-ChIP) protocol. This method, combined with real-time PCR or next-generation sequencing, can identify sites of MRN endonucleolytic cutting adjacent to DNA-PK binding sites in human cells.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  DNA repair; DNA-PK; Double-strand breaks; MRN complex

Mesh：

Substances：

Year:  2022        PMID： 35290638     DOI： 10.1007/978-1-0716-2063-2_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

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8.  DNA-dependent protein kinase promotes DNA end processing by MRN and CtIP.

Authors:  Rajashree A Deshpande; Logan R Myler; Michael M Soniat; Nodar Makharashvili; Linda Lee; Susan P Lees-Miller; Ilya J Finkelstein; Tanya T Paull
Journal:  Sci Adv       Date:  2020-01-08       Impact factor: 14.136

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10.  Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells.

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