| Literature DB >> 35290635 |
Rebecca Rodell1, Ning Tsao1, Adit Ganguly1, Nima Mosammaparast2.
Abstract
Physiological and chemically induced modifications to nucleosides are common in both DNA and RNA. Physiological forms of these modifications play critical roles in gene expression, yet aberrant marks, if left unrepaired, may be associated with increased genome instability. Due to the low prevalence of these marks in most samples of interest, a highly sensitive method is needed for their detection and quantitation. High-performance liquid chromatography, coupled to mass spectrometry (HPLC-MS), provides this high degree of sensitivity while also being adaptable to nearly any modified nucleoside of interest and still maintaining exquisite specificity. In this chapter, we demonstrate how to use HPLC-MS to analyze the catalytic activity of a nucleic acid demethylase, to quantify the prevalence of N6-methyladenosine from RNA, and to determine the kinetics of alkylation damage repair.Entities:
Keywords: AlkB; Epitranscriptomics; Mass spectrometry; Methylation; Nucleoside
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Year: 2022 PMID： 35290635 PMCID： PMC9426999 DOI： 10.1007/978-1-0716-2063-2_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745