| Literature DB >> 35280577 |
Saad Alkahtani1, Md Saquib Hasnain2, Hamzah Algamdy1, Nada H Aljarba3, Abdullah AlKahtane1.
Abstract
Through the boost of the natural medicinal market, individuals began to use a variety of organic materials in the marketed herbal preparation. Lagerstroemia speciosa (LS) leaves are known as banaba. People have been using a decoction of LS leaves as antidiabetic. The study aimed to investigate the acute and sub-acute oral toxicity of LS in Sprague-Dawley rats. The acute toxicity was determined by a single oral dose of LS (2000 mg/kg). Therein animal behaviour and mortality rate were observed for 14 days. The LS (200 mg/kg) was given for 28 days daily in the sub-acute study. The body weight, organ weight, food, water intake, biochemical, haematological parameters, and histopathology were studied. The findings of this study showed no mortality or morbidity was found in acute and sub-acute toxicity studies in rats. Additionally, no significant variations were found in the respective weight of organ, haematological and biochemical parameters of treated groups with reference to the control group. Moreover, no visible histological changes were detected in the liver of treated groups with reference to the control. In conclusion, the oral administration of LS did not fabricate any major toxic effect in rats. No toxic consequences were reported during acute and sub-acute toxicity investigations. Overall, LS is a safe, natural bio-actives as studied. Further investigations of cytotoxicity and genotoxicity of the above drug(s) or their combinations may be executed for appreciative safety.Entities:
Keywords: ALP, Alkaline phosphatase; ALT, Alanine aminotransferase; AST, Aspartate aminotransferase; Biochemical investigations; FBG, Fasting blood glucose; GSH, Reduced glutathione; Haematology; Histopathology; LS, Lagerstroemia speciosa; MDA, Malondialdehyde; ROS, Reactive oxygen species; SOD, Superoxide dismutase; Toxicity
Year: 2021 PMID: 35280577 PMCID: PMC8913382 DOI: 10.1016/j.sjbs.2021.11.005
Source DB: PubMed Journal: Saudi J Biol Sci ISSN: 2213-7106 Impact factor: 4.219
Fig. 1Effect of LS on (A) body weight, (B) fasting blood glucose level. Data are represented as mean ± SEM.
Effect of LS on food and water consumptionin SD rats.
| Parameters | Control | LS | |
|---|---|---|---|
| Sub- toxicity | Food intake (g/day) | 21.08 ± 0.56 | 21.71 ± 0.53 |
| Water intake (ml/day) | 67.47 ± 1.74 | 71.45 ± 2.08 | |
All values were presented as Mean ± SEM.
Fig. 2Effect of LS on different organ weights of the body of rat. Data are represented as mean ± SEM.
Fig. 3Effect of LS on (A) ROS, (B) MDA level, (C) GSH level, and (D) SOD activity. Data are represented as mean ± SEM.
Effect of LS on haematological parameters in SD rats.
| Parameters | Control | LS | |
|---|---|---|---|
| Sub-acute toxicity | Erythrocytes (106/µL) | 6.67 ± 0.10 | 6.46 ± 0.23 |
| Leukocytes (cells/mm3) | 7760 ± 360 | 7840 ± 258.07 | |
| Platelets (Lac cells/mm3) | 2.78 ± 0.21 | 2.79 ± 0.16 | |
| Hematocrit (%) | 37.02 ± 0.79 | 36.92 ± 1.23 | |
| Hemoglobin (g/dL) | 12.78 ± 0.21 | 12.18 ± 0.39 | |
All values were presented as Mean ± SEM.
Effect of LSonbiochemical parameters in SD rats.
| Parameters | Control | LS | |
|---|---|---|---|
| Sub-acute toxicity | Total bilirubin (mg/dl) | 0.72 ± 0.06 | 0.72 ± 0.05 |
| Bilirubin direct (mg/dl) | 0.28 ± 0.03 | 0.27 ± 0.04 | |
| Serum Cr (mg/dl) | 0.74 ± 0.02 | 0.77 ± 0.06 | |
| AST (IU/L) | 36.26 ± 0.44 | 35.60 ± 0.36 | |
| ALT (IU/L) | 31.93 ± 0.36 | 31.20 ± 0.47 | |
| ALP (IU/L) | 38.94 ± 0.44 | 38.28 ± 0.36 | |
All values were presented as Mean ± SEM.
Fig. 4Histopathological analysis of liver tissue on administration of LS in sub-acute toxicity.