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Literature DB >> 35258836

Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis Via Protoplast Transfection.

Adam Sychla^1,2,3, Juan Armando Casas-Mollano^1,2,3, Matthew H Zinselmeier^1,2,3, Michael Smanski^4,5,6.

Abstract

Recent advances in DNA synthesis and assembly allow for genetic constructs to be designed and constructed in high throughput. Characterizing large numbers of variant genetic designs is not feasible with low-throughput and time-consuming plant transformation workflows. Protoplast transformation offers a rapid, high-throughput compatible alternative for testing genetic constructs in plant-relevant molecular environments. Here, we describe a protocol for protoplast transformation using a recent experiment in genetic optimization of dCas9-based programmable transcription activators as an example.

Entities: Chemical

Keywords: High throughput; Monocot; Protoplasts; Setaria viridis; Transcription; Transfection; dCas9

Mesh：

Year: 2022 PMID： 35258836 DOI： 10.1007/978-1-0716-2164-6_16

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

7 in total

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Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis Via Protoplast Transfection.

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Review 5. Production and metabolic engineering of bioactive substances in plant hairy root culture.

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