Literature DB >> 35237964

Monocyte Adhesion Assays for Detecting Endothelial Cell Activation in Vascular Inflammation and Atherosclerosis.

Fatma Saaoud1, Stephanie Yu2, Keman Xu1, Charles Drummer1, Ying Shao1, Yu Sun1, Yifan Lu1, Jianxin Sun3, Jun Yu4, Xiaohua Jiang1,4, Hong Wang4, Xiaofeng Yang5,6,7.   

Abstract

Monocyte adhesion assay, a fluorescence-based method, enables the detection and quantification of monocyte adhesion to endothelial cell (EC) monolayers in vitro and measures EC activation. We describe in this chapter a monocyte adhesion assay based on two published papers from our laboratory that can be effectively used in studying the mechanisms of both pro- and anti-inflammatory cytokines in EC activation. Endothelial cell monolayers are cultured and treated with desired drug, cytokines, or other stimuli and incubated with fluorescently labeled monocytes.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Adhesion molecules; Atherosclerosis; Endothelial cell activation; Inflammation; Monocyte adhesion assay; Monocytes

Mesh：

Year:  2022        PMID： 35237964      PMCID： PMC9219302          DOI： 10.1007/978-1-0716-1924-7_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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Journal:  AIDS Rev       Date:  2015 Oct-Dec       Impact factor: 2.500

4.  Interleukin-17A Promotes Aortic Endothelial Cell Activation via Transcriptionally and Post-translationally Activating p38 Mitogen-activated Protein Kinase (MAPK) Pathway.

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Journal:  J Biol Chem       Date:  2016-01-05       Impact factor: 5.157

5.  Lysophospholipids induce innate immune transdifferentiation of endothelial cells, resulting in prolonged endothelial activation.

Authors:  Xinyuan Li; Luqiao Wang; Pu Fang; Yu Sun; Xiaohua Jiang; Hong Wang; Xiao-Feng Yang
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6.  Vascular endothelial cells mediate mechanical stimulation-induced enhancement of endothelin hyperalgesia via activation of P2X2/3 receptors on nociceptors.

Authors:  Elizabeth K Joseph; Paul G Green; Oliver Bogen; Pedro Alvarez; Jon D Levine
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9.  Global, Regional, and National Burden of Cardiovascular Diseases for 10 Causes, 1990 to 2015.

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Journal:  J Am Coll Cardiol       Date:  2017-05-17       Impact factor: 24.094

Review 10.  Role of Histamine in Modulating the Immune Response and Inflammation.

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2.  Editorial: Insights in cardiovascular therapeutics: 2021 - cell death, cardiovascular injuries, and novel targets of cardiovascular therapeutics.

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  2 in total

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