Isolation and Culturing of Primary Mouse and Human Macrophages.

Macrophages are mature immune cells involved in the defense of the organism. Since their discovery, the main function attributed to macrophages has been phagocytosis. However, in recent years, several new functions such as angiogenesis, tissue remodeling, clearance of apoptotic cells, pro- and anti-inflammatory properties and tumor growth have been attributed to macrophages. To perform such varied functions, macrophages acquire specific phenotypes in response to external signals. The possibility of replicating these phenotypes in vitro represents a cutting-edge tool to understand potential macrophage functions in vivo. This chapter outlines protocols used to isolate and culture murine bone marrow-derived and human monocyte-derived macrophages. Furthermore, macrophage polarization processes into different phenotypes, with special relevance to atherosclerosis, are indicated.