Literature DB >> 3522627

Enzyme-linked immunosorbent assay for shigella toxin.

A Donohue-Rolfe, M A Kelley, M Bennish, G T Keusch.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of shigella toxin. For the assay, a mouse monoclonal antibody against the B subunit of the toxin and a rabbit polyclonal antibody against the holotoxin were employed. The monoclonal antibody was used to coat wells of a microtiter plate, and the polyclonal antibody preparation was used as the detecting antibody. The amount of bound polyclonal antibody was determined by using a goat anti-rabbit immunoglobulin G-alkaline phosphatase conjugate and substrate. The ELISA was able to detect as little as 12 pg (0.06 ng/ml) of shigella toxin. The assay was specific for shigella toxin, not detecting a variety of other bacterial enterotoxins and lethal toxins. The ELISA values correlated well with cytotoxin activity during toxin purification. Shigella toxin was detected by ELISA and by immunoblot analysis in human fecal specimens from persons with S. dysenteriae infections, demonstrating that this toxin is produced in vivo.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3522627      PMCID： PMC268833          DOI： 10.1128/jcm.24.1.65-68.1986

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus.

Authors:  R H Yolken; P J Stopa
Journal:  J Clin Microbiol       Date:  1979-11       Impact factor: 5.948

2.  Restriction in vivo. III. General effects of glucosylation and restriction on phage T4 gene expression and replication.

Authors:  K Dharmalingam; E B Goldberg
Journal:  Virology       Date:  1979-07-30       Impact factor: 3.616

3.  Serum enterotoxin-neutralizing antibody in human shigellosis.

Authors:  G T Keusch; M Jacewicz
Journal:  Nat New Biol       Date:  1973-01-03

4.  The pathogenesis of shigella diarrhea. II. Enterotoxin-induced acute enteritis in the rabbit ileum.

Authors:  G T Keusch; G F Grady; A Takeuchi; H Sprinz
Journal:  J Infect Dis       Date:  1972-07       Impact factor: 5.226

5.  Quantitative microassay in cell culture for enterotoxin of Shigella dysenteriae.

Authors:  G T Keusch; M Jacewicz; S Z Hirschman
Journal:  J Infect Dis       Date:  1972-05       Impact factor: 5.226

6.  Inhibition of protein synthesis in intact HeLa cells by Shigella dysenteriae 1 toxin.

Authors:  J E Brown; S W Rothman; B P Doctor
Journal:  Infect Immun       Date:  1980-07       Impact factor: 3.441

7.  Effect of Shigella enterotoxin on electrolyte transport in rabbit ileum.

Authors:  M Donowitz; G T Keusch; H J Binder
Journal:  Gastroenterology       Date:  1975-12       Impact factor: 22.682

8.  Biological properties of Shigella flexneri 2A toxin and its serological relationship to Shigella dysenteriae 1 toxin.

Authors:  A D O'Brien; M R Thompson; P Gemski; B P Doctor; S B Formal
Journal:  Infect Immun       Date:  1977-03       Impact factor: 3.441

9.  The pathogenesis of Shigella diarrhea. I. Enterotoxin production by Shigella dysenteriae I.

Authors:  G T Keusch; G F Grady; L J Mata; J McIver
Journal:  J Clin Invest       Date:  1972-05       Impact factor: 14.808

10.  Stool desorbing activity: a possible cause of false-positive reactions in competitive enzyme immunoassays.

Authors:  M Hanvanich; R Viscidi; B E Laughon; J G Bartlett; R H Yolken
Journal:  J Clin Microbiol       Date:  1985-02       Impact factor: 5.948
View more
  19 in total

1.  Shiga toxins 1 and 2 translocate differently across polarized intestinal epithelial cells.

Authors:  B P Hurley; M Jacewicz; C M Thorpe; L L Lincicome; A J King; G T Keusch; D W Acheson
Journal:  Infect Immun       Date:  1999-12       Impact factor: 3.441

2.  A system for production and rapid purification of large amounts of the Shiga toxin/Shiga-like toxin I B subunit.

Authors:  S B Calderwood; D W Acheson; M B Goldberg; S A Boyko; A Donohue-Rolfe
Journal:  Infect Immun       Date:  1990-09       Impact factor: 3.441

3.  Detection and production of verotoxin 1 of Escherichia coli O157:H7 in food.

Authors:  R D Weeratna; M P Doyle
Journal:  Appl Environ Microbiol       Date:  1991-10       Impact factor: 4.792

4.  Isolation of Shiga toxin-resistant Vero cells and their use for easy identification of the toxin.

Authors:  U Kongmuang; T Honda; T Miwatani
Journal:  Infect Immun       Date:  1988-09       Impact factor: 3.441

5.  Purification of Shiga toxin and Shiga-like toxins I and II by receptor analog affinity chromatography with immobilized P1 glycoprotein and production of cross-reactive monoclonal antibodies.

Authors:  A Donohue-Rolfe; D W Acheson; A V Kane; G T Keusch
Journal:  Infect Immun       Date:  1989-12       Impact factor: 3.441

6.  Sensitive receptor-specified enzyme-linked immunosorbent assay for Escherichia coli verocytotoxin.

Authors:  M Basta; M Karmali; C Lingwood
Journal:  J Clin Microbiol       Date:  1989-07       Impact factor: 5.948

Review 7.  Shiga and Shiga-like toxins.

Authors:  A D O'Brien; R K Holmes
Journal:  Microbiol Rev       Date:  1987-06

8.  Enzyme-linked immunosorbent assay to detect Shiga toxin of Shigella dysenteriae and related toxins.

Authors:  U Kongmuang; T Honda; T Miwatani
Journal:  J Clin Microbiol       Date:  1987-01       Impact factor: 5.948

9.  Rapid method to detect shiga toxin and shiga-like toxin I based on binding to globotriosyl ceramide (Gb3), their natural receptor.

Authors:  S Ashkenazi; T G Cleary
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

10.  New immuno-PCR assay for detection of low concentrations of shiga toxin 2 and its variants.

Authors:  Wenlan Zhang; Martina Bielaszewska; Matthias Pulz; Karsten Becker; Alexander W Friedrich; Helge Karch; Thorsten Kuczius
Journal:  J Clin Microbiol       Date:  2008-02-13       Impact factor: 5.948
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.