Literature DB >> 3522624

Evaluation and optimization of the DNA filter assay for direct detection of enterotoxigenic Escherichia coli in the presence of stool coliforms.

W C Yam, M L Lung, M H Ng.   

Abstract

Direct detection of enterotoxigenic Escherichia coli (ETEC) in mixed cultures by DNA filter assay was affected by the presence of other bacteria in the cultures. By shortening incubation to 6 h, bacterial overgrowth was minimized, allowing direct detection of ETEC in stools or mixed cultures initially containing 0.2 to 3% ETEC.

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Year:  1986        PMID： 3522624      PMCID： PMC268854          DOI： 10.1128/jcm.24.1.149-151.1986

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu.

Authors:  A G Dean; Y C Ching; R G Williams; L B Harden
Journal:  J Infect Dis       Date:  1972-04       Impact factor: 5.226

2.  DNA hybridization technique for the detection of Neisseria gonorrhoeae in men with urethritis.

Authors:  P A Totten; K K Holmes; H H Handsfield; J S Knapp; P L Perine; S Falkow
Journal:  J Infect Dis       Date:  1983-09       Impact factor: 5.226

3.  Further evaluation of the Biken test (modified Elek test) for detection of enterotoxigenic Escherichia coli producing heat-labile enterotoxin and application of the test to sampling of heat-stable enterotoxin.

Authors:  T Honda; M Arita; Y Takeda; T Miwatani
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948

4.  Detection of enterotoxigenic Escherichia coli in water by filter hybridization with three enterotoxin gene probes.

Authors:  P Echeverria; J Seriwatana; O Chityothin; W Chaicumpa; C Tirapat
Journal:  J Clin Microbiol       Date:  1982-12       Impact factor: 5.948

5.  Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.

Authors:  S L Moseley; I Huq; A R Alim; M So; M Samadpour-Motalebi; S Falkow
Journal:  J Infect Dis       Date:  1980-12       Impact factor: 5.226

6.  Identification of enterotoxigenic Escherichia coli in patients with diarrhea in Asia with three enterotoxin gene probes.

Authors:  J Seriwatana; P Echeverria; J Escamilla; R Glass; I Huq; R Rockhill; B J Stoll
Journal:  Infect Immun       Date:  1983-10       Impact factor: 3.441

7.  Identification of enterotoxigenic Escherichia coli by colony hybridization using three enterotoxin gene probes.

Authors:  S L Moseley; P Echeverria; J Seriwatana; C Tirapat; W Chaicumpa; T Sakuldaipeara; S Falkow
Journal:  J Infect Dis       Date:  1982-06       Impact factor: 5.226

8.  Identification by DNA hybridization of enterotoxigenic Escherichia coli in a longitudinal study of villages in Thailand.

Authors:  P Echeverria; J Seriwatana; D N Taylor; C Tirapat; W Chaicumpa; B Rowe
Journal:  J Infect Dis       Date:  1985-01       Impact factor: 5.226
  8 in total
  4 in total

1.  New developments in medical microbiology: computer-assisted diagnosis and automated instruments.

Authors:  W Heizmann; A Pickert; T Klöss; H Werner
Journal:  Infection       Date:  1988       Impact factor: 3.553

2.  Escherichia coli associated with childhood diarrheas.

Authors:  W C Yam; M L Lung; C Y Yeung; J S Tam; M H Ng
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

3.  Clonal origin, restricted natural distribution, and conservation of virulence factors in isolates of enterotoxigenic Escherichia coli serogroup O126.

Authors:  W C Yam; M L Lung; M H Ng
Journal:  J Clin Microbiol       Date:  1988-08       Impact factor: 5.948

4.  Evaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxin.

Authors:  W C Yam; M L Lung; M H Ng
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948
  4 in total

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