Literature DB >> 1401032

Evaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxin.

W C Yam1, M L Lung, M H Ng.   

Abstract

The effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the corresponding enteric pathogens in Southeast Asia. By selection of the appropriate culture media, the sensitivity of the assay was improved from 90.6% (for the detection of heat-labile toxin) and 75% (for the detection of cholera toxin) to 100%, and the results were confirmed with bioassays and DNA hybridization assays for both clinical and environmental isolates.

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Year:  1992        PMID： 1401032      PMCID： PMC265542          DOI： 10.1128/jcm.30.9.2518-2520.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Cholera infection and toxin in the rabbit ileal loop.

Authors:  W Burrows; G M Musteikis
Journal:  J Infect Dis       Date:  1966-04       Impact factor: 5.226

2.  Escherichia coli associated with childhood diarrheas.

Authors:  W C Yam; M L Lung; C Y Yeung; J S Tam; M H Ng
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

3.  Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli.

Authors:  T Ito; S Kuwahara; T Yokota
Journal:  J Clin Microbiol       Date:  1983-01       Impact factor: 5.948

4.  Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.

Authors:  T Honda; S Taga; Y Takeda; T Miwatani
Journal:  J Clin Microbiol       Date:  1981-01       Impact factor: 5.948

5.  Rapid GM1-enzyme-linked immunosorbent assay with visual reading for identification of Escherichia coli heat-labile enterotoxin.

Authors:  A M Svennerholm; G Wiklund
Journal:  J Clin Microbiol       Date:  1983-04       Impact factor: 5.948

6.  Rapid detection by a coagglutination test of heat-labile enterotoxin in cell lysates from blood agar-grown Escherichia coli.

Authors:  B Rönnberg; T Wadström
Journal:  J Clin Microbiol       Date:  1983-06       Impact factor: 5.948

7.  Nucleotide sequence analysis of the A2 and B subunits of Vibrio cholerae enterotoxin.

Authors:  H Lockman; J B Kaper
Journal:  J Biol Chem       Date:  1983-11-25       Impact factor: 5.157

8.  Evaluation of a reversed passive latex agglutination test for detection of Escherichia coli heat-labile toxin in culture supernatants.

Authors:  S M Scotland; R H Flomen; B Rowe
Journal:  J Clin Microbiol       Date:  1989-02       Impact factor: 5.948

Review 9.  Escherichia coli diarrhoea.

Authors:  R J Gross; B Rowe
Journal:  J Hyg (Lond)       Date:  1985-12

10.  Rapid visual detection of Escherichia coli and Vibrio cholerae Heat-labile enterotoxins by nitrocellulose enzyme-linked immunosorbent assay.

Authors:  L Beutin; L Bode; T Richter; G Peltre; R Stephan
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948
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  8 in total

1.  One-step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples.

Authors:  F Nato; A Boutonnier; M Rajerison; P Grosjean; S Dartevelle; A Guénolé; N A Bhuiyan; D A Sack; G B Nair; J M Fournier; S Chanteau
Journal:  Clin Diagn Lab Immunol       Date:  2003-05

Review 2.  Cholera.

Authors:  Jason B Harris; Regina C LaRocque; Firdausi Qadri; Edward T Ryan; Stephen B Calderwood
Journal:  Lancet       Date:  2012-06-30       Impact factor: 79.321

3.  Use of rMPB70 protein and ESAT-6 peptide as antigens for comparison of the enzyme-linked immunosorbent, immunochromatographic, and latex bead agglutination assays for serodiagnosis of bovine tuberculosis.

Authors:  Hye Cheong Koo; Yong Ho Park; Jongsam Ahn; W Ray Waters; Mitch V Palmer; Mary Jo Hamilton; George Barrington; Abdelaziz A Mosaad; Kun Taek Park; Woo Kyung Jung; In Yeong Hwang; Sang-Nae Cho; Sang Jae Shin; William C Davis
Journal:  J Clin Microbiol       Date:  2005-09       Impact factor: 5.948

4.  Detection of RTX toxin gene in Vibrio cholerae by PCR.

Authors:  K H Chow; T K Ng; K Y Yuen; W C Yam
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

5.  New latex bead agglutination assay for differential diagnosis of cattle infected with Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis.

Authors:  Hye Cheong Koo; Yong Ho Park; Jongsam Ahn; W Ray Waters; Mary Jo Hamilton; George Barrington; Abdelaziz A Mosaad; Mitch V Palmer; Sang Shin; William C Davis
Journal:  Clin Diagn Lab Immunol       Date:  2004-11

6.  Quantitative detection of Vibrio cholera toxin by real-time and dynamic cytotoxicity monitoring.

Authors:  Dazhi Jin; Yun Luo; Min Zheng; Haijing Li; Jing Zhang; Melinda Stampfl; Xiao Xu; Gangqiang Ding; Yanjun Zhang; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2013-09-18       Impact factor: 5.948

Review 7.  Changing facades of Vibrio cholerae: An enigma in the epidemiology of cholera.

Authors:  N Lekshmi; Iype Joseph; T Ramamurthy; Sabu Thomas
Journal:  Indian J Med Res       Date:  2018-02       Impact factor: 2.375

Review 8.  Review of two decades of cholera diagnostics--how far have we really come?

Authors:  Michal H Dick; Martine Guillerm; Francis Moussy; Claire-Lise Chaignat
Journal:  PLoS Negl Trop Dis       Date:  2012-10-11
  8 in total

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