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Literature DB >> 35226025

Conjugation of RNA via 2'-OH acylation: Mechanisms determining nucleotide reactivity.

Abstract

The acylation reactivity of RNA 2'-OH groups has proven broadly useful for labeling and mapping RNA. Here we perform kinetics studies to test the mechanisms governing this reaction, and we find strong steric and inductive effects modulating reactivity. The results shed light on new strategies for improved conjugation and mapping.

Entities: Chemical

Mesh：

Substances：
Nucleotides
RNA

Year: 2022 PMID： 35226025 PMCID： PMC9211027 DOI： 10.1039/d2cc00660j

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.065

34 in total

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Journal: Proc Natl Acad Sci U S A Date: 2001-02-13 Impact factor: 11.205

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Authors: Edward J Merino; Kevin A Wilkinson; Jennifer L Coughlan; Kevin M Weeks
Journal: J Am Chem Soc Date: 2005-03-30 Impact factor: 15.419

Review 3. RNA labeling, conjugation and ligation.

Authors: Eduardo Paredes; Molly Evans; Subha R Das
Journal: Methods Date: 2011-02-24 Impact factor: 3.608

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Journal: J Am Chem Soc Date: 2012-07-24 Impact factor: 15.419

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Journal: Biokhimiia Date: 1965 Nov-Dec

6. Acylation probing of "generic" RNA libraries reveals critical influence of loop constraints on reactivity.

Authors: Lu Xiao; Linglan Fang; Eric T Kool
Journal: Cell Chem Biol Date: 2022-06-02 Impact factor: 9.039

7. Crystal structure of an RNA helix recognized by a zinc-finger protein: an 18-bp duplex at 1.6 A resolution.

Authors: Susana Lima; Jayne Hildenbrand; Andrei Korostelev; Stanley Hattman; Hong Li
Journal: RNA Date: 2002-07 Impact factor: 4.942

8. Stacking Free Energies of All DNA and RNA Nucleoside Pairs and Dinucleoside-Monophosphates Computed Using Recently Revised AMBER Parameters and Compared with Experiment.

Authors: Reid F Brown; Casey T Andrews; Adrian H Elcock
Journal: J Chem Theory Comput Date: 2015-04-07 Impact factor: 6.006