Literature DB >> 35212956

Reconstitution System of NLRP3 Inflammasome in HEK293T Cells.

Sheng Chen1,2, Zhexu Chi1, Di Wang3.   

Abstract

NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3) inflammasome is a cytosolic multimeric protein complex that plays key roles in the host innate immune response to both pathogenic and sterile insults. Here we describe a comprehensive guide to study NLRP3 inflammasome activation in HEK293T cell reconstitution system, which could provide direct biochemical evidence in protein interaction and posttranslational modification of the complex.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  HEK293T reconstitution system; Innate immunity; NLRP3 inflammasome

Mesh：

Substances：

Year:  2022        PMID： 35212956     DOI： 10.1007/978-1-0716-2144-8_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  Selective inhibition of the NLRP3 inflammasome by targeting to promyelocytic leukemia protein in mouse and human.

Authors:  Yu-Hsun Lo; Yu-Wen Huang; Yung-Hsuan Wu; Chi-Shan Tsai; Yu-Chuan Lin; Shu-Ting Mo; Wen-Chih Kuo; Ya-Ting Chuang; Si-Tse Jiang; Hsiu-Ming Shih; Ming-Zong Lai
Journal:  Blood       Date:  2013-02-21       Impact factor: 22.113

2.  Western Blot Techniques.

Authors:  Brianna Kim
Journal:  Methods Mol Biol       Date:  2017

3.  NLRP3 Phosphorylation Is an Essential Priming Event for Inflammasome Activation.

Authors:  Nan Song; Zhao-Shan Liu; Wen Xue; Zhao-Fang Bai; Qian-Yi Wang; Jiang Dai; Xin Liu; Yi-Jiao Huang; Hong Cai; Xiao-Yan Zhan; Qiu-Ying Han; Hongxia Wang; Yuan Chen; Hui-Yan Li; Ai-Ling Li; Xue-Min Zhang; Tao Zhou; Tao Li
Journal:  Mol Cell       Date:  2017-09-21       Impact factor: 17.970

4.  Glutathione Transferase Omega-1 Regulates NLRP3 Inflammasome Activation through NEK7 Deglutathionylation.

Authors:  Mark M Hughes; Alexander Hooftman; Stefano Angiari; Padmaja Tummala; Zbigniew Zaslona; Marah C Runtsch; Anne F McGettrick; Caroline E Sutton; Ciana Diskin; Melissa Rooke; Shuhei Takahashi; Srinivasan Sundararaj; Marco G Casarotto; Jane E Dahlstrom; Eva M Palsson-McDermott; Sinead C Corr; Kingston H G Mills; Roger J S Preston; Nouri Neamati; Yiyue Xie; Jonathan B Baell; Philip G Board; Luke A J O'Neill
Journal:  Cell Rep       Date:  2019-10-01       Impact factor: 9.423

5.  A comprehensive guide to studying inflammasome activation and cell death.

Authors:  Rebecca E Tweedell; R K Subbarao Malireddi; Thirumala-Devi Kanneganti
Journal:  Nat Protoc       Date:  2020-09-07       Impact factor: 13.491

6.  Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages.

Authors:  Chuansheng Guo; Zhexu Chi; Danlu Jiang; Ting Xu; Weiwei Yu; Zhen Wang; Sheng Chen; Li Zhang; Qianyun Liu; Xingchen Guo; Xue Zhang; Wenxin Li; Linrong Lu; Yingliang Wu; Bao-Liang Song; Di Wang
Journal:  Immunity       Date:  2018-10-23       Impact factor: 31.745

Review 7.  The NLRP3 inflammasome: molecular activation and regulation to therapeutics.

Authors:  Karen V Swanson; Meng Deng; Jenny P-Y Ting
Journal:  Nat Rev Immunol       Date:  2019-08       Impact factor: 53.106

8.  NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway.

Authors:  Iva Hafner-Bratkovič; Petra Sušjan; Duško Lainšček; Ana Tapia-Abellán; Kosta Cerović; Lucija Kadunc; Diego Angosto-Bazarra; Pablo Pelegrin; Roman Jerala
Journal:  Nat Commun       Date:  2018-12-05       Impact factor: 14.919

9.  NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component.

Authors:  Hexin Shi; Ying Wang; Xiaohong Li; Xiaoming Zhan; Miao Tang; Maggy Fina; Lijing Su; David Pratt; Chun Hui Bu; Sara Hildebrand; Stephen Lyon; Lindsay Scott; Jiexia Quan; Qihua Sun; Jamie Russell; Stephanie Arnett; Peter Jurek; Ding Chen; Vladimir V Kravchenko; John C Mathison; Eva Marie Y Moresco; Nancy L Monson; Richard J Ulevitch; Bruce Beutler
Journal:  Nat Immunol       Date:  2015-12-07       Impact factor: 25.606
  9 in total
  1 in total

1.  The Inflammasome Activity of NLRP3 Is Independent of NEK7 in HEK293 Cells Co-Expressing ASC.

Authors:  Dominik Alexander Machtens; Ian Philipp Bresch; Jan Eberhage; Thomas Frank Reubold; Susanne Eschenburg
Journal:  Int J Mol Sci       Date:  2022-09-07       Impact factor: 6.208
  1 in total

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