Literature DB >> 3520567

lac Repressor blocks transcribing RNA polymerase and terminates transcription.

U Deuschle, R Gentz, H Bujard.   

Abstract

Operator sequences are essential elements in many negatively controlled operons. By binding repressors, they prevent the formation of active complexes between RNA polymerase and promoters. Here we show that the Escherichia coli lac operator-repressor complex also efficiently interrupts ongoing transcription. This observation suggests a mechanism of action for operators located distal to promoter sequences.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3520567      PMCID： PMC323685          DOI： 10.1073/pnas.83.12.4134

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  16 in total

1.  Genetic fusions defining trp and lac operon regulatory elements.

Authors:  D H Mitchell; W S Reznikoff; J R Beckwith
Journal:  J Mol Biol       Date:  1975-04-15       Impact factor: 5.469

2.  Modulation of the two promoters of the galactose operon of Escherichia coli.

Authors:  S Adhya; W Miller
Journal:  Nature       Date:  1979-06-07       Impact factor: 49.962

3.  Ribonucleic acid isolated by cesium chloride centrifugation.

Authors:  V Glisin; R Crkvenjakov; C Byus
Journal:  Biochemistry       Date:  1974-06-04       Impact factor: 3.162

Review 4.  Cyclic AMP receptor protein: role in transcription activation.

Authors:  B de Crombrugghe; S Busby; H Buc
Journal:  Science       Date:  1984-05-25       Impact factor: 47.728

5.  A control element within a structural gene: the gal operon of Escherichia coli.

Authors:  M H Irani; L Orosz; S Adhya
Journal:  Cell       Date:  1983-03       Impact factor: 41.582

6.  Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymerase.

Authors:  S H Shanblatt; A Revzin
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

7.  Regulation of transcription from tandem and convergent promoters.

Authors:  H Horowitz; T Platt
Journal:  Nucleic Acids Res       Date:  1982-09-25       Impact factor: 16.971

8.  Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.

Authors:  R Gentz; A Langner; A C Chang; S N Cohen; H Bujard
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

9.  Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.

Authors:  R P Gunsalus; C Yanofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

10.  Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.

Authors:  D Stueber; H Bujard
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
View more
  49 in total

1.  A DNA translocation motif in the bacterial transcription--repair coupling factor, Mfd.

Authors:  A L Chambers; A J Smith; N J Savery
Journal:  Nucleic Acids Res       Date:  2003-11-15       Impact factor: 16.971

2.  Development of a thermally regulated broad-spectrum promoter system for use in pathogenic gram-positive species.

Authors:  David A Schofield; Caroline Westwater; Brian D Hoel; Phillip A Werner; James S Norris; Michael G Schmidt
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

3.  Repression of Escherichia coli purB is by a transcriptional roadblock mechanism.

Authors:  B He; H Zalkin
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

4.  Displacement of Xenopus transcription factor IIIA from a 5S rRNA gene by a transcribing RNA polymerase.

Authors:  F E Campbell; D R Setzer
Journal:  Mol Cell Biol       Date:  1991-08       Impact factor: 4.272

5.  Transcriptional silencing by the mycobacteriophage L5 repressor.

Authors:  K L Brown; G J Sarkis; C Wadsworth; G F Hatfull
Journal:  EMBO J       Date:  1997-10-01       Impact factor: 11.598

6.  DNA sequences in gal operon override transcription elongation blocks.

Authors:  Dale E A Lewis; Natalia Komissarova; Phuoc Le; Mikhail Kashlev; Sankar Adhya
Journal:  J Mol Biol       Date:  2008-07-27       Impact factor: 5.469

7.  Characterization of elongating T7 and SP6 RNA polymerases and their response to a roadblock generated by a site-specific DNA binding protein.

Authors:  P A Pavco; D A Steege
Journal:  Nucleic Acids Res       Date:  1991-09-11       Impact factor: 16.971

8.  Inducible gene expression from African swine fever virus recombinants: analysis of the major capsid protein p72.

Authors:  R García-Escudero; G Andrés; F Almazán; E Viñuela
Journal:  J Virol       Date:  1998-04       Impact factor: 5.103

9.  Transcriptional regulation of fatty acid cis-trans isomerization in the solvent-tolerant soil bacterium, Pseudomonas putida F1.

Authors:  Tatiana Kondakova; John E Cronan
Journal:  Environ Microbiol       Date:  2019-03-12       Impact factor: 5.491

10.  Genetic evidence that the XylS regulator of the Pseudomonas TOL meta operon controls the Pm promoter through weak DNA-protein interactions.

Authors:  B Kessler; M Herrero; K N Timmis; V de Lorenzo
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.