Literature DB >> 6946440

Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.

R Gentz, A Langner, A C Chang, S N Cohen, H Bujard.   

Abstract

Downstream placement of a strong transcriptional termination signal has made possible the cloning of bacteriophage T5 promoters known to exhibit high signal strength. The cloning system constructed contains two easily assayable indicator functions whose expression is controlled by the integration of promoters and terminators, respectively. By assessing transcription within the indicator regions, the efficiency of promoters as well as termination signals can be determined in vitro and in vivo.

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Year:  1981        PMID： 6946440      PMCID： PMC320300          DOI： 10.1073/pnas.78.8.4936

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Construction of plasmids carrying the cI gene of bacteriophage lambda.

Authors:  K Backman; M Ptashne; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

2.  Termination of transcription by Escherichia coli RNA polymerase in vitro is affected by ribonucleoside triphosphate base analogs.

Authors:  N F Neff; M J Chamberlin
Journal:  J Biol Chem       Date:  1978-04-10       Impact factor: 5.157

3.  Interaction of E. coli RNA polymerase with promotors of coliphage T5: the rates of complex formation and decay and their correlation with in vitro and in vivo transcriptional activity.

Authors:  A von Gabain; H Bujard
Journal:  Mol Gen Genet       Date:  1977-12-09

4.  Isolation of a functional lac regulatory region.

Authors:  A Landy; E Olchowski; W Ross; G Reiness
Journal:  Mol Gen Genet       Date:  1974

5.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

6.  Structure and function of the genome of coliphage T5. Transcription in vitro of the "nicked" and "nick-free" T5+ DNA.

Authors:  K Knopf; H Bujard
Journal:  Eur J Biochem       Date:  1975-05-06

7.  Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons.

Authors:  F Cabello; K Timmis; S N Cohen
Journal:  Nature       Date:  1976-01-29       Impact factor: 49.962

8.  Organization of transcriptional signals in plasmids pBR322 and pACYC184.

Authors:  D Stüber; H Bujard
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

9.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

10.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688
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  61 in total

1.  Hyper-inducible expression system for streptomycetes.

Authors:  Sachio Herai; Yoshiteru Hashimoto; Hiroki Higashibata; Hideaki Maseda; Haruo Ikeda; Satoshi Omura; Michihiko Kobayashi
Journal:  Proc Natl Acad Sci U S A       Date:  2004-09-17       Impact factor: 11.205

Review 2.  High-expression of a target gene and high-stability of the plasmid.

Authors:  M Kobayashi; Y Kurusu; H Yukawa
Journal:  Appl Biochem Biotechnol       Date:  1991-02       Impact factor: 2.926

3.  The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency.

Authors:  S A Emory; J G Belasco
Journal:  J Bacteriol       Date:  1990-08       Impact factor: 3.490

4.  Cloning in Escherichia coli of a Bacillus subtilis arginine repressor gene through its ability to confer structural stability on a fragment carrying genes of arginine biosynthesis.

Authors:  M C Smith; A Mountain; S Baumberg
Journal:  Mol Gen Genet       Date:  1986-10

5.  Cloning and characterization of promoter-active DNA sequences from Streptococcus equisimilis.

Authors:  Jagdeep Kaur; G Rajamohan; Kanak L Dikshit
Journal:  Curr Microbiol       Date:  2006-12-13       Impact factor: 2.188

6.  Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis.

Authors:  D G Yansura; D J Henner
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

7.  Transcription analysis of the prolate-headed lactococcal bacteriophage c2.

Authors:  M W Lubbers; K Schofield; N R Waterfield; K M Polzin
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

8.  A computer algorithm for testing potential prokaryotic terminators.

Authors:  V Brendel; E N Trifonov
Journal:  Nucleic Acids Res       Date:  1984-05-25       Impact factor: 16.971

9.  The region of phage T4 genes 34, 33 and 59: primary structures and organization on the genome.

Authors:  S Hahn; U Kruse; W Rüger
Journal:  Nucleic Acids Res       Date:  1986-12-09       Impact factor: 16.971

10.  Use of chromosomal integration in the establishment and expression of blaZ, a Staphylococcus aureus beta-lactamase gene, in Bacillus subtilis.

Authors:  C W Saunders; B J Schmidt; M S Mirot; L D Thompson; M S Guyer
Journal:  J Bacteriol       Date:  1984-03       Impact factor: 3.490
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