Uxua Ortecho-Zuta1, Carla Caroline de Oliveira Duque1, Rafael Antonio de Oliveira Ribeiro1, Maria Luísa Leite1, Diana Gabriela Soares2, Josimeri Hebling3, André Luiz Fraga Briso4, Carlos Alberto de Souza Costa5. 1. Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, São Paulo State University (Unesp), Rua Humaitá, 1680, Araraquara, SP, 14801-903, Brazil. 2. Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Rua Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil. 3. Departament of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, University São Paulo State (Unesp), Rua Humaitá, 1680, Araraquara, SP, 14801-903, Brazil. 4. Department of Restorative Dentistry, Araçatuba, School of Dentistry University São Paulo State University (Unesp), Rua José Bonifácio 1193, Vila Mendonça, 16015-050, Brazil. 5. Department of Physiology and Pathology, Araraquara School of Dentistry, São Paulo State University (Unesp), Rua Humaitá, Araraquara, SP, 168014801-903, Brazil. cas.costa@unesp.br.
Abstract
OBJECTIVES: Evaluate in vitro the esthetic efficacy and cytotoxicity of a bleaching gel containing 35% hydrogen peroxide (BG-35%H2O2), applied for different time intervals, on enamel coated or not with polymeric biomaterials. MATERIALS AND METHODS: Nanofiber scaffolds (NSc) and a primer catalyst (PrCa) were used to coat the bovine enamel/dentin discs before the application of BG-35%H2O2, according to the following groups: G1-negative control (NC, without treatment); G2, G3, and G4-BG-35%H2O2 applied for 3 × 15, 2 × 15, and 15 min; G5, G6, and G7-BG-35%H2O2 applied on enamel coated with NSc and PrCa for 3 × 15; 2 × 15, and 15 min, respectively. The culture medium with components of gel diffused through the discs was applied on MDPC-23 cells, which were evaluated regarding to viability (VB), integrity of the membrane (IM), and oxidative stress (OxS). The quantity of H2O2 diffused and esthetic efficacy (ΔE/ΔWI) of the dental tissues were also analyzed (ANOVA/Tukey; p < 0.05). RESULTS: Only G7 was similar to G1 regarding VB (p > 0.05). The lowest value of H2O2 diffusion occurred in G4 and G7, where the cells exhibited the lowest OxS than G2 (p < 0.05). Despite G5 showing the greatest ΔE regarding other groups (p < 0.05), the esthetic efficacy observed in G7 was similar to G2 (p > 0.05). ΔWI indicated a greater bleaching effect for groups G5, G6, and G7 (p < 0.05). CONCLUSION: Coating the dental enamel with polymeric biomaterials reduced the time and the cytotoxicity of BG-35%H2O2. CLINICAL SIGNIFICANCE: Coating the dental enamel with polymeric biomaterials allows safer and faster BG-35%H2O2 application.
OBJECTIVES: Evaluate in vitro the esthetic efficacy and cytotoxicity of a bleaching gel containing 35% hydrogen peroxide (BG-35%H2O2), applied for different time intervals, on enamel coated or not with polymeric biomaterials. MATERIALS AND METHODS: Nanofiber scaffolds (NSc) and a primer catalyst (PrCa) were used to coat the bovine enamel/dentin discs before the application of BG-35%H2O2, according to the following groups: G1-negative control (NC, without treatment); G2, G3, and G4-BG-35%H2O2 applied for 3 × 15, 2 × 15, and 15 min; G5, G6, and G7-BG-35%H2O2 applied on enamel coated with NSc and PrCa for 3 × 15; 2 × 15, and 15 min, respectively. The culture medium with components of gel diffused through the discs was applied on MDPC-23 cells, which were evaluated regarding to viability (VB), integrity of the membrane (IM), and oxidative stress (OxS). The quantity of H2O2 diffused and esthetic efficacy (ΔE/ΔWI) of the dental tissues were also analyzed (ANOVA/Tukey; p < 0.05). RESULTS: Only G7 was similar to G1 regarding VB (p > 0.05). The lowest value of H2O2 diffusion occurred in G4 and G7, where the cells exhibited the lowest OxS than G2 (p < 0.05). Despite G5 showing the greatest ΔE regarding other groups (p < 0.05), the esthetic efficacy observed in G7 was similar to G2 (p > 0.05). ΔWI indicated a greater bleaching effect for groups G5, G6, and G7 (p < 0.05). CONCLUSION: Coating the dental enamel with polymeric biomaterials reduced the time and the cytotoxicity of BG-35%H2O2. CLINICAL SIGNIFICANCE: Coating the dental enamel with polymeric biomaterials allows safer and faster BG-35%H2O2 application.
Authors: Ieb Martins; S Onofre; N Franco; L M Martins; A Montenegro; L A Arana-Gordillo; A Reis; A D Loguercio; L M da Silva Journal: Oper Dent Date: 2018 Jul/Aug Impact factor: 2.440
Authors: Douglas Augusto Roderjan; Rodrigo Stanislawczuk; Josimeri Hebling; Carlos Alberto de Souza Costa; Alessandra Reis; Alessandro D Loguercio Journal: Braz Dent J Date: 2015 May-Jun
Authors: L M Martins; L A Lima E Souza; E Sutil; L M da Silva; Jos Silva; A Reis; A D Loguercio Journal: Oper Dent Date: 2020 Jan/Feb Impact factor: 2.440