Sirt3, 6, and 7 Genes Expression in Gastric Antral Epithelial Cells of Patients with Helicobacter pylori Infection.

Sirtuins, known as the intracellular acylation enzymes, play a major role in regulating the cell's physiological activities. The relevant studies have shown diversely sirtuin genes expression in various cancers in humans. This study has surveyed the transcription of sirt3, 6, and 7 genes in gastric antral epithelial cells (GAECs) of gastritis and gastric adenocarcinoma patients with and without Helicobacter pylori infection. First of all, a case-control study was conducted, including 50 and 53 gastric antral biopsy samples collected from gastritis and gastric adenocarcinoma patients with and without H. pylori infection referred to hospitals of Sanandaj City during 2018-2019. Total RNAs were extracted from biopsy samples, then cDNAs were synthesized by using TaKaRa kits. Quality essay of H. pylori virulence genes expression and relative quantitative essay of sirt3, 6, and 7 genes expressions in gastric antral biopsy samples were performed using the real-time RT-PCR method. The statistical analysis showed the significant correlations between H. pylori vacA s1m2 and sabA cDNAs with sirt3 geneś expression in GAECs (P < 0.05, 0.05 respectively). In addition, sirt6 gene's expression decreased along increasing age in gastric adenocarcinoma patients (P < 0.05). The samples of gastritis patients with gastric antral epithelial biopsy containing H. pylori hopQII, oipA, and sabB cDNA showed an increased amount of sirt7 genes expression (P < 0.05, 0.05, and 0.05 respectively). In conclusion, the H. pylori virulence genes expression and increasing age of patients showed the significant correlations with sirt3, 6, and 7 genes expressions in GAECs of gastric and gastric cancer patients.