| Literature DB >> 35180378 |
Jue Feng1, Joseph N Pucella2, Geunhyo Jang2, Marcela Alcántara-Hernández3, Samik Upadhaya2, Nicholas M Adams2, Alireza Khodadadi-Jamayran4, Colleen M Lau2, Marlon Stoeckius5, Stephanie Hao5, Peter Smibert5, Aristotelis Tsirigos4, Juliana Idoyaga3, Boris Reizis6.
Abstract
Developmental origins of dendritic cells (DCs) including conventional DCs (cDCs, comprising cDC1 and cDC2 subsets) and plasmacytoid DCs (pDCs) remain unclear. We studied DC development in unmanipulated adult mice using inducible lineage tracing combined with clonal DNA "barcoding" and single-cell transcriptome and phenotype analysis (CITE-seq). Inducible tracing of Cx3cr1+ hematopoietic progenitors in the bone marrow showed that they simultaneously produce all DC subsets including pDCs, cDC1s, and cDC2s. Clonal tracing of hematopoietic stem cells (HSCs) and of Cx3cr1+ progenitors revealed clone sharing between cDC1s and pDCs, but not between the two cDC subsets or between pDCs and B cells. Accordingly, CITE-seq analyses of differentiating HSCs and Cx3cr1+ progenitors identified progressive stages of pDC development including Cx3cr1+ Ly-6D+ pro-pDCs that were distinct from lymphoid progenitors. These results reveal the shared origin of pDCs and cDCs and suggest a revised scheme of DC development whereby pDCs share clonal relationship with cDC1s.Entities:
Keywords: conventional dendritic cells; hematopoietic progenitors; hematopoietic stem cells; lineage tracing; plasmacytoid dendritic cells
Mesh：
Year: 2022 PMID： 35180378 PMCID： PMC9344860 DOI： 10.1016/j.immuni.2022.01.016
Source DB: PubMed Journal: Immunity ISSN： 1074-7613 Impact factor: 43.474