Literature DB >> 3517861

Human seminal alpha-inhibins: detection in human pituitary, hypothalamus, and serum by immunoreactivity.

K Ramasharma, C H Li.   

Abstract

An antiserum generated in rabbits against human seminal alpha-inhibin-52 has been used to develop a sensitive radioimmunoassay for the detection of alpha-inhibins. The alpha-inhibin-52 antiserum reacts with alpha-inhibin-92 and alpha-inhibin-31 with equal avidity. These peptides were found to be present in human pituitary, hypothalamus, and serum. In exclusion chromatography on Sephadex G-100, the immunoreactive material eluted in a large molecular size region. Immunoblot analysis of column-derived fractions of these extracts revealed the presence of alpha-inhibin-92. The mean concentrations of immunoreactive alpha-inhibin were found to be 7.2 ng/ml in normal adult male serum, 70.3 ng/g (wet weight) of pituitary, and 12.9 ng/g (wet weight) of hypothalamus. This communication reports on the evidence for the existence of gonadal peptides in the brain.

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Year:  1986        PMID： 3517861      PMCID： PMC323540          DOI： 10.1073/pnas.83.10.3484

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  16 in total

Review 1.  Inhibin: from concept to reality.

Authors:  P Franchimont; J Verstraelen-Proyard; M T Hazee-Hagelstein; C Renard; A Demoulin; J P Bourguignon; J Hustin
Journal:  Vitam Horm       Date:  1979       Impact factor: 3.421

2.  Isolation and partial characterization of a Mr 32,000 protein with inhibin activity from porcine follicular fluid.

Authors:  N Ling; S Y Ying; N Ueno; F Esch; L Denoroy; R Guillemin
Journal:  Proc Natl Acad Sci U S A       Date:  1985-11       Impact factor: 11.205

Review 3.  The evidence that inhibin must exist.

Authors:  S J Main; R V Davies; B P Setchell
Journal:  J Reprod Fertil Suppl       Date:  1979

4.  Complete amino acid sequence of human seminal plasma beta-inhibin. Prediction of post Gln-Arg cleavage as a maturation site.

Authors:  N G Seidah; N J Arbatti; J Rochemont; A R Sheth; M Chrétien
Journal:  FEBS Lett       Date:  1984-10-01       Impact factor: 4.124

5.  Isolation and characterization of inhibin from human seminal plasma.

Authors:  K Ramasharma; M R Sairam
Journal:  Ann N Y Acad Sci       Date:  1982       Impact factor: 5.691

Review 6.  Physiology and biochemistry of ovarian inhibin.

Authors:  C P Channing; W L Gordon; W K Liu; D N Ward
Journal:  Proc Soc Exp Biol Med       Date:  1985-03

7.  Isolation, structure, and synthesis of a human seminal plasma peptide with inhibin-like activity.

Authors:  K Ramasharma; M R Sairam; N G Seidah; M Chrétien; P Manjunath; P W Schiller; D Yamashiro; C H Li
Journal:  Science       Date:  1984-03-16       Impact factor: 47.728

8.  Effect of inhibin like factors on gonadotrophin release by the mouse pituitary in vitro.

Authors:  K Ramasharma; M R Sairam; M R Ranganathan
Journal:  Acta Endocrinol (Copenh)       Date:  1981-12

9.  Human seminal alpha inhibins: isolation, characterization, and structure.

Authors:  C H Li; R G Hammonds; K Ramasharma; D Chung
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

10.  Purification and partial characterization of inhibin from porcine follicular fluid.

Authors:  J Rivier; J Spiess; R McClintock; J Vaughan; W Vale
Journal:  Biochem Biophys Res Commun       Date:  1985-11-27       Impact factor: 3.575
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  3 in total

1.  Characteristics of binding of human seminal alpha-inhibin-92 to human pituitary membranes.

Authors:  K Ramasharma; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

2.  Sex steroid hormone regulation of follicle-stimulating hormone subunit messenger ribonucleic acid (mRNA) levels in the rat.

Authors:  S D Gharib; M E Wierman; T M Badger; W W Chin
Journal:  J Clin Invest       Date:  1987-08       Impact factor: 14.808

3.  Inhibin-like and gonadotropin-like immunoreactivity in pituitary cells of male monkeys (Macaca fascicularis, Macaca mulatta).

Authors:  S Schlatt; G F Weinbauer; E Nieschlag
Journal:  Cell Tissue Res       Date:  1991-08       Impact factor: 5.249
  3 in total

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