Dejun Wang1, Xue Song1, Nan Zhang1, Yesong Guo1. 1. Department of Radiotherapy, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University Nanjing, Jiangsu Province, China.
Abstract
OBJECTIVE: To investigate the function and mechanism of miR-1179 in the tumorigenesis of hepatocellular carcinoma (HCC). METHODS: The levels of miR-1179 and NUAK2 in clinical tissues or cell lines were examined using quantitative Real-time PCR (qRT-PCR). Cell Counting Kit-8 (CCK-8), EdU assay, colony formation, wound healing, Transwell assays and flow cytometry assays were conducted to examine the impact of miR-1179 on HCC cells. The protein expression of NUAK2 was detected using Western blotting assay. Bioinformatics analysis and luciferase reporter assays were conducted to reveal the association of miR-1179 with NUAK2. RESULTS: MiR-1179 expression was significantly downregulated in HCC specimens and cell lines compared to normal samples and cells. The miR-1179 overexpression inhibited HCC cell migration, invasion and proliferation through targeting NUAK2. Overexpression of NUAK2 can reverse the effect of miR-1179 on hepatocellular carcinoma cells. CONCLUSION: miR-1179 suppresses HCC developmen through targeting NUAK2. Which can be used as a potent HCC diagnostic marker. AJTR
OBJECTIVE: To investigate the function and mechanism of miR-1179 in the tumorigenesis of hepatocellular carcinoma (HCC). METHODS: The levels of miR-1179 and NUAK2 in clinical tissues or cell lines were examined using quantitative Real-time PCR (qRT-PCR). Cell Counting Kit-8 (CCK-8), EdU assay, colony formation, wound healing, Transwell assays and flow cytometry assays were conducted to examine the impact of miR-1179 on HCC cells. The protein expression of NUAK2 was detected using Western blotting assay. Bioinformatics analysis and luciferase reporter assays were conducted to reveal the association of miR-1179 with NUAK2. RESULTS: MiR-1179 expression was significantly downregulated in HCC specimens and cell lines compared to normal samples and cells. The miR-1179 overexpression inhibited HCC cell migration, invasion and proliferation through targeting NUAK2. Overexpression of NUAK2 can reverse the effect of miR-1179 on hepatocellular carcinoma cells. CONCLUSION: miR-1179 suppresses HCC developmen through targeting NUAK2. Which can be used as a potent HCC diagnostic marker. AJTR
Authors: Andreas Karakatsanis; Ioannis Papaconstantinou; Maria Gazouli; Anna Lyberopoulou; George Polymeneas; Dionysios Voros Journal: Mol Carcinog Date: 2011-12-27 Impact factor: 4.784
Authors: Christoph Herold; Thomas Reck; Peter Fischler; Rudolf Ott; Martin Radespiel-Troeger; Marion Ganslmayer; Werner Hohenberger; Eckhart G Hahn; Detlef Schuppan Journal: Liver Date: 2002-02