Literature DB >> 3514

Chromatography of hemoglobins on CM-cellulose with bis-tris and sodium chloride developers.

W A Schroeder, L A Pace.   

Abstract

CM-Cellulose as an ion-exchange medium with Bis-tris as buffer and a gradient of sodium chloride provides a versatile system for the chromatography of hemoglobins. Changes in pH, Bis-tris concentration, and slope of the sodium chloride grandient provide means for markedly altering chromatographic behavior for special separations. Examples are given of the application of the method to normal samples and to those with hemoglobinopathies.

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Year:  1976        PMID: 3514     DOI: 10.1016/s0021-9673(00)82166-4

Source DB:  PubMed          Journal:  J Chromatogr


  5 in total

1.  A monoclonal antibody-linked immunoassay for hemoglobin H disease.

Authors:  M Shyamala; C R Kiefer; H Moscoso; F A Garver
Journal:  Ann Hematol       Date:  1992-07       Impact factor: 3.673

2.  Identification of eleven human hemoglobin variants by high-performance liquid chromatography: additional data on functional properties and clinical expression.

Authors:  W A Schroeder; J B Shelton; J R Shelton; D Powars; S Friedman; J Baker; J Z Finklestein; B Miller; C S Johnson; J R Sharpsteen; L Sieger; E Kawaoka
Journal:  Biochem Genet       Date:  1982-02       Impact factor: 1.890

3.  Modification of hemoglobin H disease by sickle trait.

Authors:  K K Matthay; W C Mentzer; A M Dozy; Y W Kan; D F Bainton
Journal:  J Clin Invest       Date:  1979-10       Impact factor: 14.808

4.  Biosynthesis of cat hemoglobins: translation of poly(A)-RNA from animals of various HbA/HbB phenotypes.

Authors:  J Kasten-Jolly; F Taketa
Journal:  Biochem Genet       Date:  1984-10       Impact factor: 1.890

5.  Prenatal diagnosis of hemoglobinopathies: evaluation of techniques for analysing globin-chain synthesis in blood samples obtained by fetoscopy.

Authors:  L F Congote; E F Hamilton; J C Chow; T B Perry
Journal:  Can Med Assoc J       Date:  1982-11-01       Impact factor: 8.262

  5 in total

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