Literature DB >> 3512520

Properties of an ordered ring structure formed by recombinant Treponema pallidum surface antigen 4D.

T E Fehniger, J D Radolf, M A Lovett.   

Abstract

Ultrastructural and biochemical studies of a recombinant Treponema pallidum surface antigen designated 4D have been conducted due to its likely biological significance. Electron microscopy demonstrated that the 190-kilodalton (kDa) 4D molecule is an ordered ring structure of 10-nm diameter. The 90-kDa proteinase K-treated 4D is an ordered ring structure of 6-nm diameter. Evidence is presented that the 190-kDa ordered ring is maintained by noncovalent bonds; 19-kDa monomers can reassociate in vitro to reform a 190-kDa molecule. Amino acid composition analysis of 190-kDa 4D showed that the molecule is composed of 45% hydrophobic residues. Evidence relating the structure of the 4D ordered ring to its potential role in the pathogenesis of syphilis is discussed.

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Year:  1986        PMID： 3512520      PMCID： PMC214490          DOI： 10.1128/jb.165.3.732-739.1986

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  Primary structure of major outer membrane protein I of Escherichia coli B/r.

Authors:  R Chen; C Krämer; W Schmidmayr; U Henning
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

2.  Subunit structure of functional porin oligomers that form permeability channels in the other membrane of Escherichia coli.

Authors:  T Nakae; J Ishii; M Tokunaga
Journal:  J Biol Chem       Date:  1979-03-10       Impact factor: 5.157

3.  Role of disulfide bonding in outer membrane structure and permeability in Chlamydia trachomatis.

Authors:  P Bavoil; A Ohlin; J Schachter
Journal:  Infect Immun       Date:  1984-05       Impact factor: 3.441

Review 4.  Molecular architecture and functioning of the outer membrane of Escherichia coli and other gram-negative bacteria.

Authors:  B Lugtenberg; L Van Alphen
Journal:  Biochim Biophys Acta       Date:  1983-03-21

5.  Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12.

Authors:  R Chen; W Schmidmayr; C Krämer; U Chen-Schmeisser; U Henning
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

6.  Abrogation of genetically controlled resistance of mice to Treponema pallidum by irradiation.

Authors:  J R Klein; A A Monjan; P H Hardy; G A Cole
Journal:  Nature       Date:  1980-02-07       Impact factor: 49.962

7.  Surface-associated host proteins on virulent Treponema pallidum.

Authors:  J F Alderete; J B Baseman
Journal:  Infect Immun       Date:  1979-12       Impact factor: 3.441

8.  Dissociation and reassembly of Escherichia coli type 1 pili.

Authors:  Y Eshdat; F J Silverblatt; N Sharon
Journal:  J Bacteriol       Date:  1981-10       Impact factor: 3.490

9.  Electron microscopy of treponemes subjected to the Treponema pallidum immobilization (TPI) test. II. Immunoelectron microscopy.

Authors:  K Hovind-Hougen; A Birch-Andersen; H A Nielsen
Journal:  Acta Pathol Microbiol Scand C       Date:  1979-08

10.  Surface characterization of virulent Treponema pallidum.

Authors:  J F Alderete; J B Baseman
Journal:  Infect Immun       Date:  1980-12       Impact factor: 3.441
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  14 in total

1.  Dps protects cells against multiple stresses during stationary phase.

Authors:  Sudha Nair; Steven E Finkel
Journal:  J Bacteriol       Date:  2004-07       Impact factor: 3.490

2.  Primary structure of an oligomeric antigen of Treponema pallidum.

Authors:  A M Walfield; E S Roche; M C Zounes; H Kirkpatrick; M A Wild; G Textor; P K Tsai; C Richardson
Journal:  Infect Immun       Date:  1989-02       Impact factor: 3.441

3.  A new attempt to distinguish serologically the subspecies of Treponema pallidum causing syphilis and yaws.

Authors:  G T Noordhoek; A Cockayne; L M Schouls; R H Meloen; E Stolz; J D van Embden
Journal:  J Clin Microbiol       Date:  1990-07       Impact factor: 5.948

4.  Selective release of the Treponema pallidum outer membrane and associated polypeptides with Triton X-114.

Authors:  T M Cunningham; E M Walker; J N Miller; M A Lovett
Journal:  J Bacteriol       Date:  1988-12       Impact factor: 3.490

5.  Polymerase chain reaction and synthetic DNA probes: a means of distinguishing the causative agents of syphilis and yaws?

Authors:  G T Noordhoek; B Wieles; J J van der Sluis; J D van Embden
Journal:  Infect Immun       Date:  1990-06       Impact factor: 3.441

6.  Role of disulfide bonds in the oligomeric structure and protease resistance of recombinant and native Treponema pallidum surface antigen 4D.

Authors:  J D Radolf; L A Borenstein; J Y Kim; T E Fehniger; M A Lovett
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

Review 7.  Vaccines for bacterial sexually transmitted infections: a realistic goal?

Authors:  P F Sparling; C Elkins; P B Wyrick; M S Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1994-03-29       Impact factor: 11.205

8.  Native surface association of a recombinant 38-kilodalton Treponema pallidum antigen isolated from the Escherichia coli outer membrane.

Authors:  T E Fehniger; J D Radolf; A M Walfield; T M Cunningham; J N Miller; M A Lovett
Journal:  Infect Immun       Date:  1986-05       Impact factor: 3.441

9.  Lipid modification of the 17-kilodalton membrane immunogen of Treponema pallidum determines macrophage activation as well as amphiphilicity.

Authors:  D R Akins; B K Purcell; M M Mitra; M V Norgard; J D Radolf
Journal:  Infect Immun       Date:  1993-04       Impact factor: 3.441

10.  Characterization of a Helicobacter pylori neutrophil-activating protein.

Authors:  D J Evans; D G Evans; T Takemura; H Nakano; H C Lampert; D Y Graham; D N Granger; P R Kvietys
Journal:  Infect Immun       Date:  1995-06       Impact factor: 3.441
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