Literature DB >> 3516880

Native surface association of a recombinant 38-kilodalton Treponema pallidum antigen isolated from the Escherichia coli outer membrane.

T E Fehniger, J D Radolf, A M Walfield, T M Cunningham, J N Miller, M A Lovett.   

Abstract

A recombinant plasmid designated pAW305, containing a 6-kilobase insert of Treponema pallidum DNA, directed the expression of a 38-kilodalton (kDa) treponemal antigen in Escherichia coli. The 38-kDa antigen copurified with the outer membrane fraction of the E. coli cell envelope after treatment with nonionic detergents or sucrose density gradient centrifugation. Rabbits immunized with the recombinant 38-kDa antigen developed antibodies which reacted specifically with a 38-kDa T. pallidum antigen on immunoblots, and 38-kDa antisera specifically immobilized T. pallidum in a complement-dependent manner in the T. pallidum immobilization test. Antisera to the 38-kDa recombinant antigen were also used to demonstrate its native surface association on T. pallidum by immunoelectron microscopy.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3516880      PMCID： PMC261041          DOI： 10.1128/iai.52.2.586-593.1986

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  29 in total

1.  INFLUENCE OF LYSOZYME UPON THE REACTIVITY OF TREPONEMA PALLIDUM IN THE FLUORESCENT ANTIBODY REACTION.

Authors:  M METZGER; J RUCZKOWSKA
Journal:  Arch Immunol Ther Exp (Warsz)       Date:  1964       Impact factor: 4.291

2.  Primary structure of major outer membrane protein I of Escherichia coli B/r.

Authors:  R Chen; C Krämer; W Schmidmayr; U Henning
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

3.  Fluorescent treponemal antibody-absorption (FTA-ABS) test for syphilis.

Authors:  W E Deacon; J B Lucas; E V Price
Journal:  JAMA       Date:  1966-11-07       Impact factor: 56.272

4.  Peptidoglycan-associated outer membrane proteins in gammegatine bacteria.

Authors:  B Lugtenberg; H Bronstein; N van Selm; R Peters
Journal:  Biochim Biophys Acta       Date:  1977-03-17

5.  Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12.

Authors:  R Chen; W Schmidmayr; C Krämer; U Chen-Schmeisser; U Henning
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

6.  Surface-associated host proteins on virulent Treponema pallidum.

Authors:  J F Alderete; J B Baseman
Journal:  Infect Immun       Date:  1979-12       Impact factor: 3.441

7.  Humoral immunity in experimental syphilis. II. The relationship of neutralizing factors in immune serum to acquired resistance.

Authors:  N H Bishop; J N Miller
Journal:  J Immunol       Date:  1976-07       Impact factor: 5.422

8.  Surface mucopolysaccharides of Treponema pallidum.

Authors:  T J Fitzgerald; R C Johnson
Journal:  Infect Immun       Date:  1979-04       Impact factor: 3.441

9.  Electron microscopy of treponemes subjected to the Treponema pallidum immobilization (TPI) test. II. Immunoelectron microscopy.

Authors:  K Hovind-Hougen; A Birch-Andersen; H A Nielsen
Journal:  Acta Pathol Microbiol Scand C       Date:  1979-08

10.  Molecular characterization of receptor binding proteins and immunogens of virulent Treponema pallidum.

Authors:  J B Baseman; E C Hayes
Journal:  J Exp Med       Date:  1980-03-01       Impact factor: 14.307
View more
  20 in total

Review 1.  Biological basis for syphilis.

Authors:  Rebecca E Lafond; Sheila A Lukehart
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

2.  Expression in Escherichia coli of the 37-kilodalton endoflagellar sheath protein of Treponema pallidum by use of the polymerase chain reaction and a T7 expression system.

Authors:  R D Isaacs; J D Radolf
Journal:  Infect Immun       Date:  1990-07       Impact factor: 3.441

3.  Demonstration of rare protein in the outer membrane of Treponema pallidum subsp. pallidum by freeze-fracture analysis.

Authors:  E M Walker; G A Zampighi; D R Blanco; J N Miller; M A Lovett
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490

4.  Identification of Treponema pallidum penicillin-binding proteins.

Authors:  T M Cunningham; J N Miller; M A Lovett
Journal:  J Bacteriol       Date:  1987-11       Impact factor: 3.490

5.  Selective release of the Treponema pallidum outer membrane and associated polypeptides with Triton X-114.

Authors:  T M Cunningham; E M Walker; J N Miller; M A Lovett
Journal:  J Bacteriol       Date:  1988-12       Impact factor: 3.490

6.  The outer membrane, not a coat of host proteins, limits antigenicity of virulent Treponema pallidum.

Authors:  D L Cox; P Chang; A W McDowall; J D Radolf
Journal:  Infect Immun       Date:  1992-03       Impact factor: 3.441

7.  Changes in the cell surface properties of Treponema pallidum that occur during in vitro incubation of freshly extracted organisms.

Authors:  L V Stamm; R L Hodinka; P B Wyrick; P J Bassford
Journal:  Infect Immun       Date:  1987-09       Impact factor: 3.441

8.  Identification and localization of integral membrane proteins of virulent Treponema pallidum subsp. pallidum by phase partitioning with the nonionic detergent triton X-114.

Authors:  J D Radolf; N R Chamberlain; A Clausell; M V Norgard
Journal:  Infect Immun       Date:  1988-02       Impact factor: 3.441

9.  Isolation of the outer membranes from Treponema pallidum and Treponema vincentii.

Authors:  D R Blanco; K Reimann; J Skare; C I Champion; D Foley; M M Exner; R E Hancock; J N Miller; M A Lovett
Journal:  J Bacteriol       Date:  1994-10       Impact factor: 3.490

10.  Porin activity and sequence analysis of a 31-kilodalton Treponema pallidum subsp. pallidum rare outer membrane protein (Tromp1).

Authors:  D R Blanco; C I Champion; M M Exner; H Erdjument-Bromage; R E Hancock; P Tempst; J N Miller; M A Lovett
Journal:  J Bacteriol       Date:  1995-06       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.