Part I Antimicrobial resistance: Bacterial pathogens of dermatologic significance and implications of rising resistance.

Although the COVID-19 pandemic has been the defining global health crisis of our time, public health officials have been sounding the alarm of another ominous threat for years: an impending antimicrobial resistance crisis. In dermatology, antibiotics are often used for prolonged courses in the treatment of skin and soft tissue infections and common inflammatory skin conditions, increasing the risk of microbiome alteration and antibiotic-related adverse effects, all while exerting consequential selective pressures on both pathogenic and bystander bacteria. In this review, we hope to raise awareness of the crisis of antimicrobial resistance and review resistance concerns related to dermatology-relevant bacterial pathogens.

Learning objectives

After completing this learning objective, the reader will be able to better discuss the causes and clinical implications of antimicrobial resistance among bacterial pathogens of dermatologic significance.

Disclosures

None.

Editors

The editors involved with this CME activity and all content validation/peer reviewers of the journal-based CME activity have reported no relevant financial relationships with commercial interest(s).

Authors

The authors involved with this journal-based CME activity have reported no relevant financial relationships with commercial interest(s).

Planners

The planners involved with this journal-based CME activity have reported no relevant financial relationships with commercial interest(s). The editorial and education staff involved with this journal-based CME activity have reported no relevant financial relationships with commercial interest(s).

Introduction

One of the greatest global public health threats of the 21st century is growing antimicrobial resistance (AMR). Concerns for a looming AMR crisis may have been temporarily overshadowed by the COVID-19 pandemic, but many experts worry that pandemic-induced increases in antibiotic exposures, hospitalizations, and diverted resources have only accelerated its arrival.1, 2, 3, 4

Since penicillin’s discovery in 1928, antibiotic therapy has been a foundational pillar of modern medicine. Unfortunately, the rate at which bacterial pathogens are becoming resistant to currently available antibiotics has consistently outpaced the rate of new antibiotic development. The Centers for Disease Control and Prevention estimate that AMR infections already cause 1 death every 15 minutes in the United States. Globally, AMR has become a top public health concern , and is now responsible for more deaths than HIV/AIDS or malaria; In 2019 alone, drug resistant bacterial infections were associated with an estimated 4.95 million deaths worldwide, including 1.27 million deaths that were directly attributable to antibiotic resistance. Resistance among Staphylococcus aureus, Streptococcus. pyogenes, and Pseudomonas aeruginosa are among those that the Centers for Disease Control and World Health Organization consider prioritized threats. ,

The development of an AMR crisis, fueled largely by the overuse and misuse of antibiotics, should be a rallying call for increased antibiotic stewardship.9, 10, 11 The Centers for Disease Control and Prevention estimate that 50% of outpatient antibiotics are inappropriately prescribed based on agent selection, dosing, or duration and at least 30% of outpatient antibiotics are given unnecessarily. , Since over 80% of all human antibiotic use occurs in the outpatient setting, outpatient prescribing patterns are a critical target for stewardship efforts. The overuse of broad-spectrum antibiotics is particularly concerning in terms of their potential to induce wider microbiome disturbances and resistance among bacteria. Broad-spectrum antibiotics such as mupirocin, doxycycline, minocycline, and trimethoprim-sulfamethoxazole are valuable therapeutic agents in dermatology, but the consequences of their widespread use for narrow-spectrum indications must be considered. In this article, we review key dermatology-relevant issues related to bacterial AMR.

Discussion

Methicillin-resistant Staphylococcus aureus

Key points

Methicillin resistance in S aureus (MRSA) occurs due to alterations in penicillin-binding proteins (PBPs) and can be transferred on a mobile genetic element.

Hospital-acquired, community-acquired, and livestock-associated strains of MRSA are distinct strains, but their clinical and epidemiologic distinctions are blurring.

S aureus colonizes 20% to 40% of the population and causes infections of almost every body site, with skin involvement being the most common (Table I ).15, 16, 17 S aureus exemplifies the remarkable ability of bacteria to adapt to selective antibiotic pressures. Within just 2 years of penicillin’s introduction, penicillinase - producing - resistant S aureus clones emerged, and by the 1970s, 95% of S aureus isolates were penicillin resistant.16, 17, 18, 19 Efforts to synthesize agents impervious to penicillinase led to the discovery of methicillin in 1959, to which S aureus responded with resistance within a year.

Table I

Methicillin-resistant Staphylococcus aureus antibiotic options and resistance mechanisms17, 18, 19, 20, 21, 22, 23

Agent	Mechanism of action	Resistance mechanism
TMP-SMX	Sequential inhibition of nucleic acid synthesis: SMX inhibits synthesis of dihydrofolic acid by dihydropteroate synthetase (DHPS) and TMP inhibits synthesis of tetrahydrofolate by dihydrofolate reductase (DHFR)	Altered drug target and decreased drug binding via point mutations in DHFR and DHPS
DoxycyclineMinocycline	Binding to 16S rRNA within 30S ribosomal subunit → inhibition of protein synthesis	1) Active drug efflux via tetracycline efflux pumps encoded by plasmid-borne genes Tet (K) and Tet (L)2) Ribosomal target protection via transposon-encoded genes tet (M) and tet (O) that catalyze tetracycline dissociation from the ribosome
Clindamycin	Binding to 23S rRNA of 50S ribosomal subunit → inhibition of protein synthesis	Modification of drug-binding site via erm gene, an enzyme that methylates the 23S rRNA → variable cross-resistance to macrolides, lincosamides, and streptogramin B (MLSb phenotype)
Vancomycin	Binding to D-ala-D-ala residues → inhibition of peptidoglycan cell-wall synthesis	VISA: inhibition of drug binding to cell-wall target via mutations in genes regulating cell-wall synthesisVRSA: Reduced drug binding to target via acquisition of vanA, a plasmid element from Enterococcus spp that encodes a modified D-ala-D-lac precursor
Teicoplanin	Binding to D-ala-D-ala residues → inhibition of peptidoglycan cell-wall synthesis	Decreased drug affinity to target via modified peptidoglycan precursors
Fluoroquinolones	Inhibition of enzymes involved in DNA replication, DNA gyrase (topoisomerase II) and topoisomerase IV	1) Alteration of target site via mutations in genes encoding topoisomerase II (gyrA and gyrB) and topoisomerase IV (grlA and grlB)2) Active drug efflux via overexpression of efflux pumps NorA and NorB
Linezolid	Binding to of the 23S rRNA of 50S ribosomal subunit → protein synthesis inhibition	1) Decreased drug binding via point mutations in the ribosomal binding site2) Modification of the ribosomal binding site via enzymatic methylation mediated by cfr gene
Daptomycin	Binding and inserting into the bacterial cytoplasmic membrane → damage to the cell membrane and to the membrane potential	1) Modification in the peptidoglycan layer via mpfF, an enzyme that incorporates a lysine residue → inhibits binding of daptomycin2) Changes in phospholipid metabolism via genes encoding cardiolipin synthetases (pgsA, cls) → decreased drug binding
Aminoglycosides	Binding to 16S rRNA of the 30S ribosomal subunit → inhibition of protein synthesis	Enzymatic modification of the drug → decreased drug binding to its target
Fifth generation cephalosporins (Ceftaroline, Ceftobiprole)	Beta-lactams with high affinity for PBP2a/PBP2c proteins	Resistance to ceftaroline has been reported but the mechanisms are unclear
Quinupristin/Dalfopristin	Sequential binding to 50S ribosomal subunit → inhibition of protein synthesis	1) Modification of ribosomal subunit via acquisition of erm genes encoding methylases → decreased drug binding2) Enzymatic degradation of the drug via acquisition of acetyltransferases

MLSb, Macrolide-lincosamide-streptogramin B; MRSA, methicillin-resistant Staphylococcus aureus; PBP, penicillin-binding protein; TMP-SMX, trimethoprim-sulfamethoxazole; VISA, vancomycin-intermediate Staphylococcus aureus; VRSA, vancomycin-resistant Staphylococcus aureus.

Methicillin resistance arises via the alteration of PBPs. Methicillin and other ß-lactam antibiotics bind to PBPs to block key cross-linking steps in bacterial cell-wall synthesis. MRSA isolates express low-affinity PBPs, PBP2a and PBP2c, that are encoded by the mecA and mecC genes, respectively, and carried on a mobile genetic element known as the staphylococcal chromosomal cassette. , , Not only can the staphylococcal chromosomal cassette be readily transferred among staphylococcal species, but it can also carry other resistance genes, partially accounting for the multidrug-resistant (MDR) phenotype of MRSA.

In the 1960s and 1970s, MRSA infections occurred primarily as isolated hospital outbreaks among patients with comorbidities. By the 1980s, hospital-acquired MRSA (HA-MRSA) clones had spread worldwide. Current hospital isolates demonstrate resistance rates of over 50% in the United States and exceed 70% in select East Asian countries. , HA-MRSA is now a leading cause of nosocomial bacteremia, pneumonia, and vascular-access- and catheter-associated infections. HA-MRSA strains are typically resistant to fluoroquinolones, macrolides, trimethoprim, and tetracyclines, necessitating the increased use of agents such as vancomycin, daptomycin, and linezolid. , , , Unfortunately, resistance to even these newer agents has already been documented (Table I).

In the 1990s, reports of community outbreaks of skin and soft tissue infections (SSTIs) due to MRSA among otherwise healthy indivuduals with no hospital exposure began appearing. These community- acquired (CA-MRSA) strains were subsequently found to be genetically and phenotypically distinct strains from HA-MRSA and to express virulence toxins such as Panton-Valentine leucocidin that enabled them to infect otherwise healthy hosts. , Whereas HA-MRSA is most often associated with nosocomial infections like bacteremia and endocarditis, over 90% of CA-MRSA infections are purulent SSTIs. Unlike HA-MRSA, CA-MRSA is also typically sensitive to several non-ß-lactam drugs, including clindamycin, trimethoprim-sulfamethoxazole, and tetracyclines. , Despite these differences, distinguishing CA-MRSA and HA-MRSA on epidemiologic grounds has become increasingly difficult. Since the early 2000s, CA-MRSA strains have been implicated in a significant proportion of hospital-acquired infections, including bacteremia, postsurgical infections, and necrotizing infections in newborns. , Growing antibiotic exposure has also led to the development of MDR CA-MRSA, thereby blurring the susceptibility differences between CA-MRSA and HA-MRSA isolates. , ,

Another new MRSA lineage, livestock-associated MRSA (LA-MRSA), was first reported in pigs in 2005 and has since been documented among other domesticated animals, veterinarians, farmers, and patients from rural settings. , Due to the routine antibiotics used in livestock farming, LA-MRSA is widespread among livestock and readily capable of spreading to humans. Livestock workers are at a significantly higher risk for LA-MRSA colonization and subsequent infection than the general population. Currently, LA-MRSA accounts for 15% of community-acquired SSTIs and 1% to 2% of nosocomial infections; multidrug resistance is also observed in LA-MRSA. As with other S aureus strains, carriers are at risk of subsequent infection and serve as important reservoirs for person-to-person transmission. The emergence of LA-MRSA highlights concerns about the development of AMR with widespread antibiotic use in animal husbandry and the zoonotic transmission of infection and resistance.

The successful dissemination of MRSA poses significant challenges for the management of SSTIs (Fig 1 ). Although CA-MRSA are often sensitive to tetracyclines, trimethoprim-sulfamethoxazole, and clindamycin, resistance to these agents has been reported, and antibiotic selection should be guided by susceptibility testing. Clindamycin’s oral formulation and activity against both S aureus and S pyogenes make it especially useful for the empiric outpatient treatment of SSTIs. Clindamycin resistance occurs with the acquisition of erythromycin-ribosomal-methylase, erm, a gene that encodes for the methylation of the bacterial 23S ribosomal subunit, the shared action site of both macrolides and clindamycin. The resulting cross-resistant phenotype, known as macrolide-lincosamide-streptogramin B (MLSB), is seen in MRSA, methicillin-susceptible S aureus, and S pyogenes. Expression of the erm gene can be either constitutive or induced by macrolide exposure. As would be expected, isolates with constitutive erm expression demonstrate both clindamycin and erythromycin resistance by standard in vitro bacterial resistance testing. Bacteria with macrolide-inducible erm expression test as erythromycin resistant but clindamycin sensitive. Clinically, however, treatment failure with clindamycin occurs as treatment pressure in vivo rapidly selects for mutants that constitutively express erm and are clindamycin resistant. Since clindamycin resistance also occurs via non-erm mediated mechanisms, a specialized susceptibility test, known as the double-disk diffusion, can help identify inducible erm-mediated resistance.

Fig 1

Outpatient management of skin and soft tissue infections from the Centers for Disease Control and Prevention.

I&D, Incision and drainage; FDA, Food and Drug Administration; MRSA, methicillin-resistant Staphylococcus aureus; SSTI, skin and soft tissue infection.

Clindamycin-resistant Streptococcus pyogenes

Unlike S aureus, group A Streptococcus (GAS) remains exquisitely sensitive to penicillin-class antibiotics.

In invasive GAS infections, cotreatment with clindamycin is recommended, but rates of resistance to clindamycin are rising.

Streptococcus pyogenes (GAS) is a gram-positive coccus that causes a variety of infections and immune-mediated sequelae due to antigenic mimicry, interactions with human immunity, and the expression of virulence factors, often with prominent skin findings (Table II ). , 32, 33, 34, 35 Asymptomatic carriage has been reported in 4% to 5% of adults and 2% to 20% of children.

Table II

Spectrum of streptococcal skin infections, treatment recommendations, and notable associated complications33, 34, 35, 36

Disease	Virulence factor	Recommended management (Strength of recommendation, quality of evidence)	Possible Sequelae
Pharyngitis	M-types 1, 3, 5, 6, 12, 14, 17, 19, 24	Rapid antigen detection test or throat culture for confirmation of GAS (strong, high)Oral penicillin V OR amoxicillin OR intramuscular penicillin G (strong, high)If Penicillin-allergic: oral cephalexin OR cefadroxil (strong, high)Other options for penicillin-allergy: oral azithromycin OR erythromycin OR clindamycin (strong, moderate)	Guttate psoriasisAcute rheumatic feverErythema nodosumPolyarteritis nodosaSmall-vessel vasculitisType I sclerodermaAcute febrile neutrophilic dermatosisPeritonsillar abscessCervical lymphadenitisInvasive GAS infection
Acute rheumatic fever†	M-types1, 3, 5, 6, 11, 12, 14, 17, 18, 19, 24, 27, 29, 30, 32, 41	Treatment, regardless of throat culture resultsIM benzathine penicillin OROral penicillin OROral amoxicillin OROral macrolide in penicillin-allergy	Arthritis (60-80%)Carditis∗ (30-45%)Chorea (10%)Erythema marginatum (2%)Subcutaneous nodules
Scarlet fever	M-types 1, 4, 12, 49, 55, 57, 60 (nephritogenic)SPE-A, SPE-B, SPE-C	Oral penicillin or amoxicillinIf penicillin-allergic: 1st generation cephalosporin OR clindamycin OR macrolide	Acute rheumatic fever†Post streptococcal glomerulonephritis when associated with nephritogenic strains
Ecthyma	-	C&S to identify if GAS or S. aureus is the causative pathogen (strong, moderate)Treatment without C&S is reasonable and should include anti-staphylococcal coverage (strong, moderate)Treatment with oral penicillin if GAS identified on culture (strong, high)	-
Impetigo	M-types 33, 41, 42, 52, 53, 70	C&S recommended for pathogen identification (strong, moderate)Treatment without C&S is reasonable and should include anti-staphylococcal coverage (strong, moderate)Localized: Topical mupirocin OR retapamulin (strong, high)Widespread: If GAS identified on culture treat with oral penicillin (strong, high)	If associated with nephritogenic M proteins 1, 4, 12, 49, 55, 57, 60 there is an increased risk of post streptococcal glomerulonephritis
Erysipelas	-	Blood cultures, aspirates, biopsy, or swab not routinely recommended (strong, moderate)If mild with no systemic symptoms outpatient regimen (strong, moderate) with Penicillin OR Cephalosporin OR Dicloxacillin OR Clindamycin	-
Streptococcal toxic shock syndrome (STSS)	M-types 1 and 3Streptolysin OSPE-A > SPE-B, SPE-CAntigen-independent activation of T-cells by SPEs, SSA, SmeZ → massive production of IL-1, IL-6, TNF-α	Early surgical interventionFirst line for documented GAS: Penicillin PLUS ClindamycinLinezolid may be an alternative to clindamycin to inhibit toxin productionIVIG may be used as an adjunct	Widespread tissue damageDisseminated intravascular thrombosisEnd-organ dysfunction
Necrotizing fasciitis	Uncontrolled T-cell response to superantigens → proinflammatory cytokine storm	Prompt surgical consultation (strong, low)Empirical therapy with broad coverage as infection can be poly- or mono-bacterial (strong, low)For documented GAS treat with penicillin PLUS clindamycin (strong, low)	STSSMulti-organ failure

ARF, Acute rheumatic fever; C&S, culture and sensitivity; GAS, group A streptococcus; IL-1, interleukin-1; IL-6, interleukin-6; IVIG, Intravenous immunoglobulins; SmeZ, streptococcal mitogenic exotoxin Z; SPE, streptococcal pyrogenic exotoxin; SSA, streptococcal superantigen; STSS, streptococcal toxic shock syndrome; TNF-α, tumor necrosis factor α.

ARF can still be prevented if antibiotics for GAS pharyngitis are initiated within 9 days of symptom onset.

Rheumatic heart disease is a complication of untreated acute rheumatic fever and is the leading cause of pediatric heart disease worldwide.

GAS most commonly causes pharyngitis and impetigo. Invasive diseases, including bacteremia, cellulitis, necrotizing fasciitis, pneumonia, and puerperal infections, carry mortality rates of 7% to 30% and can be complicated by progression to streptococcal toxic shock syndrome in one-third of the cases, with an associated mortality of up to 81%. ,

Unlike S aureus, GAS is universally sensitive to penicillin, which remains the treatment of choice due to its efficacy, safety, narrow spectrum, and low cost. , , , In invasive GAS infections, however, penicillin monotherapy is associated with higher mortality, and the addition of clindamycin is recommended. The high inoculum of invasive infection means that a bacterial stationary growth phase in which PBPs are relatively underexpressed is reached quickly making penicillin, but not clindamycin, less effective in invasive GAS infection. , , Clindamycin’s ability to inhibit bacterial protein synthesis also helps reduce the GAS toxin production known to complicate these infections. , ,

Since the 1980s, there has been an increase in the incidence and severity of invasive GAS infections. , More than 20% of invasive GAS infections are due to clindamycin- and erythromycin-resistant strains in the United States. Increasing rates of resistance have also been reported in Europe and Asia, with as many as 94% of isolates in China demonstrating clindamycin resistance.38, 39, 40 The rise in clindamycin resistance can be partially attributed to the soaring rates of macrolide resistance over the past 40 years that have paralleled the preferential use of macrolides to treat localized GAS infections. , 41, 42, 43 Since concomitant macrolide and clindamycin resistance arises through the acquisition of erm, the overuse of macrolides for localized GAS infections also promotes clindamycin resistance and jeopardizes the ability of clindamycin to serve as an important therapeutic option for serious, life-threatening GAS infections. Comparatively, linezolid may be an effective alternative to clindamycin, as it provides broad-spectrum, gram-positive coverage and has toxin inhibition properties. ,

Resistant Cutibacterium acnes

Broad-spectrum antibiotic therapy in acne promotes resistance in C acnes and other skin commensals.

C acnes can cause biofilm-associated infections of prosthetic joints, heart valves, and other indwelling implants.

C acnes is a commensal within the pilosebaceous unit thought to contribute to several steps in acne pathogenesis, including follicular occlusion.46, 47, 48, 49 Due to their antibacterial and anti-inflammatory properties, antibiotics are widely used in acne therapy. , Prolonged treatment courses, the bacteriostatic (and not bactericidal) effects of many agents, and the availability of over-the-counter antibiotics in some countries have promoted global C acnes resistance. , ,

C. acnes resistance was first reported in the 1970s, with the introduction and widespread use of topical erythromycin and clindamycin. , , By the late 1990s, global resistance rates had risen to 62%. Approximately 50% of patients with acne develop resistance after oral or topical therapy, and 1 in 4 C acnes strains are currently resistant to tetracyclines, macrolides, or clindamycin. , , C acnes has historically remained sensitive to doxycycline and minocycline, but recent studies demonstrating increasing rates of resistance suggest that this may be changing.54, 55, 56, 57, 58

C acnes resistance mechanisms are tied to the mode of action of specific antimicrobials. Both macrolides and clindamycin inhibit bacterial protein synthesis by binding to the 23S ribosomal RNA (rRNA) subunit. Point mutations and base substitutions in the 23S rRNA binding site confer variable degrees of resistance to clindamycin, macrolides, or both. , , , C acnes can also acquire erm from other C acnes strains or Corynebacterium species, leading to the macrolide-lincosamide-streptogramin B (MLSB) phenotype with high-level macrolide-clindamycin cross-resistance. , Tetracycline resistance occurs with point mutations in the 16S rRNA subunit, affecting antibiotic binding, or via efflux pumps. , , , ,

The development of antibiotic resistance in C acnes is consequential for several reasons. In terms of therapeutic outcomes in acne, resistance has been associated with more recalcitrant lesions and treatment failure.54, 55, 56, 57, 58 , Additionally, resistance complicates the already-challenging treatment of C acnes biofilm-associated infections of prosthetic joints, heart valves, and other indwelling implants. , When broad-spectrum antibiotics, such as doxycycline and clindamycin, are used for acne, their effects can extend beyond C acne s to cause inadvertent damage to “bystander” bacteria of the microbiome. For example, minocycline treatment in acne has been associated with significant dysbiosis of the skin and gut.

Broad-spectrum antibiotic use for acne exerts selection pressure on a wide range of bacteria to promote the survival of strains that have acquired resistance mechanisms. The result is increased resistance among C acnes as well as other pathogenic bacteria such as S aureus, GAS, and coagulase-negative Staphylococcus strains, which compromises the effectiveness of these antibiotics for other treatment indications. For example, clindamycin-resistant coagulase-negative Staphylococcus has been isolated from more than 80% of patients with clindamycin-resistant C acnes. , Furthermore, 35% of patients with acne on antibiotics were found to carry oropharyngeal GAS, 85% of which was tetracycline resistant. The use of clindamycin and doxycycline in acne therapy can also select for resistant MRSA strains, limiting the use of these agents in MRSA infections. , Recent evidence suggests that even low-dose, or subantimicrobial doses of, antibiotics like doxycycline or minocycline may still potentiate resistance through a variety of mechanisms.66, 67, 68

Novel narrow-spectrum antibiotics for acne may help limit resistance. Sarecycline, a tetracyline antibiotic developed for the treatment of acne, maintains high activity against gram-positive bacteria, including C acnes, and demonstrates a low propensity for inducing resistance. Its greatly diminished activity against gram-negative bacteria compared to doxycycline and minocycline allows it to achieve a similar therapeutic efficacy in acne but with a lower risk of gut microbiome and resistome alterations. ,

Multidrug-resistant Pseudomonas aeruginosa

P aeruginosa’s extensive, intrinsic resistance mechanisms make antipseudomonal drug development challenging.

Multidrug-resistant pseudomonal infections are an urgent threat due to the life-threatening nature of invasive infections and very limited treatment options.

P aeruginosa is a ubiquitous, gram-negative, aerobic bacillus implicated in several localized cutaneous and invasive hospital-acquired infections. Life-threatening infections disproportionately affect the immunocompromised and those with extensive skin breakdown.

While localized skin infections have good prognoses, invasive infections have high mortality rates and require prompt and careful antimicrobial selection. , The treatment of a Pseudomonas SSTI typically relies on an antipseudomonal ß-lactam or a fluoroquinolone in combination with surgical intervention. Due to concerns for resistance, the empiric treatment of life-threatening infections involves 2 antipseudomonal agents from different classes while awaiting culture and sensitivities. ,

Owing to intrinsic resistance mechanisms, the available armamentarium against wild-type P aeruginosa includes only a few antibiotic classes (Table III ).69, 70, 71, 72 P aeruginosa has developed resistance mechanisms to all available antipseudomonal antibiotic classes, largely due to the increased use of these agents coupled with the efficient transmission of resistance traits. Since quinolones upregulate and serve as substrates for all 4 known pseudomonas efflux systems, continuous quinolone exposure is an important risk factor for the generation of cross-resistance.

Table III

Antipseudomonal antibiotics and mechanisms of resistance,,,

Antibacterial class	Specific agents	Mechanism of action	Acquired resistance mechanisms
Monobactams	Aztreonam	Binding to PBP3 → inhibition of peptidoglycan synthesis	1) Class A serine ESBLs (PER, VEB, GES, BEL, SHV, CTX-M, TEM) hydrolyze the beta-lactam2) Class D β-lactamases (OXA-type enzymes: OXA-2, OXA-10) hydrolyze the β-lactam
Aminoglycosides	TobramycinGentamicinAmikacinNetilmicin	Binding to 16S rRNA of the 30S ribosomal subunit → inhibition of protein synthesis	1) Aminoglycoside modifying enzymes (AME) inactivate the drug via attachment of acetyl, phosphate or adenyl groups. Most AMEs tend to spare Amikacin2) MexXY-OprM efflux pump reduces drug concentrations3) Genes RMtA or RMtB encoded in mobile genetic elements methylate 16S rRNA leading to an altered drug target
Carbapenem	Imipenem	Binding to PBPs → inhibition of cell-wall synthesis	1) Class D metallo-β-lactamases (IMP, VIM, SPM, GIM) hydrolyze the beta-lactam∗2) MexEF-OprN efflux pump reduces drug concentrations3) Reduced expression of membrane porin OprD leads to decreased drug uptake
	Meropenem		1) Class D metallo-beta-lactamases (IMP, VIM, SPM, GIM) hydrolyze the beta-lactam∗2) MexEF-OprN efflux pump reduces drug concentrations
Cephalosporins	Ceftazidime (3rd generation)Cefepime (4th generation)	Binding to PBPs → inhibition of cell-wall synthesis	1) Class A serine ESBL (PER, VEB, GES, BEL, SHV, CTX-M, TEM) can hydrolyze the beta-lactam2) Class D β-lactamases (OXA-type enzymes: OXA-2, OXA-10) can hydrolyze the beta-lactam
Fluoroquinolones	CiprofloxacinLevofloxacin	Inhibition of enzymes involved in DNA replication DNA gyrase (topoisomerase II) and topoisomerase IV	1) Efflux pumps MexAB-OprM, MexXY-OprM, MexCD-OprJ and MexEF-OprN reduce drug concentrations†2) Point mutations in the quinolone resistance determining region (QRDR) of genes gyrA and gyrB, encoding subunits of the DNA gyrase, and the genes parC and parE, encoding subunits of topoisomerase IV lead to an altered drug target
Penicillins	CarboxipenicillinUreidopenicillin	Binding to penicillin-binding proteins → inhibition of peptidoglycan cross-linking	1) Amber Class A serine β-lactamases of types TEM, PSE or CARB hydrolyze the beta-lactam2) Class A serine ESBL (PER, VEB, GES, BEL, SHV, CTX-M, TEM) hydrolyze the β-lactam3) Class D β-lactamases (OXA-type enzymes:(OXA-1, OXA-2, OXA-10)) hydrolyze the β-lactam
Polymyxins	Colistin and polymyxin B	Binding to lipopolysaccharide and disruption of the cell membrane	1) Enzymatic alteration of the lipopolysaccharide alters drug binding
Fosfomycin	Fosfomycin	Inhibition of MurA enzyme → inhibition of the first step in peptidoglycan synthesis	1) FosA modifying enzyme can lead chemical modification and inactivation of drug2) Mutations in gltP permease, a transporter enzyme, lead to reduced drug uptake

AME, Aminoglycoside modifying enzyme; ESBL, extended spectrum β-lactamase; PBP, penicillin-binding protein.

Class D metallo-β-lactamases (IMP, VIM, SPM, GIM) can hydrolyze all β-lactams, usually except for aztreonam, including the carbapenems and are resistant to β-lactamase inhibitors.,

Four main efflux systems have been described in Pseudomonas: MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY-OprM. The efflux pumps MexAB-OprM, MexXY-OprM can mediate acquired and intrinsic resistance, whereas MexCD-OprJ and MexEF-OprN mediate only acquired resistance.,

The global rise in MDR P aeruginosa, defined as resistance to 1 drug agent in at least 3 classes, has reached rates of 15% to 50%, leaving limited therapeutic options for invasive disease. , , In cases of MDR or pan-resistant strains, last-resort agents include polymyxins (colistin and polymyxin B), which have very narrow therapeutic windows due to nephrotoxicity.

Drug-resistant Mycobacterium tuberculosis

Tuberculosis is difficult to treat, even under ideal conditions.

Several factors have contributed to the global threat of increasingly drug-resistant Mycobacterium tuberculosis (TB).

TB is an acid-fast, alcohol-fast, aerobic bacillus that spreads via airborne transmission and causes more deaths globally than any other infectious agent. Though cutaneous tuberculosis accounts for only 1% of extrapulmonary cases, the past few decades have seen a resurgence of both pulmonary and cutaneous TB and a rise in drug-resistant strains.74, 75, 76, 77

The clinical factors contributing to resistance include an insufficient duration of therapy, nonadherence, interrupted active phase of treatment, lack of access to therapy, and a rise in HIV coinfection. The treatment of drug-susceptible TB requires a 6-month, multidrug treatment with first-line agents and, under ideal conditions, has a success rate of only 85%. The treatment of MDR-TB necessitates more expensive and more toxic second-line agents given for an extended duration, sometimes up to 20 months, to achieve a success rate of 57% (Table IV ).

Table IV

Types of tuberculosis resistance and overview of treatment,,

Resistance	Definition
Drug-resistant tuberculosis	Resistance to 1 of 4 first-line agents, more commonly isoniazid
Multidrug-resistant tuberculosis (MDR-TB)	Resistance to 2 of 4 first-line agents, at least rifampicin and isoniazid
Extensively drug-resistant tuberculosis (XDR-TB)	Resistance to first-line agents (MDR-TB) PLUS at least 1 fluoroquinolone and a second-line injectable agent (amikacin, kanamycin, or capreomycin)
First-line agents	RifampinIsoniazidEthambutolPyrazinamide
Second-line agents	Streptomycin/amikacin/kanamycin, Levofloxacin/moxifloxacin/ofloxacinPara-amino-salicylic acidCapreomycin/viomycinCycloserineTerizidoneEthionamide/Prothionamide
Third line agents	LinezolidClofazimineAmoxicillin-ClavulanateImipenemThiacitazoneClarithromycinBedaquilineDelamanid

Due to the devastating effects of the COVID-19 pandemic on health care resources in lower-income countries, there has been a decline in TB detection and notification and a reallocation of resources away from the TB sector. The World Health Organization estimates that the impact of the pandemic will lead to an additional 6.2 million global TB cases between 2020 and 2025.

Emerging approaches to limit antimicrobial resistance

As demonstrated by the COVID-19 pandemic response, antimicrobial strategies must contend with the relentless ability of microbes to resist and adapt. Conventional antimicrobial drug development has struggled to keep pace with the development of AMR. Simple yet effective resistance reduction strategies include the avoidance of antibiotic monotherapy in acne treatment and the limitation of the duration of oral antibiotic use. Innovative therapies currently being investigated, such as nanoparticles and phage therapy, have actions unlike those of conventional antimicrobials and may be particularly promising strategies to address the AMR crisis.

Phage therapy

Phage therapy utilizes bacterial viruses to (1) selectively attack a bacterial population, (2) deliver drug molecules to a bacterial target, and (3) insert enzymes that inactivate or reverse resistance genes. Given their ubiquitous nature and high specificity for bacterial receptors, bacteriophages can be engineered to target a specific pathogenic bacterial population without damage to collateral flora. ,

Phage therapy is already being applied in the investigational treatment of ulcers, burns, and chronic wounds (Table V ).84, 85, 86, 87, 88, 89 In addition to their specificity, their efficacy against S aureus and Pseudomonas biofilms make bacteriophages an especially promising modality for the management of chronic wounds, ulcers, and acne. C acnes bacteriophages have shown in vitro efficacy in lysing C acnes colonies without affecting collateral microbes, a significant advantage compared to currently used antibacterials. ,

Table V

Clinical trials of dermatologic interest utilizing phage therapy as registered on clinicaltrials.gov

Brief study description	Status	Dermatologic condition	Primary outcome measure	Study identifier
Phase I/II trial comparing the efficacy of standard treatment with a topical anti-staphylococcal bacteriophage cocktail versus placebo for diabetic foot ulcers infected with MRSA or MSSA (127)	Recruitment planned June 2020Aims: 60 adult participants with type 1 or type 2 diabetes and a wound below the ankle (evolving for >2 weeks) mono-infected with MRSA	Diabetic foot ulcers	Relative reduction in wound surface area (%) at 12 weeks.	NCT02664740
Phase I, randomized, open-label study investigating the safety/tolerability of phage-cocktail spray as an adjunct to standard therapy (xeroform dressing and Kenacomb cream for localized signs of infection) for the prevention and treatment of burns susceptible to infection/or infected by S aureus, Paeruginosa, or K pneumoniae	Not yet recruiting	Wound infection	Incidence of treatment-related adverse events and incidence of treatment discontinuation due to adverse events	NCT04323475
Phase I/II, randomized, open-label trial comparing tolerance and efficacy of local bacteriophage treatment (using Pherecydes Pharma anti-Escherichia coli and anti-Pseudomonas aeruginosa bacteriophage cocktails) to standard therapy (silver sulfadiazine) of burn wounds infected with E coli or P aeruginosa	ClosedFindings: The primary endpoint was reached in a median of 144 h (95% CI 48–not reached) in the PP1131 group versus a median of 47 h (23–122) in the standard of care group	Burn wound infection	Median time to sustained reduction of bacterial burden	NCT02116010
Phase I, randomized, controlled double-blind study evaluating the safety of WPP-201 in full thickness venous leg ulcers > 30 days (WPP-201 in a pH neutral phage preparation with 8 bacteriophages lytic for P aeruginosa, S aureus, and E coli)	Completed recruitment	Venous leg ulcers	Safety of product use	NCT00663091
Phase II randomized trial comparing the efficacy of T4N5 liposomal lotion (topical bacteriophage T4 endonuclease V) versus placebo in preventing recurrence of NMSC in patients with previous history of NMSC who have undergone kidney transplant	Completed recruitmentLast update in 2015	Nonmelanoma skin cancer (NMSC)	Incidence of NMSC per patient on the sun-exposed skin of renal transplant recipients with a history of NMSC treated with T4N5 vs placebo	NCT00089180
Phase I randomized, double-blind trial assessing adverse events to 2 ascending concentrations of topical administration of AB-SA01 (topical 3 phage cocktail against S. aureus)	Completed.Application was well tolerated	-	Incidence of adverse events, including change in clinical lab tests from baseline and skin reaction, from first dose through the end of the study, including	NCT02757755

E. coli, Escherichia coli; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible Staphylococcus aureus; NMSC, Nonmelanoma skin cancer; S aureus, Staphylococcus aureus; P aeruginosa, Pseudomonas aeruginosa; K pneumoniae.

Nanoparticles

Nanoparticles are metals or metal oxides that combat microbes via a number of simultaneous mechanisms, including the disruption of the cell membrane, generation of reactive oxygen species, and damage to intracellular contents, making them low-risk for resistance and particularly attractive against MDR pathogens. Nanoparticles hold great potential as protective coatings, such as on wound dressings or implants, to prevent biofilm-associated infections.92, 93, 94

Despite their theoretical and early investigational promise, most of these innovative strategies are still in early stages of development with further investigations needed to establish the safety, in vivo efficacy, interactions with human immunity, and pharmacokinetics before they can be used clinically to address AMR. , 92, 93, 94

Conclusion

Dermatology treatment guidelines issued by expert panels over the past 2 decades consistently emphasize the need for judicious antibiotic use. Overall antibiotic prescribing by dermatologists decreased by 36.6% between 2008 and 2016. During the same time frame, however, oral antibiotic use associated with surgical visits increased by 69.6%, suggesting that there may still be areas for improvement. In the treatment of SSTIs, dermatologists should adhere to evidence-based infectious diseases guidelines for appropriate antibiotic selection (Fig 2 ) and be cognizant of local resistance patterns for key pathogens. Continued support for research into the off-target effects of antibiotics on the cutaneous microbiome and innovative strategies for the treatment of skin disease are needed to generate evidence-based interventions that prevent AMR.

Fig 2

Outpatient management of skin and soft tissue infections from the Infectious Diseases Society of America.

C&S, Culture and sensitivity; I&D, incision and drainage; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible Staphylococcus aureus; TMP/SMX, trimethoprim-sulfamethoxazole; SSTI, skin and soft tissue infection; Rx, treatment.

Conflicts of interest

None disclosed.