Literature DB >> 35112383

Human erythrocyte fragmentation during ex-vivo pig organ perfusion.

Zahra A Habibabady1,2, Selin Sendil1, Felix Ellett3, Franziska Pollok2,4, Gabriela F Elias2, Beth M French1, Wenji Sun1, Gheorghe Braileanu1, Lars Burdorf1,2, Daniel Irimia3, Richard N Pierson1,2, Agnes M Azimzadeh1,2.   

Abstract

Platelet sequestration is a common process during organ reperfusion after transplantation. However, instead of lower platelet counts, when using traditional hemocytometers and light microscopy, we observed physiologically implausible platelet counts in the course of ex-vivo lung and liver xenograft organ perfusion studies. We employed conventional flow cytometry (FC) and imaging FC (AMINS ImageStream X) to investigate the findings and found platelet-sized fragments in the circulation that are mainly derived from red blood cell membranes. We speculate that this erythrocyte fragmentation contributes to anemia during in-vivo organ xenotransplant.
© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Entities:  

Keywords:  RBC fragments; ex-vivo perfusion; flow cytometry; platelets; xenotransplantation

Mesh：

Year:  2022        PMID： 35112383      PMCID： PMC8995366          DOI： 10.1111/xen.12729

Source DB:  PubMed          Journal:  Xenotransplantation        ISSN： 0908-665X            Impact factor:   3.907


  41 in total

1.  An interlaboratory study of a candidate reference method for platelet counting.

Authors:  P Harrison; K A Ault; S Chapman; L Charie; B Davis; K Fujimoto; B Houwen; J Kunicka; F Lacombe; S Machin; R Raynor; L van Hove; O W van Assendelft
Journal:  Am J Clin Pathol       Date:  2001-03       Impact factor: 2.493

2.  Pre-transplant antibody screening and anti-CD154 costimulation blockade promote long-term xenograft survival in a pig-to-primate kidney transplant model.

Authors:  Laura Higginbotham; Dave Mathews; Cynthia A Breeden; Mingqing Song; Alton Brad Farris; Christian P Larsen; Mandy L Ford; Andrew J Lutz; Matthew Tector; Kenneth A Newell; A Joseph Tector; Andrew B Adams
Journal:  Xenotransplantation       Date:  2015-04-03       Impact factor: 3.907

Review 3.  Verification and quality control of routine hematology analyzers.

Authors:  J Y Vis; A Huisman
Journal:  Int J Lab Hematol       Date:  2016-05-09       Impact factor: 2.877

4.  Spurious elevation of automated platelet counts in secondary acute monocytic leukemia associated with tumor lysis syndrome.

Authors:  S Li; K E Salhany
Journal:  Arch Pathol Lab Med       Date:  1999-11       Impact factor: 5.534

5.  Meta-analysis of the independent and cumulative effects of multiple genetic modifications on pig lung xenograft performance during ex vivo perfusion with human blood.

Authors:  Donald G Harris; Kevin J Quinn; Beth M French; Evan Schwartz; Elizabeth Kang; Siamak Dahi; Carol J Phelps; David L Ayares; Lars Burdorf; Agnes M Azimzadeh; Richard N Pierson
Journal:  Xenotransplantation       Date:  2014-12-02       Impact factor: 3.907

6.  Production of alpha 1,3-galactosyltransferase-deficient pigs.

Authors:  Carol J Phelps; Chihiro Koike; Todd D Vaught; Jeremy Boone; Kevin D Wells; Shu-Hung Chen; Suyapa Ball; Susan M Specht; Irina A Polejaeva; Jeff A Monahan; Pete M Jobst; Sugandha B Sharma; Ashley E Lamborn; Amy S Garst; Marilyn Moore; Anthony J Demetris; William A Rudert; Rita Bottino; Suzanne Bertera; Massimo Trucco; Thomas E Starzl; Yifan Dai; David L Ayares
Journal:  Science       Date:  2002-12-19       Impact factor: 47.728

7.  Role of anti-CD40 antibody-mediated costimulation blockade on non-Gal antibody production and heterotopic cardiac xenograft survival in a GTKO.hCD46Tg pig-to-baboon model.

Authors:  Muhammad M Mohiuddin; Avneesh K Singh; Philip C Corcoran; Robert F Hoyt; Marvin L Thomas; Billeta G T Lewis; Michael Eckhaus; Nicole L Dabkowski; Aaron J Belli; Keith A Reimann; David Ayares; Keith A Horvath
Journal:  Xenotransplantation       Date:  2013-10-29       Impact factor: 3.907

8.  N-glycolylneuraminic acid knockout reduces erythrocyte sequestration and thromboxane elaboration in an ex vivo pig-to-human xenoperfusion model.

Authors:  Arielle Cimeno; Wessam Hassanein; Beth M French; Jessica M Powell; Lars Burdorf; Olga Goloubeva; Xiangfei Cheng; Dawn M Parsell; Jagdeece Ramsoondar; Kasinath Kuravi; Todd Vaught; Mehmet C Uluer; Emily Redding; Natalie O'Neill; Christopher Laird; Alena Hershfeld; Ivan Tatarov; Kathryn Thomas; David Ayares; Agnes M Azimzadeh; Richard N Pierson; Rolf N Barth; John C LaMattina
Journal:  Xenotransplantation       Date:  2017-09-22       Impact factor: 3.907

9.  Pig-to-baboon lung xenotransplantation: Extended survival with targeted genetic modifications and pharmacologic treatments.

Authors:  Lars Burdorf; Christopher T Laird; Donald G Harris; Margaret R Connolly; Zahra Habibabady; Emily Redding; Natalie A O'Neill; Arielle Cimeno; Dawn Parsell; Carol Phelps; David Ayares; Agnes M Azimzadeh; Richard N Pierson
Journal:  Am J Transplant       Date:  2021-09-20       Impact factor: 8.086

Review 10.  Imaging Platelet Processes and Function-Current and Emerging Approaches for Imaging in vitro and in vivo.

Authors:  Samantha J Montague; Yean J Lim; Woei M Lee; Elizabeth E Gardiner
Journal:  Front Immunol       Date:  2020-01-31       Impact factor: 7.561
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.