Luis A Chaparro-Encinas1,2, Fannie I Parra-Cota3, Abraham Cruz-Mendívil4, Gustavo Santoyo5, Juan J Peña-Cabriales6, Luciano Castro-Espinoza1, Sergio de Los Santos-Villalobos7. 1. Instituto Tecnológico de Sonora, 5 de febrero 818 Sur, C.P. 85000, Col. Centro, Ciudad Obregón, Sonora, México. 2. Universidad Autónoma Agraria Antonio Narro (UAAAN) Unidad Laguna, Periférico Raúl López Sánchez, Valle Verde, 27054, Torreón, Coahuila, México. 3. Campo Experimental Norman E. Borlaug-CIRNO. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Norman E. Borlaug Km. 12, CP 85000, Valle del Yaqui, Ciudad Obregón, Sonora, México. 4. Cátedras CONACYT, Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación Para el Desarrollo Integral Regional (CIIDIR) Unidad Sinaloa, Guasave, Sinaloa, México. 5. Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México. 6. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Campus Guanajuato, Irapuato Guanajuato, México. 7. Instituto Tecnológico de Sonora, 5 de febrero 818 Sur, C.P. 85000, Col. Centro, Ciudad Obregón, Sonora, México. sergio.delossantos@itson.edu.mx.
Abstract
MAIN CONCLUSIONS: Bacillus paralicheniformis TRQ65 reprograms the gene expression patterns associated with systemic response to potentially facilitate its colonization and stimulate cell growth and plant biomass. Plant growth-promoting rhizobacteria (PGPR) carry out numerous mechanisms that enhance growth in seedlings, such as nutrient solubilization, phytohormone production, biocontrol activity, and regulation of induced systemic resistance (ISR) and acquired systemic resistance (ASR). Bacillus paralicheniformis TRQ65 is a biological and plant growth-promoting bacterium isolated from wheat (Triticum turgidum subsp. durum) rhizosphere. In this study, we performed a transcriptomic analysis of wheat seedlings inoculated with the native rhizobacterium Bacillus paralicheniformis TRQ65 (1 × 107 cells∙g -1 of soil) at early development stages (GS15). A morphometrical assay was carried out to confirm growth promotion and after the cultivation period, TRQ65 was re-isolated to define inoculum persistence. Inoculated seedlings showed a significant (P < 0.05) increase in shoot length (93.48%) and dry weight in both shoot (117.02%) and root (48.33%) tissues; also, the strain persisted in the soil at 1.4 × 107 UFC∙g-1 of soil. A total of 228 differentially expressed genes (DEGs) (FDR < 0.05 and |log2 fold change|≥ 1.3) were observed in response to TRQ65 inoculation, of which 185 were down-regulated and 43 were up-regulated. The transcriptional patterns were characterized by the regulation of multidimensional cell growth (ROS, Ca+2 channel, and NADPH oxidases activity), suppression of defense mechanism (PR proteins, PDFs, ROS, transcription factors), induction of central stimuli receptors (RALF, WAK, MAPK), carbohydrate metabolism (invertase activity) and phytohormone-related transport (ABCG transporter and AAAP). These results suggest that B. paralicheniformis TRQ65 is a promising bioinoculant agent for increasing wheat growth and development by reprogramming ISR and ASR simultaneously, suppressing defense mechanisms and inducing central stimuli response.
MAIN CONCLUSIONS: Bacillus paralicheniformis TRQ65 reprograms the gene expression patterns associated with systemic response to potentially facilitate its colonization and stimulate cell growth and plant biomass. Plant growth-promoting rhizobacteria (PGPR) carry out numerous mechanisms that enhance growth in seedlings, such as nutrient solubilization, phytohormone production, biocontrol activity, and regulation of induced systemic resistance (ISR) and acquired systemic resistance (ASR). Bacillus paralicheniformis TRQ65 is a biological and plant growth-promoting bacterium isolated from wheat (Triticum turgidum subsp. durum) rhizosphere. In this study, we performed a transcriptomic analysis of wheat seedlings inoculated with the native rhizobacterium Bacillus paralicheniformis TRQ65 (1 × 107 cells∙g -1 of soil) at early development stages (GS15). A morphometrical assay was carried out to confirm growth promotion and after the cultivation period, TRQ65 was re-isolated to define inoculum persistence. Inoculated seedlings showed a significant (P < 0.05) increase in shoot length (93.48%) and dry weight in both shoot (117.02%) and root (48.33%) tissues; also, the strain persisted in the soil at 1.4 × 107 UFC∙g-1 of soil. A total of 228 differentially expressed genes (DEGs) (FDR < 0.05 and |log2 fold change|≥ 1.3) were observed in response to TRQ65 inoculation, of which 185 were down-regulated and 43 were up-regulated. The transcriptional patterns were characterized by the regulation of multidimensional cell growth (ROS, Ca+2 channel, and NADPH oxidases activity), suppression of defense mechanism (PR proteins, PDFs, ROS, transcription factors), induction of central stimuli receptors (RALF, WAK, MAPK), carbohydrate metabolism (invertase activity) and phytohormone-related transport (ABCG transporter and AAAP). These results suggest that B. paralicheniformis TRQ65 is a promising bioinoculant agent for increasing wheat growth and development by reprogramming ISR and ASR simultaneously, suppressing defense mechanisms and inducing central stimuli response.
Authors: Alondra M Díaz Rodríguez; Fannie I Parra Cota; Gustavo Santoyo; Sergio de Los Santos Villalobos Journal: Ecotoxicology Date: 2019-05-25 Impact factor: 2.823
Authors: Raz Avni; Moran Nave; Omer Barad; Kobi Baruch; Sven O Twardziok; Heidrun Gundlach; Iago Hale; Martin Mascher; Manuel Spannagl; Krystalee Wiebe; Katherine W Jordan; Guy Golan; Jasline Deek; Batsheva Ben-Zvi; Gil Ben-Zvi; Axel Himmelbach; Ron P MacLachlan; Andrew G Sharpe; Allan Fritz; Roi Ben-David; Hikmet Budak; Tzion Fahima; Abraham Korol; Justin D Faris; Alvaro Hernandez; Mark A Mikel; Avraham A Levy; Brian Steffenson; Marco Maccaferri; Roberto Tuberosa; Luigi Cattivelli; Primetta Faccioli; Aldo Ceriotti; Khalil Kashkush; Mohammad Pourkheirandish; Takao Komatsuda; Tamar Eilam; Hanan Sela; Amir Sharon; Nir Ohad; Daniel A Chamovitz; Klaus F X Mayer; Nils Stein; Gil Ronen; Zvi Peleg; Curtis J Pozniak; Eduard D Akhunov; Assaf Distelfeld Journal: Science Date: 2017-07-07 Impact factor: 47.728