Sukhdev Singh1,2, Kuleshwar Sahu1,2, Lakshay Kapil1,2, Charan Singh3,2, Arti Singh4,5. 1. Department of Pharmacology, ISF College of Pharmacy, Moga, 142001, Punjab, India. 2. Affiliated to IK Gujral Punjab Technical University, Jalandhar, 144603, Punjab, India. 3. Department of Pharmaceutics, ISF College of Pharmacy, Moga, 142001, Punjab, India. 4. Department of Pharmacology, ISF College of Pharmacy, Moga, 142001, Punjab, India. artiniper@gmail.com. 5. Affiliated to IK Gujral Punjab Technical University, Jalandhar, 144603, Punjab, India. artiniper@gmail.com.
Abstract
BACKGROUND: Quercetin is a natural flavonoid that is known to have numerous pharmacological activities such as antioxidative, anti-inflammatory, and neuroprotective effects against various neurological disorders. Lipopolysaccharide (LPS) is a potent endotoxin, reported causing several neurological disorders. AIM: The present study was designed to investigate the possibility that quercetin ameliorates LPS induced oxidative stress and neuroinflammation in adult zebrafish. MATERIALS AND METHODS: Zebrafish (weighing 470-530 mg) were treated with a single injection of LPS (1 mg/kg) intraperitoneally (i.p.) followed by post-treatment with quercetin (50 and 100 mg/kg; i.p.) for 7 days. After sacrificing brain was harvested and subjected for biochemical, molecular, and histological analyses. RESULTS: Results revealed post-treatment with quercetin was able to ameliorate the behavioral abnormalities as in novel tank diving test- time spent in the top zone (TSTZ), and the number of entries in the top zone was significantly (p < 0.01) more as compared to time spent in the bottom zone (TSBZ). In the light-dark chamber test- time spent in the light zone (TSLZ), and the number of entries in the light zone were significantly (p < 0.01) more as compared to time spent in the dark compartment (TSDC). Additionally, results of histopathology (H & E stain) studies showed less disruption in neuronal cells as compared to the LPS treated group. Moreover, the results of the molecular analysis revealed that quercetin treatment significantly (p < 0.01) decrease TNF-α and IL-1β levels as compared to LPS treated animals. Further, results of the biochemical analysis reveal that quercetin significantly (p < 0.01) reduces the level of LPO, nitrite, AChEs and increases anti-oxidant GSH. CONCLUSION: Quercetin treatment helps to prevent oxidative damage and neuroinflammation in LPS treated adult zebrafish.
BACKGROUND: Quercetin is a natural flavonoid that is known to have numerous pharmacological activities such as antioxidative, anti-inflammatory, and neuroprotective effects against various neurological disorders. Lipopolysaccharide (LPS) is a potent endotoxin, reported causing several neurological disorders. AIM: The present study was designed to investigate the possibility that quercetin ameliorates LPS induced oxidative stress and neuroinflammation in adult zebrafish. MATERIALS AND METHODS: Zebrafish (weighing 470-530 mg) were treated with a single injection of LPS (1 mg/kg) intraperitoneally (i.p.) followed by post-treatment with quercetin (50 and 100 mg/kg; i.p.) for 7 days. After sacrificing brain was harvested and subjected for biochemical, molecular, and histological analyses. RESULTS: Results revealed post-treatment with quercetin was able to ameliorate the behavioral abnormalities as in novel tank diving test- time spent in the top zone (TSTZ), and the number of entries in the top zone was significantly (p < 0.01) more as compared to time spent in the bottom zone (TSBZ). In the light-dark chamber test- time spent in the light zone (TSLZ), and the number of entries in the light zone were significantly (p < 0.01) more as compared to time spent in the dark compartment (TSDC). Additionally, results of histopathology (H & E stain) studies showed less disruption in neuronal cells as compared to the LPS treated group. Moreover, the results of the molecular analysis revealed that quercetin treatment significantly (p < 0.01) decrease TNF-α and IL-1β levels as compared to LPS treated animals. Further, results of the biochemical analysis reveal that quercetin significantly (p < 0.01) reduces the level of LPO, nitrite, AChEs and increases anti-oxidant GSH. CONCLUSION: Quercetin treatment helps to prevent oxidative damage and neuroinflammation in LPS treated adult zebrafish.
Authors: Paul Browne; Dhia Chandraratna; Ceri Angood; Helen Tremlett; Chris Baker; Bruce V Taylor; Alan J Thompson Journal: Neurology Date: 2014-09-09 Impact factor: 9.910
Authors: Kerstin Howe; Matthew D Clark; Carlos F Torroja; James Torrance; Camille Berthelot; Matthieu Muffato; John E Collins; Sean Humphray; Karen McLaren; Lucy Matthews; Stuart McLaren; Ian Sealy; Mario Caccamo; Carol Churcher; Carol Scott; Jeffrey C Barrett; Romke Koch; Gerd-Jörg Rauch; Simon White; William Chow; Britt Kilian; Leonor T Quintais; José A Guerra-Assunção; Yi Zhou; Yong Gu; Jennifer Yen; Jan-Hinnerk Vogel; Tina Eyre; Seth Redmond; Ruby Banerjee; Jianxiang Chi; Beiyuan Fu; Elizabeth Langley; Sean F Maguire; Gavin K Laird; David Lloyd; Emma Kenyon; Sarah Donaldson; Harminder Sehra; Jeff Almeida-King; Jane Loveland; Stephen Trevanion; Matt Jones; Mike Quail; Dave Willey; Adrienne Hunt; John Burton; Sarah Sims; Kirsten McLay; Bob Plumb; Joy Davis; Chris Clee; Karen Oliver; Richard Clark; Clare Riddle; David Elliot; David Eliott; Glen Threadgold; Glenn Harden; Darren Ware; Sharmin Begum; Beverley Mortimore; Beverly Mortimer; Giselle Kerry; Paul Heath; Benjamin Phillimore; Alan Tracey; Nicole Corby; Matthew Dunn; Christopher Johnson; Jonathan Wood; Susan Clark; Sarah Pelan; Guy Griffiths; Michelle Smith; Rebecca Glithero; Philip Howden; Nicholas Barker; Christine Lloyd; Christopher Stevens; Joanna Harley; Karen Holt; Georgios Panagiotidis; Jamieson Lovell; Helen Beasley; Carl Henderson; Daria Gordon; Katherine Auger; Deborah Wright; Joanna Collins; Claire Raisen; Lauren Dyer; Kenric Leung; Lauren Robertson; Kirsty Ambridge; Daniel Leongamornlert; Sarah McGuire; Ruth Gilderthorp; Coline Griffiths; Deepa Manthravadi; Sarah Nichol; Gary Barker; Siobhan Whitehead; Michael Kay; Jacqueline Brown; Clare Murnane; Emma Gray; Matthew Humphries; Neil Sycamore; Darren Barker; David Saunders; Justene Wallis; Anne Babbage; Sian Hammond; Maryam Mashreghi-Mohammadi; Lucy Barr; Sancha Martin; Paul Wray; Andrew Ellington; Nicholas Matthews; Matthew Ellwood; Rebecca Woodmansey; Graham Clark; James D Cooper; James Cooper; Anthony Tromans; Darren Grafham; Carl Skuce; Richard Pandian; Robert Andrews; Elliot Harrison; Andrew Kimberley; Jane Garnett; Nigel Fosker; Rebekah Hall; Patrick Garner; Daniel Kelly; Christine Bird; Sophie Palmer; Ines Gehring; Andrea Berger; Christopher M Dooley; Zübeyde Ersan-Ürün; Cigdem Eser; Horst Geiger; Maria Geisler; Lena Karotki; Anette Kirn; Judith Konantz; Martina Konantz; Martina Oberländer; Silke Rudolph-Geiger; Mathias Teucke; Christa Lanz; Günter Raddatz; Kazutoyo Osoegawa; Baoli Zhu; Amanda Rapp; Sara Widaa; Cordelia Langford; Fengtang Yang; Stephan C Schuster; Nigel P Carter; Jennifer Harrow; Zemin Ning; Javier Herrero; Steve M J Searle; Anton Enright; Robert Geisler; Ronald H A Plasterk; Charles Lee; Monte Westerfield; Pieter J de Jong; Leonard I Zon; John H Postlethwait; Christiane Nüsslein-Volhard; Tim J P Hubbard; Hugues Roest Crollius; Jane Rogers; Derek L Stemple Journal: Nature Date: 2013-04-17 Impact factor: 49.962
Authors: A Chiò; G Logroscino; B J Traynor; J Collins; J C Simeone; L A Goldstein; L A White Journal: Neuroepidemiology Date: 2013-07-11 Impact factor: 3.282
Authors: Yvonne M Bradford; Sabrina Toro; Sridhar Ramachandran; Leyla Ruzicka; Douglas G Howe; Anne Eagle; Patrick Kalita; Ryan Martin; Sierra A Taylor Moxon; Kevin Schaper; Monte Westerfield Journal: ILAR J Date: 2017-07-01