| Literature DB >> 35089571 |
Joaquin Seras-Franzoso1,2, Olivia Cano-Garrido3,2,4, Spela Peternel5, Anna Arís6, Elena Garcia-Fruitós7.
Abstract
Purification of inclusion bodies (IBs) is gaining importance due to the raising of novel applications for these submicron particulate protein clusters, with potential uses in the biomedical and biotechnological fields among others. Here, we present five optimized methods to purify IBs adapting classical procedures to the material nature, as well as the requirements of the producer cell (Gram-negative bacteria, Gram-positive bacteria, or yeast) and the IB final application.Entities:
Keywords: Bacterial cell free; Cell disruption; Escherichia coli; Inclusion Body; Lactococcus lactis; Nanoparticles; Pichia pastoris; Purification
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Year: 2022 PMID： 35089571 DOI： 10.1007/978-1-0716-1859-2_24
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745