| Literature DB >> 350879 |
S M Panasenko, R J Alazard, I R Lehman.
Abstract
A simple three-step procedure for the large scale purification of DNA ligase has been developed. THe source of enzyme is a strain of Escherichia coli with a hybrid lambda prophage constructed in vitro that bears the ligase overproducing gene lop 11 lig+ (Panasenko, S., Cameron, J., Davis, R. W., and Lehman, I. R. (1977) Science 196, 188-189). The procedure yields homogeneous enzyme in approximately 40% yield.Entities:
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Year: 1978 PMID： 350879
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157