Literature DB >> 3507387

Analysis of closely related genes by the use of synthetic oligonucleotide probes labeled to a high specific activity.

L Hellman1, U Pettersson.   

Abstract

A method for labeling synthetic oligonucleotide probes to high specific activity is described. The method utilizes two partly complementary oligonucleotides that are labeled by a fill-in reaction using the Klenow fragment of DNA polymerase I and four alpha 32P-nucleoside triphosphates. Such probes can, in combination with Southern blot analysis, be used for routine analysis of individual genes in multigene families.

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Year:  1987        PMID： 3507387     DOI： 10.1016/0735-0651(87)90007-0

Source DB:  PubMed          Journal:  Gene Anal Tech        ISSN： 0735-0651


  6 in total

Review 1.  In situ hybridization of messenger RNA sequences.

Authors:  L I Larsson
Journal:  Histochem J       Date:  1989-08

2.  Human satellite-III DNA: an example of a "macrosatellite" polymorphism.

Authors:  C Fowler; R Drinkwater; J Skinner; L Burgoyne
Journal:  Hum Genet       Date:  1988-07       Impact factor: 4.132

3.  Immunoglobulin light chain variable region gene sequences for human antibodies to Haemophilus influenzae type b capsular polysaccharide are dominated by a limited number of V kappa and V lambda segments and VJ combinations.

Authors:  E E Adderson; P G Shackelford; R A Insel; A Quinn; P M Wilson; W L Carroll
Journal:  J Clin Invest       Date:  1992-03       Impact factor: 14.808

4.  Presence of a 16S rRNA pseudogene in the bi-molecular plastid genome of the primitive brown alga Pylaiella littoralis. Evolutionary implications.

Authors:  Y Markowicz; S Loiseaux-de Goër; R Mache
Journal:  Curr Genet       Date:  1988-12       Impact factor: 3.886

5.  Cell proteins bind to sites within the 3' noncoding region and the positive-strand leader sequence of measles virus RNA.

Authors:  R Leopardi; V Hukkanen; R Vainionpää; A A Salmi
Journal:  J Virol       Date:  1993-02       Impact factor: 5.103

6.  Molecular cloning of the major surface antigen of leishmania.

Authors:  L L Button; W R McMaster
Journal:  J Exp Med       Date:  1988-02-01       Impact factor: 14.307
  6 in total

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