Haimei Chen1, Zhuoer Chen2, Qing Du3, Mei Jiang1, Bin Wang2, Chang Liu4. 1. Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, 100193, Beijing, People's Republic of China. 2. School of Pharmacy, Xiangnan University, 423000, Chenzhou, Hunan, China. 3. College of Pharmacy, Qinghai Minzu University, 810007, Xining, Qinghai, China. 4. Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, 100193, Beijing, People's Republic of China. cliu6688@yahoo.com.
Abstract
BACKGROUND: Plants belonging to the Bignoniaceae family have a wide distribution in the tropics and large populations around the world. However, limited information is available about Bignoniaceae. This study aimed to obtain more research information about Bignoniaceae plants and provide data support for the study of plant plastid genomes. METHODS AND RESULTS: In the present study, we focused on the chloroplast genome bio-information of Campsis grandiflora. The chloroplast DNA of C. grandiflora was extracted, sequenced, assembled, and annotated with corresponding software. Results show that the complete chloroplast genome of C. grandiflora is 154,303 bp in length and has a quadripartite structure with large single copy of 85,064 bp and a small single copy of 18,009 bp separated by inverted repeats of 25,615 bp. A total of 110 genes in C. grandiflora comprised 79 protein-coding genes, 27 transfer RNA genes, and 4 ribosomal RNA genes. The distribution of simple sequence repeats and long repeat sequences was determined. We carried out phylogenetic analysis based on homologous amino acid sequence among 45 species derived from Bignoniaceae. Compared with the chloroplast genome of A. thaliana, an inversion was identified in that of C. grandiflora, which result in the incomplete clpP gene. CONCLUSIONS: The chloroplast genomes were used for molecular marker, species identification, and phylogenetic studies. The outcome strongly supported that C. grandiflora and genus Incarvillea formed a cluster within Bignoniaceae. This study identified the unique characteristics of the C. grandiflora cp. genome, thus providing theoretical basis for species identification and biological research.
BACKGROUND: Plants belonging to the Bignoniaceae family have a wide distribution in the tropics and large populations around the world. However, limited information is available about Bignoniaceae. This study aimed to obtain more research information about Bignoniaceae plants and provide data support for the study of plant plastid genomes. METHODS AND RESULTS: In the present study, we focused on the chloroplast genome bio-information of Campsis grandiflora. The chloroplast DNA of C. grandiflora was extracted, sequenced, assembled, and annotated with corresponding software. Results show that the complete chloroplast genome of C. grandiflora is 154,303 bp in length and has a quadripartite structure with large single copy of 85,064 bp and a small single copy of 18,009 bp separated by inverted repeats of 25,615 bp. A total of 110 genes in C. grandiflora comprised 79 protein-coding genes, 27 transfer RNA genes, and 4 ribosomal RNA genes. The distribution of simple sequence repeats and long repeat sequences was determined. We carried out phylogenetic analysis based on homologous amino acid sequence among 45 species derived from Bignoniaceae. Compared with the chloroplast genome of A. thaliana, an inversion was identified in that of C. grandiflora, which result in the incomplete clpP gene. CONCLUSIONS: The chloroplast genomes were used for molecular marker, species identification, and phylogenetic studies. The outcome strongly supported that C. grandiflora and genus Incarvillea formed a cluster within Bignoniaceae. This study identified the unique characteristics of the C. grandiflora cp. genome, thus providing theoretical basis for species identification and biological research.
Authors: Conrad L Schoch; Stacy Ciufo; Mikhail Domrachev; Carol L Hotton; Sivakumar Kannan; Rogneda Khovanskaya; Detlef Leipe; Richard Mcveigh; Kathleen O'Neill; Barbara Robbertse; Shobha Sharma; Vladimir Soussov; John P Sullivan; Lu Sun; Seán Turner; Ilene Karsch-Mizrachi Journal: Database (Oxford) Date: 2020-01-01 Impact factor: 3.451