Literature DB >> 3501569

Determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids by reverse-phase high pressure liquid chromatography.

E H Wiltink1, P Stekkinger, J A Brakenhoff, S A Danner.   

Abstract

In this article a simple method for the determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids is presented, based on reversed-phase ion-pair HPLC with UV detection at 254 nm. No complicated extraction procedure is needed. The stationary phase consists of a reversed-phase C18 stainless steel column and the mobile phase of 0.005 M sodium acetate and 0.0025 M pentanesulfonic acid sodium salt (PIC-B5) as an ion-pair reagent in water (pH 6.5). DHPG is a new antiviral drug in research of the treatment of cytomegalovirus infections. As a pilot study serum and urine samples of a few patients were investigated to gain an impression of the therapeutic range of DHPG. The method is suitable for routine assay of DHPG.

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Year:  1987        PMID： 3501569     DOI： 10.1007/BF01953628

Source DB:  PubMed          Journal:  Pharm Weekbl Sci        ISSN： 0167-6555


  10 in total

1.  Disinfection and inactivation of the human T lymphotropic virus type III/Lymphadenopathy-associated virus.

Authors:  L S Martin; J S McDougal; S L Loskoski
Journal:  J Infect Dis       Date:  1985-08       Impact factor: 5.226

2.  9-(1,3-Dihydroxy-2-propoxymethyl)guanine for cytomegalovirus infections in patients with the acquired immunodeficiency syndrome.

Authors:  M C Bach; S P Bagwell; N P Knapp; K M Davis; P S Hedstrom
Journal:  Ann Intern Med       Date:  1985-09       Impact factor: 25.391

3.  Prolonged inhibitory effect of 9-(1,3-dihydroxy-2-propoxymethyl)guanine against replication of Epstein-Barr virus.

Authors:  J C Lin; M C Smith; J S Pagano
Journal:  J Virol       Date:  1984-04       Impact factor: 5.103

Review 4.  Acyclovir: an update of the clinical applications of this antiherpes agent.

Authors:  A P Fiddian; D Brigden; J M Yeo; E A Hickmott
Journal:  Antiviral Res       Date:  1984-06       Impact factor: 5.970

5.  Simple high-performance liquid chromatographic method for the analysis of 9-(2-hydroxyethoxymethyl)guanine (acyclovir) in human plasma and urine.

Authors:  G Land; A Bye
Journal:  J Chromatogr       Date:  1981-06-12

6.  A dual-column HPLC method for the simultaneous determination of DHPG (9-[(1,3-dihydroxy-2-propoxy)methyl]guanine) and its mono and diesters in biological samples.

Authors:  E J Benjamin; B A Firestone; J A Schneider
Journal:  J Chromatogr Sci       Date:  1985-04       Impact factor: 1.618

Review 7.  Acyclovir. A review of its pharmacodynamic properties and therapeutic efficacy.

Authors:  D M Richards; A A Carmine; R N Brogden; R C Heel; T M Speight; G S Avery
Journal:  Drugs       Date:  1983-11       Impact factor: 9.546

8.  Effect of 9-(1,3-dihydroxy-2-propoxymethyl)guanine on human cytomegalovirus replication in vitro.

Authors:  E C Mar; Y C Cheng; E S Huang
Journal:  Antimicrob Agents Chemother       Date:  1983-10       Impact factor: 5.191

9.  Activity of 9-[2-hydroxy-1-(hydroxymethyl)ethoxymethyl]guanine in the treatment of cytomegalovirus pneumonia.

Authors:  D H Shepp; P S Dandliker; P de Miranda; T C Burnette; D M Cederberg; L E Kirk; J D Meyers
Journal:  Ann Intern Med       Date:  1985-09       Impact factor: 25.391

10.  9-[(1,3-Dihydroxy-2-propoxy)methyl]guanine: a new potent and selective antiherpes agent.

Authors:  J C Martin; C A Dvorak; D F Smee; T R Matthews; J P Verheyden
Journal:  J Med Chem       Date:  1983-05       Impact factor: 7.446
  10 in total
  1 in total

1.  Preparation and ocular pharmacokinetics of ganciclovir liposomes.

Authors:  Yan Shen; Jiasheng Tu
Journal:  AAPS J       Date:  2007-12-07       Impact factor: 4.009
  1 in total

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