Literature DB >> 3499950

Inositol trisphosphate-induced calcium release in brain microsomes.

J Shah1, R S Cohen, H C Pant.   

Abstract

The effects of different ionic media on Ca2+ uptake and release in isolated brain microsomes were investigated. KCl (100 mM) provided the best medium for Ca2+ uptake in the presence of ATP. The effect of myo-inositol 1,4,5-trisphosphate (IP3) on Ca2+ release was examined and was maximum at 0.2 microM. IP3-induced Ca2+ release was dependent on extramicrosomal free Ca2+ concentration with maximal release at 5.0 microM free Ca2+. Replacement of KCl by sucrose or NaCl did not show any response to IP3. Electron microscopy showed that the microsomal fraction consisted of characteristic endoplasmic reticulum-derived vesicular profiles and were free of mitochondria or plasma membrane contamination. Our results support the concept that the endoplasmic reticulum is the target for IP-3 induced mobilization of Ca2+ in the cell.

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Year:  1987        PMID： 3499950     DOI： 10.1016/0006-8993(87)90562-2

Source DB:  PubMed          Journal:  Brain Res        ISSN： 0006-8993            Impact factor:   3.252


  7 in total

1.  The effect of external calcium and pH on inositol trisphosphate-mediated calcium release from cerebellum microsomal fractions.

Authors:  S K Joseph; H L Rice; J R Williamson
Journal:  Biochem J       Date:  1989-02-15       Impact factor: 3.857

2.  Physiological concentrations of inorganic phosphate affect MgATP-dependent Ca2+ storage and inositol trisphosphate-induced Ca2+ efflux in microsomal vesicles from non-hepatic cells.

Authors:  R Fulceri; G Bellomo; A Gamberucci; A Romani; A Benedetti
Journal:  Biochem J       Date:  1993-01-01       Impact factor: 3.857

3.  Inositol 1,4,5-trisphosphate binding sites in the brain: regional distribution, characterization, and alterations in brains of patients with Parkinson's disease.

Authors:  N Kitamura; T Hashimoto; N Nishino; C Tanaka
Journal:  J Mol Neurosci       Date:  1989       Impact factor: 3.444

4.  Ca2+ release by inositol 1,4,5-trisphosphate is blocked by the K(+)-channel blockers apamin and tetrapentylammonium ion, and a monoclonal antibody to a 63 kDa membrane protein: reversal of blockade by K+ ionophores nigericin and valinomycin and purification of the 63 kDa antibody-binding protein.

Authors:  F O'Rourke; K Soons; R Flaumenhauft; J Watras; C Baio-Larue; E Matthews; M B Feinstein
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857

5.  Potassium-channel blockers inhibit inositol trisphosphate-induced calcium release in the microsomal fractions isolated from the rat brain.

Authors:  J Shah; H C Pant
Journal:  Biochem J       Date:  1988-03-01       Impact factor: 3.857

6.  A cerebellar Purkinje cell marker P400 protein is an inositol 1,4,5-trisphosphate (InsP3) receptor protein. Purification and characterization of InsP3 receptor complex.

Authors:  N Maeda; M Niinobe; K Mikoshiba
Journal:  EMBO J       Date:  1990-01       Impact factor: 11.598

7.  Inositol 1,4,5-trisphosphate-induced calcium release in the organelle layers of the stratified, intact egg of Xenopus laevis.

Authors:  J K Han; R Nuccitelli
Journal:  J Cell Biol       Date:  1990-04       Impact factor: 10.539
  7 in total

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