Literature DB >> 3499141

Metabolism of inositol 1,4,5-trisphosphate in permeabilized rat aortic smooth-muscle cells. Dependence on calcium concentration.

M F Rossier1, A M Capponi, M B Vallotton.   

Abstract

The metabolism of [3H]inositol 1,4,5-trisphosphate ([3H]Ins(1,4,5)P3) was studied in permeabilized rat aortic smooth-muscle cells. Addition of [3H]Ins(1,4,5)P3 to the leaky cells led to formation of several labelled metabolites. Amounts of [3H]inositol bisphosphate and [3H]inositol 1,3,4,5-tetrakisphosphate ([3H]InsP4) reached a maximum within 2 min of incubation, whereas production of [3H]inositol monophosphate and [3H]inositol 1,3,4-trisphosphate ([3H]Ins(1,3,4)P3) was delayed. Formation of InsP4 and Ins(1,3,4)P3 was Ca2+-sensitive in the physiological intracellular range (0.06-5 microM), showing a maximum at 1 microM-Ca2+. A correlation between the formation of InsP4 and that of Ins(1,3,4)P3 was observed, suggesting that the former is the precursor of the latter. These results suggest that, in vascular smooth-muscle cells, Ins(1,4,5)P3 is metabolized via two distinct pathways: (1) a dephosphorylation pathway, leading to formation of inositol bis- and mono-phosphate; and (2) a Ca2+-sensitive phosphorylation/dephosphorylation pathway, involving formation of InsP4 and leading to formation of Ins(1,3,4)P3.

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Year:  1987        PMID： 3499141      PMCID： PMC1148118          DOI： 10.1042/bj2450305

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  Rapid formation of inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands may both result indirectly from receptor-stimulated release of inositol 1,4,5-trisphosphate from phosphatidylinositol 4,5-bisphosphate.

Authors:  P T Hawkins; L Stephens; C P Downes
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

2.  The inositol tris/tetrakisphosphate pathway--demonstration of Ins(1,4,5)P3 3-kinase activity in animal tissues.

Authors:  R F Irvine; A J Letcher; J P Heslop; M J Berridge
Journal:  Nature       Date:  1986 Apr 17-23       Impact factor: 49.962

3.  Protein kinase C phosphorylates human platelet inositol trisphosphate 5'-phosphomonoesterase, increasing the phosphatase activity.

Authors:  T M Connolly; W J Lawing; P W Majerus
Journal:  Cell       Date:  1986-09-12       Impact factor: 41.582

4.  Ca2+ regulates the inositol tris/tetrakisphosphate pathway in intact and broken preparations of insulin-secreting RINm5F cells.

Authors:  T J Biden; C B Wollheim
Journal:  J Biol Chem       Date:  1986-09-15       Impact factor: 5.157

5.  Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.

Authors:  I R Batty; S R Nahorski; R F Irvine
Journal:  Biochem J       Date:  1985-11-15       Impact factor: 3.857

6.  Glucose-induced accumulation of inositol trisphosphates in isolated pancreatic islets. Predominance of the 1,3,4-isomer.

Authors:  J Turk; B A Wolf; M L McDaniel
Journal:  Biochem J       Date:  1986-07-01       Impact factor: 3.857

7.  Interconversion of inositol (1,4,5)-trisphosphate to inositol (1,3,4,5)-tetrakisphosphate and (1,3,4)-trisphosphate in permeabilized adrenal glomerulosa cells is calcium-sensitive and ATP-dependent.

Authors:  M F Rossier; I A Dentand; P D Lew; A M Capponi; M B Vallotton
Journal:  Biochem Biophys Res Commun       Date:  1986-08-29       Impact factor: 3.575

8.  The role of cytosolic free calcium in the generation of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in HL-60 cells. Differential effects of chemotactic peptide receptor stimulation at distinct Ca2+ levels.

Authors:  P D Lew; A Monod; K H Krause; F A Waldvogel; T J Biden; W Schlegel
Journal:  J Biol Chem       Date:  1986-10-05       Impact factor: 5.157

9.  Phorbol 12,13-dibutyrate and 1-oleyl-2-acetyldiacylglycerol stimulate inositol trisphosphate dephosphorylation in human platelets.

Authors:  L M Molina y Vedia; E G Lapetina
Journal:  J Biol Chem       Date:  1986-08-15       Impact factor: 5.157

10.  Inositol 1,3,4,5-tetrakisphosphate and not phosphatidylinositol 3,4-bisphosphate is the probable precursor of inositol 1,3,4-trisphosphate in agonist-stimulated parotid gland.

Authors:  C P Downes; P T Hawkins; R F Irvine
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857
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  6 in total

1.  Functional shift from muscarinic to nicotinic cholinergic receptors involved in inositol trisphosphate and cyclic GMP accumulation during the primary culture of adrenal chromaffin cells.

Authors:  T Nakaki; N Sasakawa; S Yamamoto; R Kato
Journal:  Biochem J       Date:  1988-04-15       Impact factor: 3.857

Review 2.  Metabolism of the inositol phosphates produced upon receptor activation.

Authors:  S B Shears
Journal:  Biochem J       Date:  1989-06-01       Impact factor: 3.857

3.  Inositol phosphate release and steroidogenesis in rat adrenal glomerulosa cells. Comparison of the effects of endothelin, angiotensin II and vasopressin.

Authors:  E A Woodcock; P J Little; J K Tanner
Journal:  Biochem J       Date:  1990-11-01       Impact factor: 3.857

4.  Calcium modulates the generation of inositol 1,3,4-trisphosphate in human platelets by the activation of inositol 1,4,5-trisphosphate 3-kinase.

Authors:  J L Daniel; C A Dangelmaier; J B Smith
Journal:  Biochem J       Date:  1988-08-01       Impact factor: 3.857

5.  A model of cytosolic calcium regulation and autacoids production in vascular endothelial cell.

Authors:  A Y Wong; G A Klassen
Journal:  Basic Res Cardiol       Date:  1992 Jul-Aug       Impact factor: 17.165

6.  Reduction in Gh protein expression is associated with cytodifferentiation of vascular smooth muscle cells.

Authors:  E Vincan; C B Neylon; A N Jacobsen; E A Woodcock
Journal:  Mol Cell Biochem       Date:  1996 Apr 12-26       Impact factor: 3.396
  6 in total

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