Karla-Mayra Rezende1, Marcelo Bönecker1, Luciana Côrrea2, Carlos-Alberto Perez3, Giancarlo-Espósito-de Souza Brito4, Gabriela-Oliveira Berti1, Andrea-Mantesso Pobocik5. 1. Universidade de São Paulo - USP, School of Dentistry, Department of Pediatric Dentistry and Orthodontics, São Paulo, SP, Brazil. 2. Pathology Department, School of Dentistry, University of São Paulo, Brazil. 3. XRF Line, Brazilian Synchrotron Light Laboratory, Campinas, Brazil. 4. University of São Paulo, Institute of Physics, Department of Applied Physics, Rua do Matão Travessa R #187, 05508-090 São Paulo, SP, Brazil. 5. Department of Pathology, Dentistry School of University of Sao Paulo, Sao Paulo, Brazil.
Abstract
BACKGROUND: Dental Mesenchymal stem cells has prompted great for cell-based therapeutics. But no one knows for sure what the true potential of these cells, since most of the studies were done in isolation, using as source, different donors or different cell processing conditions. MATERIAL AND METHODS: An enriched population of cells positive for CD146, STRO-1, and CD90 was isolated of third molars teeth indicated for extraction of patient with of 16 years old. Analysis of cell kinetics, and subcellular tests were performed to assess the presence of minor and trace elements by using synchrotron radiation x-ray fluorescence microscopy. RESULTS: In the cell kinetics assays, the enriched populations showed generally slower growth as compared to those that were non-enriched. In comparison between the pulp and papilla populations, the derived pulp grew more rapidly than that derived from the papilla. The CD90 + cells exhibited a smaller pulp area compared to other populations, but the papilla of these cells exhibited a larger area. The CD90 + cells exhibited higher amounts of P, S, Cl, K, and Ca, while the Cu and Zn exhibited more than CD146-. STRO1 - exhibited K and Cu. For both the pulp and the papilla, multipotent stem cells positive for all three markers were present. CONCLUSIONS: Although they have been obtained from the same tooth and donor, as well as were grown, the populations derived from these two tissues have different growth morphology and kinetics. The biochemical differences show different metabolic patterns, reflecting in part the growth differences. Key words:Synchrotron radiation, dental stem cells, mesenchymal stem cells, chemical composition. Copyright:
BACKGROUND: Dental Mesenchymal stem cells has prompted great for cell-based therapeutics. But no one knows for sure what the true potential of these cells, since most of the studies were done in isolation, using as source, different donors or different cell processing conditions. MATERIAL AND METHODS: An enriched population of cells positive for CD146, STRO-1, and CD90 was isolated of third molars teeth indicated for extraction of patient with of 16 years old. Analysis of cell kinetics, and subcellular tests were performed to assess the presence of minor and trace elements by using synchrotron radiation x-ray fluorescence microscopy. RESULTS: In the cell kinetics assays, the enriched populations showed generally slower growth as compared to those that were non-enriched. In comparison between the pulp and papilla populations, the derived pulp grew more rapidly than that derived from the papilla. The CD90 + cells exhibited a smaller pulp area compared to other populations, but the papilla of these cells exhibited a larger area. The CD90 + cells exhibited higher amounts of P, S, Cl, K, and Ca, while the Cu and Zn exhibited more than CD146-. STRO1 - exhibited K and Cu. For both the pulp and the papilla, multipotent stem cells positive for all three markers were present. CONCLUSIONS: Although they have been obtained from the same tooth and donor, as well as were grown, the populations derived from these two tissues have different growth morphology and kinetics. The biochemical differences show different metabolic patterns, reflecting in part the growth differences. Key words:Synchrotron radiation, dental stem cells, mesenchymal stem cells, chemical composition. Copyright:
Authors: Guiting Lin; Gang Liu; Lia Banie; Guifang Wang; Hongxiu Ning; Tom F Lue; Ching-Shwun Lin Journal: Stem Cells Dev Date: 2011-02-24 Impact factor: 3.272