Muhammed Yunus Bektay1, Eray Metin Güler2, Mustafa Gökçe3, Mustafa Volkan Kızıltaş4. 1. Bezmialem Vakıf University Faculty of Pharmacy, Department of Clinical Pharmacy; Marmara University Institute of Health Sciences, Department of Clinical Pharmacy, İstanbul, Turkey. 2. University of Health Sciences, Hamidiye Faculty of Medicine, Department of Medical Biochemistry; Haydarpaşa Numune Health Application and Research Center, Department of Medical Biochemistry, İstanbul, Turkey. 3. Bezmialem Vakıf University Faculty of Pharmacy, Department of Parhmacology, İstanbul, Turkey. 4. İstanbul University Faculty of Pharmacy, Department of Analytical Chemistry, İstanbul, Turkey.
Abstract
OBJECTIVES: Hepatocellular carcinoma (HCC) is the seventh most common cancer and the third leading cause of tumor-related deaths worldwide. Mechanisms underlying tumor onset, progression, and metastasis in the case of HCC have not been adequately studied. In this study, we aimed to investigate the genotoxic, cytotoxic, apoptotic and oxidant effects of olive leaf extract (OLE) on HCC cells. MATERIALS AND METHODS: H4IIE Rattus norvegicus hepatoma cells and Rattus norvegicus healthy liver clone-9 cells were treated with the increasing concentrations of OLEs (250-2000 ppm) in ethanol, acetone, dichloromethane, and methanol. ATP cell viability, intracellular reactive oxygen species generation levels, double staining test with acridine orange/ethidium bromide, comet assay, levels of interleukin 1-beta (IL-1β), IL-6, and tumor necrosis factor alpha were measured. Significance was determined using ANOVA test. RESULTS: Apoptotic, genotoxic, cytotoxic, and oxidative effects of OLEs increased with the increasing concentrations as compared to controls in H4IIE cells (p<0.001). CONCLUSION: This is the first study to show a significant and selective cytotoxic activity of OLEs in the selected H4IIE cancer cell lines. OLEs could selectively increase the apoptotic damage and show anti-proliferative and pro-apoptotic properties against the H4IIE cells. They could be recommended as potential nutraceuticals in the prevention of cancer.
OBJECTIVES: Hepatocellular carcinoma (HCC) is the seventh most common cancer and the third leading cause of tumor-related deaths worldwide. Mechanisms underlying tumor onset, progression, and metastasis in the case of HCC have not been adequately studied. In this study, we aimed to investigate the genotoxic, cytotoxic, apoptotic and oxidant effects of olive leaf extract (OLE) on HCC cells. MATERIALS AND METHODS: H4IIE Rattus norvegicus hepatoma cells and Rattus norvegicus healthy liver clone-9 cells were treated with the increasing concentrations of OLEs (250-2000 ppm) in ethanol, acetone, dichloromethane, and methanol. ATP cell viability, intracellular reactive oxygen species generation levels, double staining test with acridine orange/ethidium bromide, comet assay, levels of interleukin 1-beta (IL-1β), IL-6, and tumor necrosis factor alpha were measured. Significance was determined using ANOVA test. RESULTS: Apoptotic, genotoxic, cytotoxic, and oxidative effects of OLEs increased with the increasing concentrations as compared to controls in H4IIE cells (p<0.001). CONCLUSION: This is the first study to show a significant and selective cytotoxic activity of OLEs in the selected H4IIE cancer cell lines. OLEs could selectively increase the apoptotic damage and show anti-proliferative and pro-apoptotic properties against the H4IIE cells. They could be recommended as potential nutraceuticals in the prevention of cancer.
Authors: Zeinab Korany Hassan; Maha Hussein Elamin; Sawsan Ali Omer; Maha Hassan Daghestani; Ebtesam Salah Al-Olayan; Mai Abdelrahman Elobeid; Promy Virk Journal: Asian Pac J Cancer Prev Date: 2014-01