Literature DB >> 34973001

Extraction of Metabolites from Cancer Cells.

Elena Kochetkova1,2, Tina Becirovic1, Erik Norberg3.   

Abstract

Cancer cells possess an elevated demand for nutrients and metabolites due to their uncontrolled proliferation and need to survive in unfavorable conditions. Autophagy is a conservative degradation pathway that counters lack of nutrients and provides organelle and protein quality control, beyond maintenance of cellular metabolism.Mass spectrometry-based metabolomics is a powerful tool to study the metabolome of a cell. Such analysis requires proper sample preparation including the extraction of metabolites. Here, we provide a protocol for the extraction of metabolites from adherent cancer cells suitable for global metabolome profiling by mass spectrometry.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Autophagy; CE-MS; Cancer metabolism; Chaperone-mediated autophagy; GC-MS; LC-MS; Mass spectrometry; Metabolism; Methanol extraction

Mesh：

Year:  2022        PMID： 34973001     DOI： 10.1007/978-1-0716-2071-7_20

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  PHGDH Defines a Metabolic Subtype in Lung Adenocarcinomas with Poor Prognosis.

Authors:  Boxi Zhang; Adi Zheng; Per Hydbring; Gorbatchev Ambroise; Amanda Tomie Ouchida; Michel Goiny; Helin Vakifahmetoglu-Norberg; Erik Norberg
Journal:  Cell Rep       Date:  2017-06-13       Impact factor: 9.423

2.  Comparative evaluation of extraction methods for simultaneous mass-spectrometric analysis of complex lipids and primary metabolites from human blood plasma.

Authors:  Do Yup Lee; Tobias Kind; Young-Ran Yoon; Oliver Fiehn; Kwang-Hyeon Liu
Journal:  Anal Bioanal Chem       Date:  2014-08-30       Impact factor: 4.142

3.  Effects of harvesting and extraction methods on metabolite recovery from adherently growing mammalian cells.

Authors:  Yun Luo; Ningbo Geng; Baoqin Zhang; Jiping Chen; Haijun Zhang
Journal:  Anal Methods       Date:  2020-05-21       Impact factor: 2.896

4.  Regulation of glycolytic metabolism by autophagy in liver cancer involves selective autophagic degradation of HK2 (hexokinase 2).

Authors:  Lin Jiao; Hai-Liang Zhang; Dan-Dan Li; Ke-Li Yang; Jun Tang; Xuan Li; Jiao Ji; Yan Yu; Rui-Yan Wu; Senthilkumar Ravichandran; Jian-Jun Liu; Gong-Kan Feng; Min-Shan Chen; Yi-Xin Zeng; Rong Deng; Xiao-Feng Zhu
Journal:  Autophagy       Date:  2017-12-17       Impact factor: 16.016

Review 5.  Autophagy in the pathogenesis of disease.

Authors:  Beth Levine; Guido Kroemer
Journal:  Cell       Date:  2008-01-11       Impact factor: 41.582

6.  Simultaneous extraction of proteins and metabolites from cells in culture.

Authors:  Sean C Sapcariu; Tamara Kanashova; Daniel Weindl; Jenny Ghelfi; Gunnar Dittmar; Karsten Hiller
Journal:  MethodsX       Date:  2014-07-18

7.  The deubiquitinase JOSD2 is a positive regulator of glucose metabolism.

Authors:  Lyudmila Krassikova; Boxi Zhang; Divya Nagarajan; André Lima Queiroz; Merve Kacal; Evangelos Samakidis; Helin Vakifahmetoglu-Norberg; Erik Norberg
Journal:  Cell Death Differ       Date:  2020-10-20       Impact factor: 15.828

8.  Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death.

Authors:  Hong-Guang Xia; Ayaz Najafov; Jiefei Geng; Lorena Galan-Acosta; Xuemei Han; Yuan Guo; Bing Shan; Yaoyang Zhang; Erik Norberg; Tao Zhang; Lifeng Pan; Junli Liu; Jonathan L Coloff; Dimitry Ofengeim; Hong Zhu; Kejia Wu; Yu Cai; John R Yates; Zhengjiang Zhu; Junying Yuan; Helin Vakifahmetoglu-Norberg
Journal:  J Cell Biol       Date:  2015-08-31       Impact factor: 10.539

9.  Autophagy provides metabolic substrates to maintain energy charge and nucleotide pools in Ras-driven lung cancer cells.

Authors:  Jessie Yanxiang Guo; Xin Teng; Saurabh V Laddha; Sirui Ma; Stephen C Van Nostrand; Yang Yang; Sinan Khor; Chang S Chan; Joshua D Rabinowitz; Eileen White
Journal:  Genes Dev       Date:  2016-08-11       Impact factor: 11.361

10.  Glycolytic suppression dramatically changes the intracellular metabolic profile of multiple cancer cell lines in a mitochondrial metabolism-dependent manner.

Authors:  Reika Shiratori; Kenta Furuichi; Masashi Yamaguchi; Natsumi Miyazaki; Haruna Aoki; Hiroji Chibana; Kousei Ito; Shigeki Aoki
Journal:  Sci Rep       Date:  2019-12-10       Impact factor: 4.379
  10 in total
  1 in total

Review 1.  Metabolomics Research in Periodontal Disease by Mass Spectrometry.

Authors:  Sachio Tsuchida; Tomohiro Nakayama
Journal:  Molecules       Date:  2022-04-30       Impact factor: 4.927
  1 in total

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