Literature DB >> 34972948

Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin.

Sally P Wheatley1.   

Abstract

This paper describes an easy method to enrich the harvest of adherent mammalian cells at each stage of mitosis (from prometaphase to cytokinesis) by combining Eg5 inhibition using dimethylenastron (DMA) with mitotic shake-off, followed by timed release from the drug.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Dimethylenastron (DMA); Eg5; Mammalian; Mitotic synchrony; Survivin

Mesh：

Substances：

Year:  2022        PMID： 34972948     DOI： 10.1007/978-1-0716-1904-9_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  c-Src but not Fyn promotes proper spindle orientation in early prometaphase.

Authors:  Yuji Nakayama; Yuki Matsui; Yumi Takeda; Mai Okamoto; Kohei Abe; Yasunori Fukumoto; Naoto Yamaguchi
Journal:  J Biol Chem       Date:  2012-06-11       Impact factor: 5.157

2.  Inhibitors of kinesin Eg5: antiproliferative activity of monastrol analogues against human glioblastoma cells.

Authors:  Christine Müller; Dietmar Gross; Vasiliki Sarli; Michael Gartner; Athanassios Giannis; Günther Bernhardt; Armin Buschauer
Journal:  Cancer Chemother Pharmacol       Date:  2006-05-16       Impact factor: 3.333

3.  Validating the mitotic kinesin Eg5 as a therapeutic target in pancreatic cancer cells and tumor xenografts using a specific inhibitor.

Authors:  Min Liu; Haiyang Yu; Lihong Huo; Jianchao Liu; Minggang Li; Jun Zhou
Journal:  Biochem Pharmacol       Date:  2008-05-04       Impact factor: 5.858

4.  Cell Synchronization by Double Thymidine Block.

Authors:  Guo Chen; Xingming Deng
Journal:  Bio Protoc       Date:  2018-09-05

Review 5.  The Aurora B Kinase in Chromosome Bi-Orientation and Spindle Checkpoint Signaling.

Authors:  Veronica Krenn; Andrea Musacchio
Journal:  Front Oncol       Date:  2015-10-16       Impact factor: 6.244

Review 6.  Thirty years of search and capture: The complex simplicity of mitotic spindle assembly.

Authors:  Rebecca Heald; Alexey Khodjakov
Journal:  J Cell Biol       Date:  2015-12-14       Impact factor: 10.539
  6 in total

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