Literature DB >> 30263905

Cell Synchronization by Double Thymidine Block.

Guo Chen1, Xingming Deng1.   

Abstract

Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at G1/S boundary by using double thymidine block. After release into normal medium, cell population at distinct cell cycle phase could be collected at different time points.

Entities:  

Keywords:  Cell cycle; Cell synchronization; DNA replication; DNA synthesis; Thymidine

Year:  2018        PMID： 30263905      PMCID： PMC6156087          DOI： 10.21769/BioProtoc.2994

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


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