Athina G Mantelou1, Argyro Zacharioudaki2, George Pappas-Gogos1, Apostolos Papalois2,3, Alexandra Papoudou-Bai4, Anna Goussia4, Georgios K Glantzounis5. 1. Department of Surgery, University Hospital of Ioannina and School of Medicine, University of Ioannina, Ioannina, Greece. 2. Experimental, Educational and Research Center ELPEN, Athens, Greece. 3. European University Cyprus, School of Medicine, Nicosia, Cyprus. 4. Department of Pathology, Ioannina University Hospital and School of Medicine, University of Ioannina, Ioannina, Greece. 5. Department of Surgery, University Hospital of Ioannina and School of Medicine, University of Ioannina, Ioannina, Greece; gglantzounis@uoi.gr.
Abstract
AIM: Description of an anesthetic recovery model with endotracheal intubation in rabbits which provides metabolic stability for the study of the late phase of liver ischemia/reperfusion (I/R) injury. MATERIALS AND METHODS: Two groups of New Zealand rabbits, n=7 in each, were used: Ischemia/reperfusion (I/R) group (45 min of partial liver ischemia/reperfusion) and no intervention (sham) group. Blood alanine aminotransferase, lactate, pH values, mean arterial pressure and pCO2 were calculated at baseline, and at 2 and 24 h post reperfusion. Tissue samples from left (ischemic) and right (non-ischemic) liver lobes were examined at 2 and 24 h after reperfusion. RESULTS: The I/R group presented significantly higher levels of alanine aminotransferase (p=0.001) at 2 and 24 h, and of lactate (p=0.016) at 2 h post reperfusion. No differences were documented for pH, mean arterial pressure and pCO2 Histological exanimation revealed significant injury at 24 h post reperfusion for the I/R group. CONCLUSION: This anesthetic recovery model permitted avoidance of hypoxia and respiratory acidosis, allowing the study of the late phase of I/R injury.
AIM: Description of an anesthetic recovery model with endotracheal intubation in rabbits which provides metabolic stability for the study of the late phase of liver ischemia/reperfusion (I/R) injury. MATERIALS AND METHODS: Two groups of New Zealand rabbits, n=7 in each, were used: Ischemia/reperfusion (I/R) group (45 min of partial liver ischemia/reperfusion) and no intervention (sham) group. Blood alanine aminotransferase, lactate, pH values, mean arterial pressure and pCO2 were calculated at baseline, and at 2 and 24 h post reperfusion. Tissue samples from left (ischemic) and right (non-ischemic) liver lobes were examined at 2 and 24 h after reperfusion. RESULTS: The I/R group presented significantly higher levels of alanine aminotransferase (p=0.001) at 2 and 24 h, and of lactate (p=0.016) at 2 h post reperfusion. No differences were documented for pH, mean arterial pressure and pCO2 Histological exanimation revealed significant injury at 24 h post reperfusion for the I/R group. CONCLUSION: This anesthetic recovery model permitted avoidance of hypoxia and respiratory acidosis, allowing the study of the late phase of I/R injury.
Authors: Georgios K Glantzounis; Sophie A Rocks; Hemant Sheth; Iona Knight; Henryk J Salacinski; Brian R Davidson; Paul G Winyard; Alexander M Seifalian Journal: Free Radic Biol Med Date: 2006-12-22 Impact factor: 7.376
Authors: Niteen Tapuria; Sameer Junnarkar; Mahmoud Abu-Amara; Barry Fuller; Alexander M Seifalian; Brian R Davidson Journal: HPB (Oxford) Date: 2011-11-27 Impact factor: 3.647